Title:
CONSTRUCTION METHOD AND USE OF ASTAXANTHIN SYNTHESIS PATHWAY IN CHLAMYDOMONAS REINHARDTII
Document Type and Number:
WIPO Patent Application WO/2024/026963
Kind Code:
A1
Abstract:
Provided are a construction method and use of an astaxanthin synthesis pathway in Chlamydomonas reinhardtii. The construction method comprises design and assembly of an astaxanthin synthesis module and functional verification of the astaxanthin synthesis module in Chlamydomonas reinhardtii. Seven genes related to astaxanthin synthesis are selected, and an astaxanthin expression module framework is designed and divided into four fragments for artificial synthesis; then the four fragments and a yeast-E. coli shuttle plasmid backbone are co-transfected into a yeast to obtain a yeast containing the astaxanthin synthesis module; then the yeast containing the astaxanthin synthesis module is hybridized with a yeast containing a chloroplast genome to insert the astaxanthin synthesis module into a Chlamydomonas reinhardtii chloroplast genome, a recombinant plasmid is introduced into the Chlamydomonas reinhardtii chloroplast, and finally a positive Chlamydomonas reinhardtii transformant containing the astaxanthin synthesis module is obtained, namely an astaxanthin synthesis engineering algae strain. The finally obtained transgenic astaxanthin synthetic engineering algae strain can realize rapid and cheap production of astaxanthin by means of large-scale culturing.
Inventors:
HU ZHANGLI (CN)
MEI RUI (CN)
WANG CHAOGANG (CN)
ZHANG GUIYING (CN)
GUO CHUNLI (CN)
MEI RUI (CN)
WANG CHAOGANG (CN)
ZHANG GUIYING (CN)
GUO CHUNLI (CN)
Application Number:
PCT/CN2022/116578
Publication Date:
February 08, 2024
Filing Date:
September 01, 2022
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C12N15/82; C12N15/53; C12N15/54; C12N15/61; C12P23/00
Domestic Patent References:
| WO2005010156A2 | 2005-02-03 |
Foreign References:
| CN115807026A | 2023-03-17 | |||
| CN105316357A | 2016-02-10 | |||
| CN107129995A | 2017-09-05 | |||
| US20040268439A1 | 2004-12-30 |
Other References:
TAN CONGPING, QIN SONG: "Metabolic Engineering of Caroteniods", FOOD AND FERMENTATION INDUSTRIES, vol. 34, no. 3, 31 March 2008 (2008-03-31), pages 120 - 125, XP093135037, DOI: 10.13995/j.cnki.11-1802/ts.2008.03.038
WANG, GUODONG: "Utilization of Chlamydomonas as a Bioreactor for Astaxanthin Production", MASTER’S THESES OF QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY, 2020-10-26
PARK SEON YOUNG; BINKLEY ROBERT M.; KIM WON JUN; LEE MUN HEE; LEE SANG YUP: "Metabolic engineering ofEscherichia colifor high-level astaxanthin production with high productivity", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 49, 7 August 2018 (2018-08-07), AMSTERDAM, NL, pages 105 - 115, XP085507119, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2018.08.002
WANG, GUODONG: "Utilization of Chlamydomonas as a Bioreactor for Astaxanthin Production", MASTER’S THESES OF QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY, 2020-10-26
PARK SEON YOUNG; BINKLEY ROBERT M.; KIM WON JUN; LEE MUN HEE; LEE SANG YUP: "Metabolic engineering ofEscherichia colifor high-level astaxanthin production with high productivity", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 49, 7 August 2018 (2018-08-07), AMSTERDAM, NL, pages 105 - 115, XP085507119, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2018.08.002
Attorney, Agent or Firm:
JOHNSON INTELLECTUAL PROPERTY AGENCY(SHENZHEN) (CN)
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