Title:
CONSTRUCTION METHOD AND USE OF GASTRULOID STEM CELL LINE
Document Type and Number:
WIPO Patent Application WO/2024/060465
Kind Code:
A1
Abstract:
The present invention provides a construction method and use of a gastruloid stem cell line. A gastruloid stem cell that can be stably passaged is established in this research, which retains the potential of stem cells to a certain extent and shows the expression of genes and proteins of entoderm, mesoderm and ectoderm cells in a gastrula development stage. The characteristics of the gastruloid stem cell are consistent with those of cells in a gastrula stage, so that the key characteristics of the cells in the gastrula stage can be well reproduced. By using the stem cell line, a three-dimensional gastruloid model capable of simulating the development of gastrula can be constructed in mice or in vitro, and key biological events such as the separation of endodermal and ectodermal lineages, the formation of a pro-amniotic cavity, the occurrence of a primitive streak, and the specialization of a mesodermal lineage in an in-vivo embryonic development process can be partially reproduced, so that key characteristics of an embryo in a gastrula stage after implantation can be well reproduced. By using the model, a drug screening platform influencing early embryonic development can be established in vitro, which provides a reference for clinical medication.
Inventors:
SHA JIAHAO (CN)
YUAN YAN (CN)
HUANG MINGQIAN (CN)
CHEN MENGQI (CN)
ZHANG HAO (CN)
YUAN GEGE (CN)
YUAN YAN (CN)
HUANG MINGQIAN (CN)
CHEN MENGQI (CN)
ZHANG HAO (CN)
YUAN GEGE (CN)
Application Number:
PCT/CN2022/143644
Publication Date:
March 28, 2024
Filing Date:
December 29, 2022
Export Citation:
Assignee:
UNIV NANJING MEDICAL (CN)
International Classes:
C12N5/0735; C12N5/074
Domestic Patent References:
| WO2017175866A1 | 2017-10-12 |
Foreign References:
| US20180187147A1 | 2018-07-05 | |||
| US20100173414A1 | 2010-07-08 | |||
| US20160038546A1 | 2016-02-11 | |||
| US20200032215A1 | 2020-01-30 |
Other References:
ZHOU QUAN; WANG MEI; YUAN YAN; WANG XUEPENG; FU RUI; WAN HAIFENG; XIE MINGMING; LIU MINGXI; GUO XUEJIANG; ZHENG YING; FENG GUIHAI;: "Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro", CELL STEM CELL, ELSEVIER, CELL PRESS, AMSTERDAM, NL, vol. 18, no. 3, 1 January 1900 (1900-01-01), AMSTERDAM, NL , pages 330 - 340, XP029451696, ISSN: 1934-5909, DOI: 10.1016/j.stem.2016.01.017
Attorney, Agent or Firm:
NANJING TIANHUA PATENT AGENT CO., LTD. (CN)
Download PDF: