Title:
CULTURE METHOD AND CULTURE VESSEL
Document Type and Number:
WIPO Patent Application WO/2021/015213
Kind Code:
A1
Abstract:
In a culture vessel having a plurality of wells, culturing can be performed under optimum culture conditions according to the types of cells to be cultured in each well. This culture method comprises: filling, with a nerve cell medium, a first well 1 of a culture vessel, and a second well 2 communicating with the first well 1 through a flow path 3; seeding a nerve cell N in the first well 1; culturing the axon a of the nerve cell N until the axon a of the nerve cell N reaches the second well 2 through the flow path 3 and blocks the flow path 3; then removing the medium in the second well 2 and filling the second well 2 with a medium for a co-cultured cell that is co-cultured with the nerve cell N; and seeding a cell C to be co-cultured in the second well 2, and culturing the cell C together with the axon a of the nerve cell N.
Inventors:
KAWADA JIRO (JP)
KASAI AKIRA (JP)
KASAI AKIRA (JP)
Application Number:
PCT/JP2020/028338
Publication Date:
January 28, 2021
Filing Date:
July 21, 2020
Export Citation:
Assignee:
JIKSAK BIOENGINEERING INC (JP)
ZEON CORP (JP)
ZEON CORP (JP)
International Classes:
C12M1/00; C12M1/18; C12M3/00; C12N5/079; C12N5/0793
Domestic Patent References:
| WO2017187696A1 | 2017-11-02 | |||
| WO2017187696A1 | 2017-11-02 |
Foreign References:
| JP2019000093A | 2019-01-10 | |||
| JP2013135637A | 2013-07-11 | |||
| JP2013021946A | 2013-02-04 | |||
| US20100323434A1 | 2010-12-23 | |||
| US20170355945A1 | 2017-12-14 | |||
| JP2012504949A | 2012-03-01 | |||
| JP2018536424A | 2018-12-13 |
Other References:
YUMOTO NORIHIRO ET AL.: "The nerve organoid culture devices", SAIBOU - THE CELL, vol. 51, no. 4, 20 April 2019 (2019-04-20), JP , pages 29 - 31, XP009533462, ISSN: 1346-7557
KAMADA GIRO ET AL: "New approaches for investigation and pharmaceutical development of neurodegenerative diseases", SEISAN KENKYU, vol. 68, no. 3, 2016, pages 205 - 210, XP055786070
AKIYAMA, T. ET AL.: "Aberrant axon branching via Fos-B dysregulation in FUS- ALS motor neurons", EBIOMEDICINE, vol. 45, 29 June 2019 (2019-06-29), pages 362 - 378, XP055743312, DOI: 10.1016/j.ebiom.2019.06.013
YUMOTO ET AL.: "Lrp4 is a retrograde signal for presynaptic differentiation at neuromuscular synapses", NATURE, vol. 489, 2012, pages 438 - 442
KAMADA GIRO ET AL: "New approaches for investigation and pharmaceutical development of neurodegenerative diseases", SEISAN KENKYU, vol. 68, no. 3, 2016, pages 205 - 210, XP055786070
AKIYAMA, T. ET AL.: "Aberrant axon branching via Fos-B dysregulation in FUS- ALS motor neurons", EBIOMEDICINE, vol. 45, 29 June 2019 (2019-06-29), pages 362 - 378, XP055743312, DOI: 10.1016/j.ebiom.2019.06.013
YUMOTO ET AL.: "Lrp4 is a retrograde signal for presynaptic differentiation at neuromuscular synapses", NATURE, vol. 489, 2012, pages 438 - 442
Attorney, Agent or Firm:
SUGIMURA Kenji (JP)
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