Title:
DNA CONSTRUCTS BASED ON THE eIF4A GENE PROMOTER
Document Type and Number:
WIPO Patent Application WO2001002594
Kind Code:
A3
Abstract:
The present invention provides novel DNA constructs comprising a transcriptional regulatory sequence comprising a polynucleotide derivable from the eIF4A1 gene promoter. In preferred embodiments, the polynucleotide further comprises a polynucleotide derivable from the eIF4A gene introns, particularly intron 1. Host cells harbouring the constructs are also provided. These novel constructs have applications in gene therapy, DNA vaccines and in the commercial production of proteins.
More Like This:
Inventors:
GREAVES DAVID ROBERT (GB)
THOMSEN LINDY (GB)
CATCHPOLE IAN RICHARD (GB)
FORD MARTIN JAMES (GB)
THOMSEN LINDY (GB)
CATCHPOLE IAN RICHARD (GB)
FORD MARTIN JAMES (GB)
Application Number:
PCT/GB2000/002569
Publication Date:
November 15, 2001
Filing Date:
July 05, 2000
Export Citation:
Assignee:
GLAXO GROUP LTD (GB)
GREAVES DAVID ROBERT (GB)
THOMSEN LINDY (GB)
CATCHPOLE IAN RICHARD (GB)
FORD MARTIN JAMES (GB)
GREAVES DAVID ROBERT (GB)
THOMSEN LINDY (GB)
CATCHPOLE IAN RICHARD (GB)
FORD MARTIN JAMES (GB)
International Classes:
C12N15/09; A61K35/76; A61K38/00; A61K39/395; A61K48/00; A61P35/00; A61P43/00; C07K14/47; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; C12N15/85; C12P21/00; A61K39/00; (IPC1-7): C12N15/85; A61K31/17; C12N5/10; C12N15/12
Domestic Patent References:
| WO1997042337A1 | 1997-11-13 |
Other References:
KUKIMOTO I. ET AL.: "Characterization of the cloned promoter of the human initiation factor 4AI gene", BIOCHEM. BIOPHYS. RES. COM., vol. 233, 1997, pages 844 - 847, XP002152479
QUINN C.M. ET AL.: "The human eukaryotic initiation factor 4AI gene (EIF4AI) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines", GENOMICS, vol. 62, 15 December 1999 (1999-12-15), pages 468 - 476, XP002152480
DATABASE EMBL SEQ. 6 August 1995 (1995-08-06), "EST; H. sapiens cDNA clone IMAGE:187152", XP002152483
DATABASE EMBL SEQUENCES 7 October 1995 (1995-10-07), "EST; H. sapiens cDNA clone IMAGE:204614 similar ro eukaryotic initiation factor 4A-I.", XP002152484
DATABASE EMBL SEQUENCES 14 December 1996 (1996-12-14), ANDERSSON B.: "Human clone 23933 mRNA", XP002152485
DATABASE EMBL SEQUENCES 10 March 1998 (1998-03-10), "Mouse eIF4A gene", XP002152486
JONES E. ET AL.: "The linked human elongation initiation factor 4A1 (eIF4A1) and CD68 genes map to chromosome 17p13", GENOMICS, vol. 53, 15 October 1998 (1998-10-15), pages 248 - 250, XP002157483
QUINN C.M. ET AL.: "The human eukaryotic initiation factor 4AI gene (EIF4AI) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines", GENOMICS, vol. 62, 15 December 1999 (1999-12-15), pages 468 - 476, XP002152480
DATABASE EMBL SEQ. 6 August 1995 (1995-08-06), "EST; H. sapiens cDNA clone IMAGE:187152", XP002152483
DATABASE EMBL SEQUENCES 7 October 1995 (1995-10-07), "EST; H. sapiens cDNA clone IMAGE:204614 similar ro eukaryotic initiation factor 4A-I.", XP002152484
DATABASE EMBL SEQUENCES 14 December 1996 (1996-12-14), ANDERSSON B.: "Human clone 23933 mRNA", XP002152485
DATABASE EMBL SEQUENCES 10 March 1998 (1998-03-10), "Mouse eIF4A gene", XP002152486
JONES E. ET AL.: "The linked human elongation initiation factor 4A1 (eIF4A1) and CD68 genes map to chromosome 17p13", GENOMICS, vol. 53, 15 October 1998 (1998-10-15), pages 248 - 250, XP002157483
Download PDF:
Previous Patent: CONTROL OF GENE EXPRESSION IN EUKARYOTIC CELLS
Next Patent: METHOD FOR OXIDATING ORGANIC COMPOUNDS
Next Patent: METHOD FOR OXIDATING ORGANIC COMPOUNDS