Title:
AN E. coli EXPRESSED RECOMBINANT HUMAN MICROPLASMIN, A PRODUCTION METHOD THEREOF, A PHARMACEUTICAL COMPOSITION AND A KIT COMPRISING THEREOF, A METHOD AND AN APPLICATION OF TREATING THROMBOEMBOLISM RELATED DISEASES BY USING THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/185918
Kind Code:
A1
Abstract:
Provided is an E. coli expressed recombinant human microplasmin (E-μPlm), a production method thereof, a pharmaceutical composition and a kit comprising thereof, an application of the pharmaceutical composition and the kit in treating thromboembolism related diseases and a method of treating thromboembolism related diseases by using the pharmaceutical composition and the kit. The zymogen version of the E-μPlm or E-μPlg is unstable and can undergo autoactivation. Moreover, the in vivo half-life of the E-μPlm is significantly shorter than yeast expressed microplasmin (Y-μPlm), which allows the E-μPlm, the pharmaceutical composition and the kit effective in treating thromboembolism diseases but with reduced bleeding side effects.
Inventors:
LIN XINLI HENRY (US)
Application Number:
PCT/CN2023/084665
Publication Date:
October 05, 2023
Filing Date:
March 29, 2023
Export Citation:
Assignee:
SHENZHEN BAY LABORATORY (CN)
International Classes:
C12N9/68; A61K38/48; A61P7/02; C07K14/47; C12N15/10; C12N15/57; C12N15/70; C12R1/19
Foreign References:
| US20210330762A1 | 2021-10-28 | |||
| CN102660564A | 2012-09-12 | |||
| CN102199587A | 2011-09-28 | |||
| CN102482338A | 2012-05-30 | |||
| CN103205410A | 2013-07-17 |
Other References:
YANG DONGYING, ZHU WEI, WANG YINGJIE, TAN FANGMEI, MA ZHIPING, GAO JIALI, LIN XINLI: "Selection of mutant µplasmin for amyloid-β cleavage in vivo", SCIENTIFIC REPORTS, vol. 10, no. 1, XP093096419, DOI: 10.1038/s41598-020-69079-8
XIAOTAO LIN ET AL: "Purification and characterization of mutant miniPlasmin for thrombolytic therapy", THROMB J., vol. 11, no. 1, 30 January 2013 (2013-01-30), XP021142029, DOI: 10.1186/1477-9560-11-2
XIAOTAO LIN ET AL: "Purification and characterization of mutant miniPlasmin for thrombolytic therapy", THROMB J., vol. 11, no. 1, 30 January 2013 (2013-01-30), XP021142029, DOI: 10.1186/1477-9560-11-2
Attorney, Agent or Firm:
SHENZHEN ELSON PATENT AGENT FIRM (GENERAL PARTNERSHIP) (CN)
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