BACKGROUND OF THE INVENTION The information provided and the references cited herein are not admitted, nor should they be construed, to be prior art to the present invention; rather, they are provided solely to assist the reader in understanding the present invention.

Efflux pumps are protein-driven mechanisms by means of which cells expel xenobiotics, including unfortunately, those which might benefit the organism hosting the cells. Since their postulation in the 1970s as a mechanism of bacterial resistance to drugs (Levy, S. B. and McMurray, L., Nature, 1978,276 : 90), efflux pumps have been found to be ubiquitous in all types of cells, from bacteria to fungi to mammalian. (Higgins, Ann. Rev. Cell Biol., 1992,8 : 67-113). Due to their pervasiveness, efflux pumps have risen to prominence as a leading cause of resistance to pharmaceuticals in a host of pathological scenarios.

While fungal infections are relatively rare in immuno-competent patients, they can be life threatening for immuno-compromised patients of which there are three major groups: (1) cancer patients undergoing chemotherapy, (2) organ transplant patients being treated with immuno-suppressants, and (3) AIDS patients. Data from the National Nosocomial Infections Surveillance System conducted in the United States showed a 487 percent increase in Candida bloodstream infections between 1980 and 1989 (Rinaldi, et al., Antimicrob. Ag. Chemother., 1995,39 : 1-8).

Oropharyngeal candidiasis is the most common fungal infection complication associated with AIDS with up to 90% of AIDS patients having had at least one episode of the infection (Powderly, AIDS research and Human Retroviruses, 1994, 10: 925-929).

There are relatively few clinically useful anti-fungal agents. Among those available are amphotericin B, flucytosine, fluconazole, itraconazole and ketoconazole (Odds, J. Antimicrob. Chemother., 1993,31 : 463-471). However, resistance to all of these drugs is developing rapidly. For example, fluconazole is currently the most extensively used anti-fungal agent for the treatment of patients with severe candidiasis. Between 1988 and 1993, fluconazole was used to treat over 15 million patients, including at least 250,000 AIDS patients (Hitchcock, Biochem. Soc. Trans., 1993,21 : 1039-1047). Given such wide-spread use, it comes as no surprise that fluconazole-resistant Candida strains have been reported (Rex, et al., Antimicrob.

Ag. Chemother., 1995,39 : 1-8; Vanden Bossche, et al., 1994, supra). Fluconazole resistance in mutant strains of Candida appears to be primarily due to efflux of the drug (Vanden Bossche, et al., 1994; Odds, 1993, supra). Furthermore, species intrinsically resistant to fluconazole, such as C. glabrata, C. krusei and Aspergillus fumigatus, have also been shown to accumulate less fluconazole than susceptible species (van den Bossche, et al., 1994, supra) suggesting that both intrinsic and acquired resistance may be due to an efflux pump mechanism. In fact, multiple- drug resistant (MDR) efflux pumps have been implicated in fluconazole resistance in C. albicans and C. glabrata (Parkinson, et al., Antimicrob. Agents Chemother., 1995,39 : 1696-1699.

Based on the above, it would clearly be desirable to be able to inhibit the activity of fungal efflux pumps so that anti-fungal agents can accumulate in fungal cells in sufficient quantity to exert their effect. The present invention provides compounds that achieve this goal.

Efflux pumps in mammalian cells include P-glycoprotein (P-gp) and multidrug resistant associated protein (MRP-1) (Gottesman, et al., Ann. Rev. Biochem., 1993,62 : 385-427), both of which confer multiple drug resistance (MDR) on cells using them. P-gp is known to be highly expressed in approximately 50% of human cancer cells and is thus suspected to be key to the resistance of many cancers to chemotherapeutics. In fact, P-gp and MRP have been implicated in the failure of chemotheapeutic regimes in patients with a variety of cancers including haematological malignancies. While both pumps are ATP energy dependent, whereas P-gp transports lipophilic compounds, MRP-1 transports amphiphatic anions, including glutathione and glucuronide conjugates. Thus, a broad spectrum of chemotherapeutic drugs are substrates for P-gp and/or MRP-1. For example, anthracyclines such as doxorubicin and daunorubicin, vinca alkaloid such as vincistine, epipodophyllotoxins such as etoposide and the taxanes (paxlitaxel) are all effluxed by P-gp or MRP-1. In addition, a variety of other drugs have been shown to be effluxed by P-gp and MRP-1 including loperamide, morphin, digoxin, progesterone and ivermectin (P-gp substrates) and indomethacine, ceftriaxone, rifampicin, sulfinylpyrazone and 2, 4-dinitrophenyl-S-glutathione (MRP-1 substrates).

Furthermore, P-gp is present in the blood-brain barrier (BBB) and is known to adversely affect the therapeutic activity of several CNS drugs including phenytoin (anti-epileptic), clomipramine (anti-depressant), and chlorpromazine (neuroleptic).

P-gp and MRP-1 are known to be susceptible to inhibition by compounds such as the immunosuppresent Cyclosporin A, Valspodar, a non-immuno- suppressive cyclosporin and ritonavir, a HIV protease inhibitor. However, in many types odf cancers, in particular solid tumor cancers, such inhibitors have been disappointingly ineffective in overcoming resistance. Whether this is due simply to insufficient accumulation of the inhibitor in the cells or some other mechanism is not presently known. In any event, there is a need for compounds that are capable of inhibiting the P-gp and MRP-1 efflux pumps and potentiating chemotherapeutic drugs. The present invention provides compounds that are expected to inhibit these pumps as well as the fungal efflux pumps discussed above.

SUMMARY OF THE INVENTION The present invention relates to compounds that are efflux pump inhibitors. In one embodiment, when administered to a patient suffering from an infection caused by a fungal species that employs efflux pump (s) as a resistance mechanism, the compounds inhibit the activity of the pump (s) allowing a co-administrated anti-fungal agent to accumulate in sufficient concentration to inhibit fungal cells and treat the infection. In particular the compounds herein inhibit efflux pumps active in genus Candida fungi. In another embodiment, when administered to a patient suffering from a form a cancer, in particular a solid tumor cancer or leukemia, where the cancerous cells employ P-gp and/or MRP-1 efflux pump (s) as a resistance mechanism, the compounds inhibit the activity of the pump (s) allowing co- administered chemotherapeutic agent (s) to accumulate in sufficient concentration to inhibit the cancerous cells. In particular, the compounds of this invention are expected to inhibit the efflux pump proteins pGp and MRP-1, the compounds are expected to inhibit the activity of these pumps also and thereby allow accumulation of therapeutical effective amounts of chemotherapeutics in the cells resulting in cell death.

Thus, in one aspect the present invention relates to a compound having the chemical formula: or a pharmaceutical acceptable salt thereof, wherein: Ai is carbon or nitrogen, provided that when Ai is nitrogen, R5 does not exist; J is carbon or nitrogen; R2 and R3 are independently selected from the group consisting of hydrogen, halo, - (1 C-4C) alkyl, and-0 (1 C-4C) alkyl ; R4 and R6 are independently selected from the group consisting of hydrogen, halo, -O (1 C-4C) alkyl,-OCF3, and O-CH2 (3C-6C) cycloalkyl ; R5 is selected from the group consisting of hydrogen and R21 is selected from the group consisting of hydrogen and-NHSO2CH3 ; R7 and R3 are independently selected from the group consisting of hydrogen, halo, -C#N, -(1C-4C)alkyl, -O(1C-4C)alkyl, -OCHF2, -CF3, -OCF3 and, taken together, -OCH2O-; R'is selected from the group consisting of- (1 C-4C) alkyl,- (3C-6C) cycloalkyl, -CH2 (3C-6C) alkyl, -SO2R16, -SO2NR17R18, -C(O)OR17 and A4 is selected from the group consisting of-NH, oxygen and sulfur ; A2, As and A5 are independently selected from the group consisting of carbon and nitrogen provided that no more than two of A2, A3 and A5 are nitrogen at the same time; or, Ri is-C (O) (CHR22) nR9, wherein, n is 0, 1, 2 or 3 ; R9 is selected from the group consisting of hydrogen,-OH,- (1 C-4C) alkyl,- (3C-6C) cycloalkyl,-CH2 (3C-6C) cycloalkyl, -NR17R18, wherein: A6, A7, A8, An and A, 5 are independently selected from the group consisting of carbon, oxygen, sulfur and NR23 ; Ag, Alto, A12, A13 and A14 are independently selected from the group consisting of carbon and nitrogen; Rlo and R11 are independently selected from the group consisting of hydrogen,-(1 C-4C) alkyl,-SO2R16,-C (O) R16 and-C (O) OR wherein: R16 is selected from the group consisting of hydrogen and - (1 C-4C) alkyl wherein the alkyl group may be substituted with 1,2, 3, or 4 fluorines ; R12 is selected from the group consisting of hydrogen,- (1 C-4C) alkyl, - (3C-6C) cycloalkyl,-CH2 (3C-6C) cycloalkyl, -C(O)O-(1 C-4C) alkyl, - SO2R17 and -SO2NR18R19, wherein, R17, R18 and R19 are independently selected from the group consisting of hydrogen and -(1 C-4C) alkyl ; R14 and R15 are independently selected from the group consisting of hydrogen, -(1C-4C)alky and -NR10R11 ; R22 is independently selected from the group consisting of hydrogen and (1 C-4C) alkyl for each n carbon, that is, if n is 2 or 3, R22 may be independently hydrogen or (1 C-4C) alkyl for each of those carbons; R23 is selected from the group consisting of hydrogen, (1 C-4C) alkyl, - C (O) (1 C-4C) alkyl and-C (0) 0 (1 C-4C) alkyl or, R9 is-C (R16)(R20) (CH2) pNR10R11, wherein: p is 0, 1 or 2 ; R20 is selected from the group consisting of hydrogen and- (1 C- 4C) alkyl, the alkyl group being optionally substituted with an entity selected from the group consisting of-OH,-0 (1 C-4C) alkyl,-C-N,- S02 (1 C-4C) alkyl and the compound comprises a racemic mixture, a pure enantiomer or a pure atropisomer of either the racemic mixture or the pure enantiomer.

In an aspect of this invention, the compound is in the S absolute configuration at the starred carbon.

In an aspect of this invention, Ai is carbon.

In an aspect of this invention, J is nitrogen.

In an aspect of this invention, R7 is selected from the group consisting of hydrogen and halogen and R8 is hydrogen.

In an aspect of this invention, R7 is fluorine.

In an aspect of this invention, Ai and J are nitrogen.

In an aspect of this invention, R4 and R6 are independently selected from the group consisting of-0 (1 C-4Calkyl) and-OCH2 (3C-6C) cycloalkyl.

In an aspect of this invention, R4 and R6 are OCH3.

In an aspect of this invention, R7 is selected from the group consisting of hydrogen and fluorine.

In an aspect of this invention, R6 is selected from the group consisting of- R7 is F.

In an aspect of this invention, R6 is selected from the group consisting of- In an aspect of this invention, R4 and R6 are In an aspect of this invention R21 is -NHSO2CH3.

In an aspect of this invention, R5 is In an aspect of this invention, R'is- (1C-4C)alkyl.

In an aspect of this invention, R'is-CH3.

In an aspect of this invention, R1 is -C(O)(CHR22)nR9 and n is 0.

In an aspect of this invention, R1 is -C(O)(CHR22)NR9, wherein R9 is -C(R16)(R20) (CH2) pNR10R11, n is 0, p is 0 and R16 and R20 are independently selected from the group consisting of hydrogen and (1 C-4C) alkyl.

In an aspect of this invention, R16 and R20 are-CH3.

In an aspect of this invention, R1 is -C(O)(CHR22)nR9, R9 is -C (R) (R) (CH2) pNR R, n is 0, p is 0, R is (1 C-4C) alkyl, R and R20 are hydrogen and R11 is -C(O)O(1C-4C)alkyl.

In an aspect of this invention, R16 is methyl and R11 is-C (O) OCH3.

In an aspect of this invention, R1 is -C(O)(CHR22)nR9, n is 1, R22 is- (1C- 4C) alkyl and R9 is selected from the group consisting of-OH and In an aspect of this invention, R22 is-CH3.

In an aspect of this invention, R1 is A2 and A5 are nitrogen; A4 is sulfur ; and, A3 is carbon.

In an aspect of this invention, R7 is fluorine and R8 is hydrogen.

In an aspect of this invention, R2 and R3 are independently selected from the group consisting of hydrogen, chlorine and fluorine.

In an aspect of this invention, R2 is hydrogen and R3 is selected from the group consisting of hydrogen, chlorine and fluorine.

In an aspect of this invention, R2 and R3 are fluorine.

An aspect of this invention is a method for inhibiting a fungal cell that employs an efflux pump resistance mechanism, comprising contacting the cell with an anti- fungal agent whose activity depends on accumulation in the fungal cell and a compound hereof.

In an aspect of this invention, the anti-fungal agent is an azole anti-fungal agent.

In an aspect of this invention, the azole fungicide is selected from the group consisting of fluconazole, itraconazole, posaconazole, ravuconazole and voriconazole.

In an aspect of this invention, the fungal cell is first contacted with the compound and then with the anti-fungal agent.

In an aspect of this invention, the fungal cell is contacted with the compound and the anti-fungal agent simultaneously.

In an aspect of this invention, the fungal cell is a genus Candida cell.

In an aspect of this invention, the genus Candida cell is selected from the group consisting of C. albicans, C. krusei, C. tropicalis, C. parapsilosis and C. glabrata.

In an aspect of this invention, the fungal cell is a genus Aspergillus cell.

In an aspect of this invention, the genus Aspergillus cell is an Aspergillus fumigatus cell.

An aspect of this invention is a method for treating an infection caused by a fungus that employs an efflux pump resistance mechanism, comprising administering to a patient in need thereof a therapeutically effective amount of an anti-fungal agent whose activity depends on accumulation in cells of the fungus and a compound hereof.

In an aspect of this invention, the infection is caused by a genus Candida fungus.

In an aspect of this invention, the Candida fungus is C albicans, C. krusei, C. tropicalis, C. parapsilosis or C. glabrata.

In an aspect of this invention, the infection is caused by a genus Aspergillus fungus.

In an aspect of this invention, the genus Aspergillus fungus is Aspergillus fumigatus.

In an aspect of this invention, the compound and the anti-fungal agent are administered simultaneously.

In an aspect of this invention, the compound is administered first followed by administration of the anti-fungal agent.

An aspect of this invention is a pharmaceutical composition, comprising a pharmaceutically acceptable carrier or excipient and a compound hereof.

In an aspect of this invention, the pharmaceutical composition further comprises a therapeutically effective amount of an anti-fungal agent.

In an aspect of this invention, the anti-fungal agent is an azole anti-fungal agent.

In an aspect of this invention, the azole anti-fungal agent is fluconazole, itraconzole, posaconazole, ravuconazole and voriconazole.

An aspect of this invention is a method for treating a disease or disorder wherein cells involved in the disease or disorder employ an Pgp or MRP-1 efflux pump resistance mechanism, comprising administering to a patient in need thereof a therapeutically effective amount of an agent known to be effective against the disease or disorder in the absence of the efflux pump and a compound hereof.

In an aspect of this invention, the disease is leukemia.

In an aspect of this invention, the disease is cancer.

DETAILED DESCRIPTION OF THE INVENTION Brief description of the tables Table 1 shows representative racemic N-methylpiperazine compounds of this invention.

Table 2 shows representative racemic compounds of this invention other than N-methylpiperazines.

Table 3 shows representative S-absolute configuration compounds of this invention.

Table 4 provides data regarding the potentiation, by representative compounds of this invention, of fluconazole against a Candida albicans strain over- expressing CDR1 and CDR2 efflux pumps.

Table 5 provides data regarding the potentiation, by representative compounds of this invention, of fluconazole against a Candida glabrata strain over- expressing CgCDR1 and CgCDR2 efflux pumps.

Definitions As used herein, the term"alkyl"refers to a straight or branched chain saturated aliphatic hydrocarbon. Preferably, the alkyl group consists of 1 to 20 carbon atoms (whenever a numerical range such as"1-20"or"1 to 20"is provided herein, it means that the group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc. , up to and including 20 carbon atoms). More preferably, an alkyl group of this invention is a medium size alkyl having 1 to 10 carbon atoms. Most preferably, it is a lower alkyl having 1 to 4 carbon atoms. The size of an alkyl may be indicated by the formula (Ca-Cb) alkyl where a and b are integers from 1 to 20 and indicate how may carbons are in the alkyl chain. For example, a (Ci-C4) alkyl refers to a straight or branched chain alkyl consisting of 1,2, 3 or 4 carbon atoms. An alkyl group may be substituted or unsubstituted. When substituted, the substituent group (s) is preferably one or more independently selected from the group consisting of (C3-C6) cycloalkyl, halo, hydroxy, alkoxy, acyloxy, amino, acylamino, amido, carboxy, carbonyl, alkylcarbonyl, alkoxycarbonyl, cyano and nitro.

A"cycloalkyl"group refers to a 3 to 8 member all-carbon monocyclic ring.

The designation (C3-C6) cycloalkyl, for example, refers to a 3-, 4-, 5-or 6-member all- carbon ring. A cycloalkyl group may contain one or more double bonds but it does not contain a fully conjugated pi-electron system; i. e. , it is not aromatic. Examples, without limitation, of cycloalkyl groups are cyclopropane, cyclobutane, cyclopentane, cyclopentene, cyclohexane, cyclohexadiene, adamantane, cycloheptane and, cycloheptatriene. A cycloalkyl group may be substituted or unsubstituted. When substituted, the substituent group (s) is preferably one or more independently selected from the group consisting of unsubstituted (Ci-C4) alkyl, halo, hydroxy, alkoxy, acyloxy, amino, acylamino, amido, carboxy, carbonyl, alkylcarbonyl, alkoxycarbonyl, cyano and nitro.

An"alkenyl"group refers to an alkyl group, as defined herein, having at least two carbon atoms and at least one carbon-carbon double bond. As used herein, (C2- C4) alkenyl, for example, refers to a 2,3, or 4 carbon alkenyl group.

An"aryl"group refers to an all-carbon monocyclic or a fused-ring polycyclic (i. e. , rings which share adjacent pairs of carbon atoms) group having a completely conjugated pi-electron system. Examples, without limitation, of aryl groups are phenyl, naphthalenyl and anthracenyl. The aryl group may be substituted or unsubstituted. When substituted, the substituted group (s) is preferably one or more independently selected from the group consisting of alkyl, halo, (halo) 3C-, hydroxy, alkoxy, acyloxy, amino, acylamino, amido, carboxy, carbonyl, alkylcarbonyl, alkoxycarbonyl, cyano and nitro.

As used herein, a"heteroaryl"group refers to a monocyclic or fused ring in which one or more of the rings contains one or more atoms selected from the group consisting of nitrogen, oxygen and sulfur and, in addition, sufficient double bonds to establish a fully conjugated pi-electron system. Examples, without limitation, of heteroaryl groups are pyrrole, furan, thiophene, imidazole, oxazol, thiazole, pyrazol, pyridine, pyrimidine, quinoline, isoquinoline, purine and carbazole. A heteroaryl group may be substituted or unsubstituted. When substituted, the substituted group (s) is preferably one or more independently selected from the group consisting of alkyl, halo, (halo) 3C-, hydroxy, alkoxy, acyloxy, amino, acylamino, amido, carboxy, carbonyl, alkylcarbonyl, alkoxycarbonyl, cyano and nitro.

A"heteroalicyclic"group refers to a monocyclic or fused ring group having in the ring (s) one or more atoms selected from the group consisting of nitrogen, oxygen and sulfur. The rings may also have one or more double bonds. However, the rings do not have a completely conjugated pi-electron system. The heteroalicyclic ring may be substituted or unsubstituted. When substituted, the substituted group (s) is preferably one or more independently selected from the group consisting of alkyl, halo, (halo) 3C-, hydroxy, alkoxy, acyloxy, amino, acylamino, amido, carboxy, carbonyl, alkylcarbonyl, alkoxycarbonyl, cyano and nitro.

An"halo"group refers to fluorine, chlorine, bromine or iodine.

An"hydroxy"group refers to an-OH group.

An"alkoxy"group refers to an-O (alkyl) group.

An"acyloxy"group refers to an-OC (O) (alkyl) group.

An"amino"group refers to an-NRR'group wherein R and R'are independently selected from the group consisting of hydrogen and alkyl, wherein the alkyl group is not further substituted.

An"acylamino"group refers to a-NRC (O) (alkyl) group wherein R is selected from the group consisting of hydrogen and unsubstituted alkyl.

An"amido"group refers to a-C (O) NRR' group wherein R and R'are independently selected from the groups consisting of hydrogen and alkyl, the alkyl group being not further substituted.

A"carboxy"group refers to a-C (O) OH group.

A"carbonyl"group refers to a-C (O) H group.

An"alkylcarbonyl"group refers to a-C (O) (alkyl) group.

An"alkoxycarbonyl"group refers to a-C (O) O (alkyl) group wherein the alkyl group is not further substituted.

A"cyano"group refers to a-C=-N group.

A"nitro"group refers to a-N02 group.

"t-Boc"refers to a t-butoxycarbonyl group; i. e. , (CH3) 3COC (=O)-.

The term"efflux pump"refers to a protein assembly which exports molecules from the cytoplasm or periplasm of a cell to the external environment in an energy dependent fashion.

An"efflux pump inhibitor"is a compound which interferes with the ability of an efflux pump to export molecules from a cell. In particular, the efflux pump inhibitors of this invention interfere with a pump's ability to excrete therapeutic anti-fungal agents from fungal cells or to excrete chemotherapeutic agents from cancerous cells.

By a fungus that"employs an efflux pump resistance mechanism"is meant that the fungal cells are known or are shown to excrete anti-fungal agents from their cytoplasm or periplasm to the external environment and thereby reduce the concentration of the anti-fungal agent in the cells to below that necessary to inhibit the growth and/or proliferation of the cells. Similarly, a mammalian cell that"employs an efflux pump resistance mechanism"is meant that the mammalian cell is known to excrete chemotherapeutic or other pharmaceutical agents to the external environment and thereby reduce the concentration of the agent in the cells to below that necessary to inhibit the growth and/or proliferation of the cells.

In the context of cell growth, the term"inhibit"means that the rate of growth and/or proliferation of a cellular population is decreased, preferably stopped. By "stopped"is preferably meant permanently; that is, the cells are killed. Inhibition can be monitored by, for example, comparing the difference in turbidity of liquid cultures, or the difference in plaque size for cultures on solid media, in the presence and absence of an inhibitory agent.

As used herein, the term"overproduces"refers to the presence in a fungal strain of a significantly greater amount of a functional efflux pump or pumps than that found in most naturally-occurring (usually non-nosocomial) isolates of that strain. A strain that overproduces an efflux pump would, of course, be expected to more efficiently export substrate molecules. In contrast, a"wild-type"strain will produce an efflux pump or pumps at a level that is typical of natural isolates of a particular fungal species. A cancerous cell"overproduces"an efflux pump if it expresses more of the pump than a non-cancerous cell of the same type expresses.

As used herein, the term"anti-fungal agent"refers to a compound that is either fungicidal or fungistatic. A fungicide kills fungal cells while a fungistat slows or stops cell growth and/or proliferation so long as the compound is present. The efflux pump inhibitors of this invention may be somewhat fungicidal or fungistatic in their own right, but their primary utility resides in their ability to potentiate other anti-fungal agents by inhibiting efflux pump activity in resistant fungal strains.

An"azole"anti-fungal agent refers to any member of those classes of anti- fungal agents characterized by one or more imidazole or triazole rings in their chemical structure. Examples, without limitation, of anti-fungal azole compounds are butoconazole, clotrimazole, fenticonazole, ketoconazole, sulfconazole, fluconazole, itraconazole, terconazole, posaconazole, triticonazole, imibenconazole, voriconazole, and metaconazole.

By"potentiation"of an anti-fungal agent is meant that a compound of this invention counteracts the efflux resistance mechanism in a fungal strain sufficiently for an anti-fungal agent to inhibit the growth and/or proliferation of fungal cells at a lower concentration than in the absence of the compound. In cases where resistance is essentially complete, i. e. , an anti-fungal compound has no effect on the fungal cells, potentiation means that, in the presence of a compound of this invention, the anti-fungal agent inhibits the fungus and thereby treats the infection at a pharmaceutically acceptable dosage. Potentiation of a chemotherapeutic agent against a neoplasm or other cancerous cell wold be analogous.

A"sub-inhibitory concentration"of an anti-fungal agent refers to a concentration that is less than that required to inhibit a majority of the cells in a population of a fungal species. Generally, a sub-inhibitory concentration refers to a concentration that is less than the Minimum Inhibitory Concentration (MIC), which is defined, unless specifically stated to be otherwise, as the concentration required to produce an 80% reduction in the growth or proliferation of a target fungus.

As used herein, the term"treat, "treatment,"or"treating"refers to the administration of a therapeutical or prophylactically effective amount of a composition comprising a compound of this invention together with an anti-fungal, chemotherauetic or pharmaceutical agent to a patient in need of such treatment.

As used herein, "infect,"or"infection"refers to the establishment in a patient of a population of a fungus that results in a deleterious effect on the health or well- being of the patient and/or gives rise to discernable symptoms associated with the particular fungus.

A"pharmaceutical composition"refers to a mixture of one or more of the compounds described herein, or physiologically acceptable salts or prodrugs thereof, with other chemical components, such as pharmaceutically acceptable carriers and excipients. The purpose of a pharmaceutical composition is to facilitate administration of a compound to a patient.

A"pharmaceutically acceptable salt"of a compound of this invention refers to the compound in a charged form together with a counter-ion. In general, a compound of this invention will be a positively charged species, usually in the form of an ammonium cation. In such case, the negatively charged counter-ion is a pharmaceutically acceptable anion such as, without limitation, chloride, bromide, iodide, nitrate, phosphate, sulfate, acetate, trifluoroacetate, propionate, butyrate, maleat, fumarate, methanesulfonate, ethanesulfonate, 2-hydroxyethyl-sulfonate, n-propylsulfonate isopropylsulfonate, lactate, malate or citrate. Pharmaceutically acceptable salts in which the compound of this invention forms the positively- charged species are obtained by reacting the compound with the appropriate acid.

For example, to make an ammonium chloride salt of a compound of this invention, the compound would be reacted with hydrochloric acid.

A"prodrug"refers to a compound, which is converted into the parent drug in vivo. Prodrugs are often useful because they may be easier to administer than the parent drug. They may, for instance, be bioavailable by oral administration whereas the parent drug is not. A prodrug may also have improved solubility in pharmaceutical compositions over the parent drug. An example, without limitation, of a prodrug would be a compound which is administered as an ester (the"prodrug") to facilitate transmittal across a hydrophobic cell membrane where water solubility is detrimental. The ester is then metabolically hydrolyzed in the cell to the carboxylic acid, which is the active entity.

A further example of a prodrug would be a short polypeptide such as, without limitation, a 2 to 10 amino acid polypeptide, which is bonded through a terminal amino group to a carboxy group of a compound of this invention. The polypeptide may also bond through a terminal carboxy group with an amino group of a compound herein. The polypeptide hydrolyzes or is metabolized in vivo to release the active molecule.

As used herein, a"pharmaceutically acceptable carrier"refers to a carrier or diluent that does not cause significant irritation to a patient and does not abrogate the biological activity and properties of the administered compound.

An"excipient"refers to an inert substance added to a pharmaceutical composition to further facilitate administration of a compound. Examples, without limitation, of excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils and polyethylene glycols.

As used herein, the terms"prevent", "preventing"and"prevention"refer to a method for barring a patient from acquiring a fungal infection in the first place.

As used herein, the terms"treat", "treating"and"treatment"refer to a method of alleviating or abrogating a fungal infection and/or its attendant symptoms once a patient has been infected. "Treat,""treating,"and"treatment,"when referring to cancer means that, in general, the life expectancy and/or the quality of life of the patient is increased due to administration of the compound herein with an chemotherapeutic agent. With regard to other pharmaceuticals, these terms mean that the subject disease, disorder or infection and/or its attendant symptoms is/are eliminated or ameliorated.

As used herein, "administer,"administering,"or"administration"refers to the delivery to a patient of a compound, salt or prodrug of the present invention or of a pharmaceutical composition containing a compound, salt or prodrug of this invention to a patient for the purpose of inhibiting a fungal efflux pump or a mammalian cell efflux pump. It also refers to the delivery of a composition comprising a compound, salt or prodrug of this invention in combination with an anti-fungal agent, a chemotherapeutic agent or some other pharmaceutical in which case the purpose is the treatment or prevention of a fungal infection, a cancer or some other disease, disorder or infection that the chemotherapeutic or pharmaceutical is known to be useful in the absence of an efflux pump.

The term"patient"refers to any living entity capable of being infected by a fungus, to develop cancer or leukemia or to be subject to some other disease, disorder or infection for which resistance is known to be efflux pump derived. In particular, a"patient"refers to a mammal such as a dog, cat, horse, cow, pig, rabbit, goat or sheep. Most particularly, a patient refers to a human being.

The term"therapeutically effective amount, "as used herein, refers to that amount of a compound of this invention that, together with an anti-fungal agent, a chemotherapeutic or some other pharmaceutical, will relieve to some extent one or more of the symptoms of a fungal infection, a cancer, leukemia or other disease, disorder or infection. In particular, a therapeutically effective amount refers to that amount of a compound of this invention that, together with an anti-fungal agent : (1) reduces, preferably eliminates, the population of fungal cells in the patient's body, (2) inhibits (i. e., slows, preferably stops) proliferation of the fungal cells, (3) inhibits (i. e., slows, preferably stops) spread of the infection, and/or, (4) relieves (preferably, eliminates) one or more symptoms associated with the infection.

The term"prophylactically effective amount"refers to that amount of a compound of this invention and an anti-fungal agent that has the effect of (1) maintaining a reduced level of a population of fungal cells achieved by a previously administered therapeutical effective amount of the compounds; (2) maintaining the level of inhibition of proliferation of fungal cells achieved by administration of a therapeutical effective amount; (3) maintaining the degree of inhibition of spread of the infection achieved by a therapeutically effective amount; and/or (4) maintaining the level of relief of one or more symptoms or, or if symptoms were eliminated, maintaining the non-existence of symptoms associated with a fungal infection achieved by administration of a therapeutically effective amount of the compound of this invention. A prophylactically effective amount also refers to that amount of a composition comprising a compound of this invention and an anti-fungal agent that will prohibit a fungus from accumulating in a susceptible organism in sufficient amount to cause an infection. An example of a susceptible organism would be an immuno-compromised patient such as someone who has undergone transplant surgery and therefore is being treated with immuno-suppressants, or a person suffering from AIDS.

"In vitro"refers to procedures performed in an artificial environment such as, e. g. , without limitation, a test tube or culture medium.

"In vivo"refers to procedures performed within a living organism such as, without limitation, a mouse, rat or rabbit.

A"racemic mixture"refers to a 1: 1 mixture of two optical isomers.

The letters"R"and"S"are used to designate the absolute stereochemistry at an asymmetric carbon. Whether a particular asymmetric carbon atom is R or S is determined by application of the Cahn-ingold-Prelog (C-I-P) rules (Angew. Chem., 1966,78 : 413-447). The rules are so well known to those skilled in the art that they need not be described in detail herein.

Relative stereochemistry refers to the configuration of any asymmetric carbon with respect to any other asymmetric carbon in the same molecule. Unlike absolute configuration, relative configuration is reflection-invariant, that is, it does not affect optical isomerism.

The term"atropisomer"refers to a subclass of conformers (stereoisomers that can be interconverted by rotation about a single bond) that arise as the result of restricted rotation about the single bond. Each conformer represents a potential energy minimum. Thus, each conformer can be isolated as separate chemical species. Reference herein to"a pure atropisomer"refers to a compound wherein greater than 90%, preferably greater than 95%, most preferably greater than 98% of the molecules comprise a single restricted rotation conformer.

A"pure enantiomer"refers to a compound that is greater than 90%, preferably greater than 95% and, most preferably, greater than 98% a single optical isomer.

Discussion The present invention relates to the inhibition of efflux pump activity, particularly in fungal species, and the concurrent potentiation of anti-fungal agents.

It also relates to the inhibition of efflux pump activity in mammalian cells, particularly neoplasms and leukemic cells. The identification and use of efflux pump inhibitors is described in Chamberland et al., Internat. Patent Appl. No. PCT/US96/05469, W096/33285, entitled"Efflux Pump Inhibitors."The following is a description of several efflux pumps that confer resistance to fluconazole on Candida Spp. The description is exemplary only and is not intended to limit the scope of this invention in any manner whatsoever.

Three MDR pumps have been demonstrated to confer resistance to fluconazole in clinical isolates of C. albicans (Sanglard et al., 1996, Antimicrob. Ag.

Chemother. 40: 2300-2305). These pumps are CDR1 (ABC-family, Prasad et al., 1995, Curr. Genet, 27: 320-329), CDR2 (ABC-family, Sanglard et al, 1996, supra) and BenR (MF-family, Benyaakov et al., 1994). The genes which encode CDR1 and CDR2, i. e., cdr1 and cdr2, have been shown to be over-expressed in several C. albicans isolates from AIDS patients with whom fluconazole therapy has failed.

Strains that over-express these genes have also been shown to be resistant to ketoconazole and itraconazole. Over-expression of benR, on the other hand, conferred resistance to fluconazole only. To further explore the resistance mechanism, C. albicans strains were prepared in which the genes expressing individual pumps were deleted. Strains were also produced having multiple gene deletions to further study specificity of the pumps and their role in intrinsic resistance to azole anti-fungals. CDR1 was shown to play a significant role in the intrinsic resistance of C. albicans to azoles in that deletion of the cdr1 gene rendered the strain more susceptible. Deletion of the CDR2 and BenR genes also contributed to intrinsic resistance, but only when cdr1 was also deleted. A C. albicans mutant which was deprived of all known efflux pumps was 30-fold more susceptible to azole anti-fungal agents than the parent strain.

In another study, two homologs of cdr1 and benR, cgcdr and cgben, were cloned from resistant C. glabrata suggesting that a similar resistance mechanism was at work in that species. An active efflux pump in azole-resistant Aspergillus nidulans has also been identified (Waard and van Nistelrooy, 1980, Pesticide Biochem. Physio. 13: 255-266).

Thus, it appears that, in general, strains that are cross-resistant to several anti-fungal azoles tend to over-express CDR1-type broad-spectrum pumps while strains that are resistant only to fluconazole over-express the narrow-spectrum BenR-type pump.

Compounds of this invention are capable of effectively inhibiting several of the above efflux pumps. They may be used to combat both intrinsic and acquired resistance and may in fact expand the spectrum of activity of anti-fungal agents against previously non-susceptible species.

The compounds of this invention are particularly effect in overcoming efflux pump-mediated resistance to azole anti-fungals, expecially fluconazole and posaconazole.

Synthesis The following are General Methods (GM) for the synthesis of the racemic and the enatiomerically enhanced compounds of this invention. Neither the syntheses nor any of the compounds described below are intended, nor are they to be construed, as limiting the scope of this invention in any manner whatsoever. Other approaches to the synthesis of the compounds will become apparent to those skilled in the art based on the disclosures herein and are within the scope of this invention.

The compounds of this invention were characterized by mass spectrometry and their relative retention times (RT) under the following chromatographic protocol : System: HP1100 Zorbax XDB C18 150X3mm, Column: 3.5u Flow rate: 0. 4ml/min UV detn: 240,254nm 0. 1M ammonium acetate, Buffer: pH 6. 0 Organic: acetonitrile Gradien t: % Time (min) % bufferorganic 0 90 10 2 90 10 42 20 80 47 20 80 50 90 10 58 90 10 For compounds that were purified by HPLC, the following protocol was used: Column-Polaris C18, 100 x 21.2 mm Flow rate-20 mL per minute Gradient-0-5 min 10% acetonitrile ; 5-20 min 10% acetonitrile to 100% acetonitrile ; 20-22 min 100% acetonitrile.

GM for N-methyl piperazines (racemic) Compound 213 A solution of anthranilic acid (15 g, 109.4 mmol) in 21 mL (164.1 mmol) of propionic anhydride was stirred at 100 °C for 1.5 hours. The excess propionic anhydride was evaporated (15 torr, 80 °C water bath). The crude mass was co- evaporated three times with toluene to give 18.72 g of A.

1H-NMR (300 MHz, DMSO-d6) 8 : 1.23 (t, J= 7.8 Hz, 3H), 2.67 (q, J= 7.8 Hz, 2H), 7.50-7. 65 (m, 2H), 7.89 (dt, J= 7.6, 1.8 Hz, 1 H), 8.07 (dd, J= 7.6, 1.8 Hz, 1 H).

To a suspension of A (18.72 g 107.0 mmol) in 28 mL of acetic acid was dropwise added 1-amino-4-methylpiperazine (13.48 mL, 112.0 mmol). The mixture was stirred at 90 °C for 14 hours before being evaporated and co-evaporated three times with toluene. The residue was dissolved in water and the pH of the solution was adjusted to 3 by addition of 1 M aqueous hydrochloric acid. The aqueous layer was extracted three times with ethyl ether and the combined organic layers were discarded. The aqueous layer was basified to pH 11 by addition of 2M aqueous sodium hydroxide. After saturation by addition of solid sodium chloride and three extractions with ethyl acetate, the combined organic layers were washed with water, dried over sodium sulfate, filtered through cotton and evaporated in vacuo to give 20.71 g of B.

1H-NMR (300 MHz, DMSO-d6) 8 : 1.23 (t, J= 7.3 Hz, 3H), 2.15 (m, 2H), 2.21 (s, 3H), 2.76 (m, 2H), 2.80-2. 95 (m, 4H), 3.96 (m, 2H), 7.45 (dt, J= 8.1, 1.5 Hz, 1H), 7.58 (dd, J= 8. 1,1. 5 Hz, 1 H), 7.76 (dt, J= 8. 1,1. 5 Hz, 1 H), 8.06 (dd, J= 8. 1,1. 5 Hz, 1 H).

To a solution of B (5 g, 18.4 mmol) and sodium acetate (3.4 g, 41 mmol) in 30 mL of acetic acid was added pyridinium tribromide (11.8 g, 37 mmol). The resulting mixture was stirred at 50 °C for two hours before being cooled to 0 °C. The precipitate was filtered off and washed with acetic acid and hexanes. The resulting powder was suspended in water and the pH was adjusted to 9 by addition at 0 °C of a saturated solution of aqueous potassium carbonate. The white solid was filtered, rinsed with water and dried in vacuo to give 4.2 g of C.

1H-NMR (300 MHz, DMSO-d6) 8 : 2.01 (d, J= 6.6 Hz, 3H), 2.28 (s, 3H), 2.34 (m, 2H), 2.85 (m, 2H), 3.04 (m, 2H), 3.94-4. 03 (m, 2H), 5.72 (q, J= 6.6 Hz, 1 H), 7.55 (dt, J= 8. 1,1. 5 Hz, 1 H), 7.68 (dd, J= 8. 1 Hz, 1. 5Hz, 1H), 7.84 (dt, J= 8. 1,1. 5 Hz, 1H), 8.12 (dd, J=8. 1,1. 5Hz, 1H).

MS (ES+) m/z 351/353 (M++H).

A suspension of C (1.5 g, 4.27 mmol), 2, 4-dimethoxyaniline (0.785 g, 5.12 mmol) and potassium carbonate (0.708 g, 5.12 mmol) in 11 mL of anhydrous dimethylformamide was heated at 85 °C for 4.5 hours. The resulting mixture was diluted with water and extracted three times with a 3/1 (v/v) mixture of ethyl acetate and hexanes. The combined organic layers were washed with water, dried over anhydrous sodium sulfate, filtered, and evaporated to give a dark brown oil which was purified by flash chromatography on silica gel (ethyl acetate/methanol 100/0 to 97.5/2. 5 to 95/5 to 90/10) to give 1.688 g of D.

1H-NMR (300 MHz, CDCI3) 6 : 1.59 (d, J= 6.6 Hz, 3H), 2.26-2. 50 (m, 2H), 2.39 (s, 3H), 2.78-3. 00 (m, 4H), 3.71 (s, 3H), 3.86 (s, 3H), 4.27, 4.34 (2m, 2H), 5.20 (q, J= 6.6 Hz, 1 H), 6.34 (dd, J= 8.7, 2.5 Hz, 1 H), 6.44 (d, J= 2.5 Hz, 1 H), 6.61 (d, J= 8.7 Hz, 1 H), 7.40 (dt, J= 8.1, 1.5 Hz, 1 H), 7.58-7. 74 (m, 2H), 8.20 (dd, J= 8.1, 1.5 Hz, 1 H).

MS (ES+) m/z 424 (M++H).

To a solution of D (15 mg, 0.035 mmol) in 1mL of anhydrous 1,2- dichloroethane was added 4-cyanophenylisocyanate (10 mg, 0.07 mmol). The resulting mixture was heated at 60 °C for 12 hours. Upon removal of solvent, the crude material was purified by HPLC to give 7.0 mg of product.

MS (ES+) m/z 469 (M++H) ; relative retention time 28.0 min.

Table 1 shows additional N-methyl piperazines that were synthesized using the above procedures. The starting materials used to get the different products are shown in the last column.

TABLE 1 Cmpd Structure MS RT (min) Starting materials N, NC anthranilic acid ; 1-amino-4- ,, N 577 methylpiperazine ; 2, 4- NAY , ! Cl (M++H) dimethoxyaniline ; 3- o I i o I i chlorophenyl isocyanate I fut 547/9 4 I N anthranilic acid N c (M++H) . o i i 0 ru ^ N N N'NJ 577/9 6 4 r D (M+-H) anthranilic acid o I oN I (M++H) O, CCN 0 ranz 577/9 .--577/9 7 anthranilic acid 1 (M +H) o/o°/ci J/ zon N N'N 543 8 N. ;'y anthranilic acid Non (MHz zozo P ruz NON N N 547/9 12 N N N + anthranilic acid NYN (M+ +H) 0 j $/No 561 Nay I \ N. NJ 561 13 anthranilic acid NI'N \ (M++I+H) sN, NCNz Zip 0 ranz 557 14 anthranilic acid N (M++H) o/ 0N I 0 ru' 15 N"Y anthranilic acid o, NO. (+) 00 I ? 0 rN' N No /if 18 561/563 561/563 o I i o I i Jo O CNz rA ri 557 9 % o 557 20 ¢ 0NrNsew (M++H) anthranilic acid fY Y YJ (M"+H) ° 1° rf IN N 561 21 N anthranilic acid o o o nN 1 D" fr'N' in 576/578 22 N N Cl anthranilic acid °JaI (M +H) cri N 0P CI I NN vNis 611/613/2-amino-5-chlorobenzoic 23 I- I ogO ° U 615 acid 00 O CNz cl N. N J ci NN 2-amino-5-chlorobenzoic 24 N 577/579 cNNs¢) acid 0 0 U" i Ns cl I j N'NJ 607/609 2-amino-5-chlorobenzoic 25 N 0jaNyN_a (M++H) acid 00 0 0rN' N J 2-amino-5-fluorobenzoic 595/597 38 \ r N acid ; 4-chlorophenyl (MHz "0 00 cl isocyanate Bof I \ N'NJ y 2-amino-5-methylbenzoic 44 N. ; H O, aN YN (M+H+) acid \/o/ ! rut O r N \ N'IN zOo 587 2-amino-5-methoxybenzoic N 45 yN_a (M+H+) acid zozo zu 'NI J N 537 2, 4-difluoroaniline, 62 N-Y H 32. 0 N N (M+H) 4-fluorophenylisocyanate F/IO/F F F " N'NJ 2-amino-6-fluoro-benzoic /579 63 N. 5'y H 27. 8 acid, N N (M+H) 4-fluorophenylisocyanate oleo F ru n i F \ N'NJ 2-amino-4, 5-difluorobenzoic 597 64 F N/H 31. 8 acid, \ N II N \ (M+H) oaN (M+H) 4-fluorophenylisocyanate rN F \ N'N\J 2-amino-4, 5-difluorobenzoic /613/615 65 F/H 35. 1 acid, \ N II N W (M+H) OaN N (M+H) 4-chlorophenylisocyanate 0 0 C) 0 rN' ci NN 596 2-amino-5-chloro-benzoic Li 596 66 eY H 33. 8 acid, TY (M+H) J ; < (M+H) 4-fluorophenylisocyanate 9 ruz F NNl) 579 2-amino-5-fluoro-benzoic LJL 579 67 31. 0 acid, \ N II N \ (M+H) Hz NN ° uF 4-fluorophenylisocyanate o NN'2-amino-5-fluoro-benzoic F NIN 555 acid, 68 zu 34. 0 (M+H) 2, 4-difluoroaniline, F F ° F 4-fluorophenylisocyanate O CNz N-NJ 2-amino-5-methyl-benzoic 591/593 69 N H 34. 8 acid, n<NNsa (M+ H) (M+H) 4-chlorophenylisocyanate ou \ N-NJ 2-amino-5-methyl-benzoic /575 70 N t H 31. 3 acid, H so4O ° uF 4-fluorophenylisocyanate 0 N-NJ 2-amino-5-methyl-benzoic zu 625 71 N H 37. 4 acid, rYTT (M+H) 0, , F 4-trifluorophenylisocyanate I F 0rl"N" "IO_l eN-N--'2-amino-5-methoxy-benzoic "Y 591 73 N 27. 7 acid, 1f (M+H) F 4-fluorophenylisocyanate CN Os N, NJ 2-amino-5-methoxy-benzoic ! j J 607/609 74 uN4,/607/609 32. 8 acid, oj : : (N yN-C (M+H) 4-chlorophenylisocyanate I 0ru' , <NzNC 2-amino-4-chloro-benzoic cy 645/647 75 \ N N \ 38. 1 acid, M+H ) 4-trifluorophenylisocyanate I F F 0N., NNJ 2-amino-4-chloro-benzoic JL, 611/613/6 76 NH 35. 4 acid, O, aNyN_a 15 (M+H) 00 cl 4-chlorophenylisocyanate SOT 0ru O N° XNzNJ 2-amino-4-chloro-benzoic I / 595/597 cW/ 77' H 31. 8 acid, o, aN yN (M+H) s°X1° ° uF 4-fluorophenylisocyanate o I No , ¢ClN/NS 2-amino-4-chloro-benzoic I 591/593 cW v ri 78 ci N"YH 33. 2 acid, O, aN yN (M+H) , [N) (N) CQ (M+H) 4-methylphenylisocyanate I 0r"N' N-NJ 2-amino-4-fluoro-benzoic F w I N 579 81'1 H 30. 2 acid, N N so o ° F 4-fluorophenylisocyanate 0 o CN' 591 2-amino-3-methoxy-benzoic 591 82 o N N N 27. 8 acid, , o N N (M+H) 4-fluorophenylisocyanate O O F lao aF N'NJ 2-amino-3-methoxy-benzoic o 607/609 83 H 31. 2 acid, o0 n. rNNoww (M+H) >1° ° uCI 4-chlorophenylisocyanate 0 ou ace I o eN'1-ethoxycarbonyl-4- 560 aminopiperidine ; following 560 176 N N 28. 3 carbamate hydrolysis with 6N + icc H) HCI, alkylation with dimethyl 0 (D F sulfate 0 IN 214/N/610 4- (difluoromethoxy) phenyl 214 lez H 29. 1 from (M+H) isocyanate o0<0 ° 90d F zip \ N'N595/7 2-amino-4-fluoro-benzoic F /N/59517 215'1 H 34. 0 acid, 3-chlorophenyl NN cl (M+H) "0 o isocyanate 0 r, N, N'N,) 595/7 2-amino-4-fluoro-benzoic F-v-N 216 F N H 33. 5 acid, 4-chlorophenyl erNN>n (M+H) sOJo 1° ° vCI isocyanate s f N N 587 2-amino-4-fluoro-benzoic 217 F N"Y H 30. 2 rNrNon (M+H) acid O wCN o rl--N"2-amino-4-fluoro-benzoic N. nu N'N 628 acid, 4- 218'H 31. 5 sogao o<F (M+H). (difluoromethoxy) phenyl y I F isocyanate O CNz \ N'NJ 2-amino-4-fluoro-benzoic F /N'606 219 N' 28. 2 acid, 5-isocyanato- erNmfNoerO (M+H) °41° ° Wo benzo [1, 3] dioxole I 0N11 F \ N'NJ 2-amino-4, 5-difluoro-benzoic F I N 613/5 220 F NH 35. 7 acid, 3-chlorophenyl o I \ N\/N I \ ci (M+H) ° 1° ° isocyanate O fNz F N 598 2-amino-4, 5-difluoro-benzoic N F-v-N 221 F NH 33. 4 acid, 3-fluorophenyl O I \ N II N I \ F (M-I-H) ° isocyanate O fNo 0 NN 2-amino-4-chloro-benzoic clwN 222 \ N N \ cl 612 37. 6 acid, 3-chlorophenyl fYv'r isocyanate ope \ N-NJ 2-amino-4-chloro-benzoic I / clw\ri 223 NrN F 595 34. 9 acid, 3-fluorophenyl frTYY' isocyanate o N 2-amino-4, 5-difluoro-benzoic FN JLjL acid, 4- 224''tu 645 33. 4 XoJao o ClO<F (difluoromethoxy) phenyl 7 F isocyanate o, r"N'2-amino-4-chloro-benzoic N I s N 643/5 acid, 4- Cri N sOCOY 4so<F (M+H). (difluoromethoxy) phenyl o I i oo I i o F 0 1 F isocyanate fun F \ N>NJ 226 FXN4H 605 31 6 2-amino-4, 5-difluoro-benzoic 226 I \ NN I \ (M+H) 31. 6 acid 00 con I r 11N11 fN /602/4 2-amino-4-chloro-benzoic 227 c N'1'N 33. 3 YTY) (M+H) acid 0-0°"-CN 0 N . .. 2-amino-4-ch ! oro-benzoic 621/3 228 cl N H 31. 1 acid, 5-isocyanato- rYT (M+H) o benzo [1, 3] dioxole GM for other substituted piperazines (racemic) The appropriate N-methyl piperazine compound (1 eq) was dissolved in anhydrous toluene at about 0. 1 M, and a-chloroethyl chloroformate (2.5eq) was added. Heating at 100 C was continued until the reaction was complete (more chloroformate was added if needed, monitoring consumption of starting material by TLC). The solvent was removed in vacuo, and the crude chloroethyl carbamate was dissolved in methanol at a concentration of about 0. 1M. Aqueous HCI (1N ; 1/10 of the volume of methanol) was added and the solution was stirred at room temperature for 1 hour (monitoring by TLC). When no intermediate carbamate remained, the solvent was partially removed in vacuo. The residue was partitioned between ethyl acetate and dilute aq. NaOH (pH<8.5). The organic phase was washed with brine and dried over anhydrous sodium sulfate to give the free piperazine (MS 547 (M+H); RT 26.7 min).

The free piperazine was then reacted with the compounds shown in the last column of Table 2 to give the compounds indicated.

TABLE 2 Cmpd Structure MS RT (min) Reagents P r-A N'N 629 47 N 38. 0 (MHz 0 ( F - I JNJH H Y"- 618 N Y (M+H) O'CN 0N-O'F (M+H) CNXN o",, I-lu N- IV) 744 53 37. 3 N 11'b (M+H) o I q ° I F H N ZON NJ 644 54 NH 27. 7 \ N II N (M+H) , yNvN\J 644 I l NN 0 I N 55'N N \ 705 37. 2 OaN yN (M+H) OO ° F ZON N N 660 57 Ni, H 33 (M+H) ? 0"a'I . ruz 0 N 0- N J 647 di-tert-butyl dicarbonate, 85 42. 2 N NY H (M+H 4-fluorophenylisocyanate non zu 0 0 N'U, 0- N J 86 I/'/65 45. 5 , N, CI (M+H) N\/N I j ci (M H) 0 0 N 0- rf"648 NNJ 648 87 /'43. 8 87 H (M+H) 2, 6-Dimethoxy-pyridin-3-ylamine NyN (M+H) rYYi 0 N ? F N J 629 0 rN j0 88 N :-'y H 42. 3 Phenyl I i te NON (MHZ oo° o e o / 0 ? 647 N. NJ 89 //647 4 3-flu n li N 43. orophe y socyanate NN F (M H) 0 oit 0 N N/\ 623 43. 3 2, 4-difluoroaniline FXF aF F I-F0 F F e Fo/F CNXO 666/6 CI NN J 70/67 2-amino-5-chloro-benzoic acid, 91 wyÆ 45. 3 N N Cl 2 3-chlorophenylisocyanate ° 1° ° (M+H) O N''O^ ci 654/6 92 56 42. 1 2-amino-5-chloro-benzoic acid NERO g HNss (M+H) CNJ0OX N0 NO-\ F N J 665 93 N 43. 6 2-amino-5-fluoro-benzoic acid rYT N'-Y H I i o I 0 F- 649 95 vN'9 H 37. 1 2-amino-3-methoxy-benzoic acid oAo F (M + H) aN T N 0 665/6 2-amino-3-methoxy-benzoic acid, 96 + (<NXf 68 39. 7 "OaNYN,, a (M+H) 4-chlorophenylisocyanate MHz 0,, 0 O CN'Ss N'N, _) 641/6 124 NY H 43 40. 0 methanesulfonyl chloride NYN ci (M+H) 0 0 O I NSO N J 643 2-amino-5-fluoro-benzoic acid, 125 /N 36. 4 (M+H) methanesulfonyl chloride 0 0'laF 0 11 s 0 N 11 607 phenylisocyanate, NN. 126 N (M+H) 34. 9 methanesulfonyl chloride -5 : o I oo I I 0 o. J. So N 626 2, 6-Dimethoxypyridin-3-ylamine, 130 36. 5 N ," (M+H) methanesulfonyl chloride ONO ° F 0 N F 0 rlo 0NCil 673 1-ethoxycarbonyl-4-aminopiperidine, 131 31. 8 aNy (M morpholine I I/11f I/F 0con' , 6 686 1-ethoxycarbonyl-4-aminopiperidine, 132 N 27. 1 N N (M+H) 1-methyl-piperazine 'lu 0II . J JNHz fiv 590 133 /ni 28. 8 ammonia I \ N II N I w (M+H) °Ja 1° X F I zu 0 ZON 674 137 N H 31. 0 morpholine fTT (M+H) 00 F ru 0 on NJ 687 138 N H 27. 8 1-methyl-piperazine \ N II N \ (M+H) 0--0° --F 0 ru 0 N NÆNtJ 675 2, 6-Dimethoxy-pyridin-3-ylamine, 139 N 32. 6 (M+H) morpholine 0 Na' 01 0 roh 0 fNH 0 N, NN AN'N, 673 140 N 25. 4 piperazine b (M+H) zozo o N, N40 N N 659 n 659 143 e,", 28. 5 pyrrolidine rY " o CNt O 0 -N 0 C'N N4D N'NJ 673 145 ° N (M+H) 30. 6 piperidin Non O, any 00) aF 0 r, N NJ 691 146 JCT5 691 thiDmorpholine N N o I o° I/F N 0 d 1'NJ 655 4-imidazoleacetic acid N- 155 N 27. 7 (M+H) hydroxysuccinimide ester \ N \ 00° F o, 743 o zoo O'CNY (M+H) 1-ethoxycarbonyl-4-aminopiperidine, N- 156 1 765 38. 1 t-BOC-D-proline (activated with N- N Y Ny (M+N hydroxy succinimide) i i F a) H H 0 N 645 157 28. 5 2, 6-Dimethoxy-pyridin-3-ylamine Y pY Y (Hz O'N O °'/F HN, 0 H N N/680 158 F 29. 7 2-amino-4, 5-difluorobenzoic acid razz (M+H) OCCN 0"C'F 0 I N''"\ N N N 644 BOC-D-proline N-hydroxysuccinimide "Y 159 \ N (M+H) 27. 0 ester . o I o I i F q 0-0 ° F 0 H H I , NI V 643 160 H 28. 1 1-ethoxycarbonyl-4-aminopiperidine \ N N I \ O/O O/F zou 0rN N 2-amino-4-fluoro-benzoic acid, ,, YN 673 161 673 29. 2 4-imidazoleacetic acid N- T H (M+H) sOJa X ° aF hydroxysuccinimide ester f OUZO F 0 ne=\ N I NNl) 670 1-methyl-4-imidazole acetic acid N- NH N gNNo¢ (M+H) hydroxysuccinimide ester o I ° I F OII I N\/HJO ' N H 719 Y"719 163 e, 36. 9 Boc-beta-alanine \ nus N, N-rol-r j ? 9 Ntot 719 719 164 N 37. 8 Boc-alanine , H OO ° F 0 DN JH 735 165 Nk}/33. 9 Boc-serine non (M+H) % I o F 0 rN'JtN Y07 ( 166 4 I 7 19 37. 8 Boc-sarcosine ine yO'F O\% I o F 0r-\ O I JNO \ NN 0 167 N H 39. 4 Boc-L-beta-homoproline y (MH) 0 ? r o- N 616 - 616 N f. l J'616 173 N 29. 5 2-bromoethyl isocyanate 0NNoes (M + H) s° 1° F O t rN 11 NH2 3-chlorophenylisocyanate, 1 4 08 31. 2 OaNyN,,, N N N I c, (M+H) ammonia o cl 0 o 1'k 1-ethoxycarbonyl-4-aminopiperidine ; I qJk 646 following carbamate hydrolysis with 6N 177 H 42. 8 N N N (M+H) HCI, acylationwithdi-tert-butyl 0-aF dicarbonate 0 0 H Non 644 183 N1/H 27. 2 HPLC separation o Y F NON 0 0 H H !"" ¢NNC) t 644 644 184 N 27. 1 HPLC separation o° 1° F r ! I 0 o J NHZ N 604 604 OaN % r N (M+H) \ N II N \ (M+H) o I o° I F I 0 O N'v'NHZ N J 618 186 26. 0 (M+H) o I i o I 0 r3N NH2 N J 618 f ! rh ! 00° F 0 N 0 rN NH2 O I JNNHz \ N N HO 634 188 NNJHO 634 35. 3 N-'Y H (M+H) C) aN'f N'- F 0 N nu ^NH I \ NN (J 618 o I \ N II N I \ M+H) r O ° F ° ^N'vH NNJ 659 190 N 27. 0 o, any 0"au 0 10 rN H . ( r-ly) N' NJ 701 X AH (M0+1H) 28. 9 formic acid + acetic anhydride Nu soif o i I lo i F ^N N\/0- N'N, _, 0 677 -Y H 32. 6 methyl chloroformate Y (M+H) o I'o° F N ou Ny 0,, 193 N 32. 6 methyl chloroformate 193 N"Y H o I Q I°I I F N N (M+H) Y N, N\J 672 196 N"Y H 33. 6 2-Chloro-1-pyrrolidin-1-yl-propan-1-one Non o I o° I F 0 0 rN ozon N. N J NH 658 197 H 25. 0 I-ly 0 -F N N (M+H) o I i oo I F I I 0 H N N'N 645 2, 6-Dimethoxy-pyridin-3-ylamine ; N V 645 198 /'4 BO-drox succi'ide N H M+H) 26. C D-prolme N-hy y nim NyN (M+H) ester 0-au 0 N r'nu ON Yo 760 38. 9 1- (t-butyloxycarbonyl) piperazine 199 N H N N (M+H) 0ICI 0 N. N J NH 660 200 NNNH 660 25. 0 -'Y N. ; H °, J N1 \ N 662 202 I/' 3. 4 Triphosgene + morpholine sOJWO aF N N O N O v'F 0 O N N- (3-Amino-phenyl)- N'nu 650 204 N/ß/ (M+H) 29. 7 methanesulfonamide ; H' H (M+H) N NN cyclopropanecarbonyl chloride O CNS F 0 . N NI 623 205 N N \ 35. 6 3- [1, 2, 3] Triazol-2-yl-phenylamine AN I/ I/F U N_ Y N /H N'725 2, 4-Bis-cyclopropylmethoxy- NHz /F (M+H) phenylamine 91 cm N O N H. N'NJ N 208 Ny 27. 22 3- [1, 2, 3] Triazol-2-yl-phenylamine N N (M+H) y 0'au ZON 9 r"f"7 N van I/ /602 209 N T H 30. 9 Bromomethyl-cyclopropane NN O/O O/F U 0 r o-jIN-NH, i NtN9NHz 660 210 ° N 24. 7 3- (t-butoxycarbonyl- amino) pyrrolidine (MHz o I o I ° 0 O r"'U, N 0 NN, X CON NEC 674 211 N'y H 24. 9 4- (t-butoxy carbonylamino) piperidine (Hz ? ? 0F 4-fluorophenyl isocyanate ; from free piperazine : p N 632 229 632 33. 0 1. 1, 1'-thiocarbonyldiimidazole, (MHz NOJAfY AF hydrazine, DMF, RT ; CN1H 2. trimethylorthoformate, 90°C NIT N'NI) 576 4-fluorophenyl isocyanate ; N'y 30. 7 Ja Y t (M+H) formic acid + acetic anhydride N sO Ox F 4-fluorophenyl isocyanate ; from free piperazine : 231 659'1. 2- (tert-butoxycarbonylamino)-i- 231 1'0 27. 1 (M+H) cyclopentanecaboxylic acid, NAY EDC, HOBT, DMF, RT ; y "oj ? 2. TFA, dichloromethane J X N'NJ IX"g, 717 From compound with 231, acylating with 232 c', NYm (M+H) methyl chloroformate fifT ! ol ( : x ? 0 F 4-fluorophenyl isocyanate ; from free piperazine : 660 1. triphosgene, triethylamine, 1, 2- 660 233 (M+H) 24. 7 dichloroethane, RT ; N N NHz () ,, y NU N N butoxycarbonylamino) pyrrolidine ; o \ \ F 3. TFA, dichloromethane 4-fluorophenyl isocyanate ; from free piperazine : 1. triphosgene, triethylamine, 1, 2- 718 dichloroethane, RT ; 234 313 fNtNaN°s (M+H) 2. (3-(t- N'0 butoxycarbonylamino) pyrrolidine ; N N 3. TFA, dichloromethane I lfN I 4. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : 1. triphosgene, triethylamine, 1, 2- 718 dichloroethane, RT ; 235 31. 2 N''N."N (M+H) 2. (R)-3- (t- nô, N'NJ o butoxycarbonylamino) pyrrolidine ; NH C-- NYN 3. TFA, dichloromethane . ° ^ ° i F 4. Methyl chloroformate 1 H 0 NN-\.... M 0 N, NJ v °I 754 As compound 235, starting from 2- coma N N (M+H) amino-4, 5-difluorobenzoic acid sO O ° WF 4-fluorophenyl isocyanate ; from free piperazine : 1. triphosgene, triethylamine, 1, 2- 237 718 31. 2 dichloroethane, RT ; 237 o 31. 2 No-ly,, (M+H) 2. (S)-3-(t- N3 butoxycarbonylamino) pyrrolidine ; nez H y y NN 3. TFA, dichloromethane o n 4. Methyl chloroformate 2-amino-5-chloro-benzoic acid ; 4- fluorophenyl isocyanate ; from free 678 8 N,, N piperazine : 238 a. A. J o 28. 9 YYy"1. BOC-L-proiineN- --H (M+H) a Y aF hydroxysuccinimide ester ; o i o 2. 2. TFA O 9> 678/8 °, J 678/8 N N As compound 238, but starting from 2- 239 cl nay 0 29. 5 O, aNyN amino-4-chloro-benzoic acid CNX zon IJN H N'679 As compound 238, but starting from 2- 240 F-N. 29. 5 N (M+H) amino-4-fluoro-benzoic acid 0'au 4-fluorophenyl isocyanate ; from free piperazine : r. NN, 660 24. 5 1 Piperazine-1, 2, 4-tricarboxylic 242 zon H (M+H) acid 1, 4-di-tert-butyl ester, EDC, N"Y H Ja DMF DMF 2. TFA/dichloromethane 4-fluorophenyl isocyanate ; from free CNtN piperazine : 659 243 fi'26. 5 1. Piperidine-1, 2-dicarboxylic acid N, (M+H) Ja N 1-tert-butyl ester, EDC, DMF A o k 2. TFA/dichloromethane 0 0 \\r 0 - J o 0 701 As compound 238, but starting from 2- rN L-/701 244 NN'N 701 32. 2 aminobenzoic acid ; pyrrolidine acylated ils (M+H) with methyl chloroformate 0 ao, As compound 238, but starting from 2- aminobenzoic acid ; pyrrolidine 0rN 245 N 685 subjected to reductive amination with 245 NNJ 33. 3 M+H) U1_ 0X0 ° tF ethoxycyclopropyl) oxy] trimethylsilane OO ° -F and sodium cyanoborohydride As compound 238, but starting from 2- HI o N aminobenzoic acid ; pyrrolidine 659 246 IN 659 27. 0 subjected to reductive amination with MHz NY N paraformaidehyde and sodium QO ° F triacetoxyborohydride 4-fluorophenyl isocyanate ; from free piperazine : NJ 247 NJ (M+H) 32. 4 tetrahydrofuran-2-carboxylic (M+H) soJaI ° uF acid/EDC/DMF q 4-fluorophenyl isocyanate ; from free 0 N NH piperazine : 645 248 N'N 645 25. 4 1. Pyrrolidine-1, 3-dicarboxylic N. 1'H M+H) f acid 1-tert-butyi ester/EDC/DMF OO ° F f 3. TFA/dichloromethane °-703 From compound 248 ; pyrrolidine o-703 249 N"i''32. 5 acylated with methyl chloroformate \ N II N \ (M+H) 0 0 \ N, J s From compound 248 ; pyrrolidine N'N 723 250 N 32. 6 acylated with methane sulfonyl (M+H) y Y chloride/triethyfamine/dichloromethane From compound 248 ; pyrrolidine 752 251 N% s 34. 6 acylated with dimethylsulfamoyl N (M+H) chloride/triethylamine/dichloromethane 4-fluorophenyl isocyanate ; free 657 252 36. 0 piperazine acylated with 5-methyl- . NH (M+H) 0 isoxazole-3-carboxylic acid/EDC/DMF q 4-fluorophenyl isocyanate ; from free N5'H piperazine : 661 253 (Vf'° 27. 6 1. Morpholine-3, 4-dicarboxylic (M+H) acid 4-tert-butyl ester/EDC/DMF o, aNYN, aF : 2 TFA/dichloromethane 4-fluorophenyl isocyanate ; from free 0 piperazine : A Lj 659 254 1 ( (M+H) 26. 0 1. Piperidine-1, 3-dicarboxylic acid --H (M+H) ^/N N\^ 1-tert-butyl ester/EDC/DMF 0 2. TFA/dichloromethane 2-amino-5-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free 1 708/1 R Cr s0 plperazlne : 255 0 \ N'N\J '1. N- (t-BOC) (MHz threonine/EDC/HOBT/DMF o I i o I 2. TFA/dichloromethane 3. 1'1-carbonyldiimidazole/THF H , o ° 708/1 As compound 255, but starting from 2- 256 0 33. 9 0 33. 9 N N amino-4-chloro-benzoic acid ° i o s F (M+H) 0 0 N'r°- 4-fluorophenyl isocyanate ; free 643 257 e,,, 34. 2 piperazine acylated with isoxazole-5- (M+H) H fl Tl carboxytic acid/EDC/DMF 0 r 2-amino-5-chloro-benzoic acid, 4- H N No fluorophenyl isocyanate ; free CI\ ^ jN N 692/4 258 ci N 30. 7 piperazine acylated with L- (MHz SOJAO ° AF pyroglutamic acid/EDC/HOBT/DMF 0" () F N N0 692/4 As compound 258, but starting from 2- 259 ° N 31. 4 N N (M+H) amino-4-chloro-benzoic acid O I Q °I I F 0 F R H H SlN/NCJ R 659 4-fluorophenylisocyanate ; free 659 I 260 Nix 27. 7 piperazine acylated with L-pyroglutamic N N (M+H) YTYY acid/EDC/HOBT/DMF O O F 2-amino-4-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free o 748/5 piperazine : 261 \ N'NJHO^ ° 0 33. 8 4. N- (t-BOC) threonine/EDC/DMF (MHz 0, ( : NyN 5. TFA/dichloromethane 6. Methyl chloroformate 2-amino-4-chloro-benzoic acid, 4- o N'YNF fluorophenyl isocyanate ; from free 778/8 N'J" ^ piperazine : 262 H 0 37. 6 N N N 1. N- (t-BOC)-threonine/EDC/DMF ° 2. TFA/dichloromethane 3. Trifluoroacetic anhydride 2-amino-5-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free , , H.. o 754/6 piperazine : CI. NJ ° N OH (M+H) 1. N- (t-BOC)-threonine/EDC/DMF N N 2. TFA/dichloromethane I I 3. Ethyl chloroformate 2-amino-4-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free H 264, f N o 754/6 piperazine : 264 A J I 352 j OH (M+H) 1. N-(t-BOC)-threonine/EDC/DMF Ci NHz N N 2. TFA/dichloromethane o I w lf I w F 3. Ethyl chloroformate 2-amino-5-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free rNHNyo, 740/2 33. 0 piperazine : 265 ? TY"330 CS N N OHO (M+H) 1. N- (t-BOC)-threonine/EDC/DMF /NH N N 2. TFA/dichloromethane o 3. Methyl chloroformate 2-amino-4-chloro-benzoic acid, 4- fluorophenyl isocyanate ; from free 266 JLH.. o 740/2 piperazine : 266 B 1 Y 336 N"'--OHO (M+H) 1. N- (t-BOC)-threonine/EDC/DMF CI N" N N 2. TFA/dichloromethane 0 3. Methyl chloroformate 2-amino-4-fluoro-benzoic acid, 4- fluorophenyl isocyanate ; from free ol C NXRHNAFON 725 piperazine- 267 0 31. 0 N"HO M+H) 1. N- (t-BOC)-threonine/EDC/DMF N- :' N 2. TFA/dichloromethane 0 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free H 'NNy0,, piperazine : N0 268 I 29. 7 1. N- (t-BOC) threonine/EDC/DMF I N_ N N I \ (M+H) 2. TFA/dichloromethane "oa0y'a', 3. Methyl chloroformate H 2-amino-5-chloro-benzoic acid, 4- I \ NNJ"° ° 782/4 fluorophenyl isocyanate ; from free 269 38. 2 oj (M+H) piperazine : N- (t-BOC)- I N (\ threonine/EDC/DMF , YHN o 2-amino-4-chloro-benzoic acid, 4- N XN NJHo ° 782/4 fluorophenyl isocyanate ; from free 270 CIVN 38. 6 zu CNY DX (M+H) piperazine : N- (t-BOC)- I N I \ ° threonine/EDC/DMF XN O 2-amino-4-fluoro-benzoic acid, 4- Nt i N'N fluorophenyl isocyanate ; from free 271 Fez N N (M+H) piperazine : N- (t-BOC)- threonine/EDC/DMF t HN O 0 N° r4NY01r 4-fluorophenyl isocyanate ; from free 272 N-B 272 NSZ 35. 0 piperazine : N- (t-BOC)- , 'S. H. (M+H) sovf AX S X threonine/EDC/DMF ° H 4-fluorophenyl isocyanate ; from free N i NN, S" piperazine : \ N'NJHO 273 NX 29. 5 1. N- (t-BOC)-threonine/EDC/DMF N (M+H) () \ N N N N 2. TFA/dichloromethane O O ° F 3. Methanesulfonyl chloride/triethylamine/dichloromet hane H NY 4-fluorophenyl isocyanate ; from free an'1 1 785 274 N N 31. 0 piperazine : N- (t-BOC)- M+H)H (p+H) histidine/EDC/DMF 4-fluorophenyl isocyanate ; from free piperazine : 1. 2-tert-Butoxycarbonylamino-4- H 783 275 a> INS (M+H) 32. 0 methanesulfonyl-butyric . . AU LS (M+H) o s acid/EDC/DMF H O NuN 2. TFA/dichloromethane 0-o° F 3. Ethyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : 1. 2-tert-Butoxycarbonylamino-4- 9 H 769 276 O N N° 30. 6 methanesulfonyl-butyric M+H) acid/EDC/DMF nez 2. TFA/dichloromethane o I q° I'F 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : 1. 2-tert-Butoxycarbonylamino-4- 789 methanesulfonyl-butyric 789 277 F 31. 9 acid/EDC/DMF H M+H) 2. TFA/dichloromethane K4NvNJ >o ors 3. Methyl chloroformate, \ N N y difluoroacetic acid, triethylamine, dichloromethane 4-fluorophenyl isocyanate ; from free p N° piperazine : N, N ° 693 278 1 N'HO 28. 8 1. N- (t-BOC)-serine/EDC/DMF (MHz 2. TFA/dichloromethane °I ° i 3. Methyl chloroformate N b ° 4-fluorophenyl isocyanate ; from free 'J 747 piperazine : N- (t-BOC)- 279 40. 7 N (M+H) valine/EDC/DMF N °- I ° 2-amino-4, 5-difluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free nez F')' {669 piperazine : 280 F NN") 28. 5 (M+H) 1. (S)-2-tert-Butoxycarbonylamino- H \o q o N F butyric acid/EDC/DMF O-O ° F 2. TFA/dichloromethane 2-amino-5-chlorobenzoic acid ; 4- fluorophenyl isocyanate ; from free o rN-\ 666/8 piperazine : 281 0l \ N. NJ J 29. 0 N9 (M+H) 1. (S)-2-tert-Butoxycarbonylamino- N"'Y H N N butyric acid/EDC/DMF 0 2. TFA/dichloromethane 2-amino-4-chlorobenzoic acid ; 4- fluorophenyl isocyanate ; from free 0 r'N NH2 666/8 piperazine : 282 1. 0 29. 5 282 N' (M+H) 1. (S)-2-tert-Butoxycarbonylamino- CI NEZ N N butyric acid/EDC/DMF o I \ 2. TFA/dichloromethane 2-amino-4-fluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free 0 fl"N NH, 651 piperazine : 283 A' 27. 4 (M+H) 1. (S)-2-tert-Butoxycarbonylamino- H N N butyric acid/EDC/DMF 0 2. TFA/dichloromethane 4-fluorophenyl isocyanate ; from free 633 piperazine : (lT 633 284 ¢ NC (M+H) 25. 9 1. (S)-2-tert-Butoxycarbonylamino- -"H (M+H)'"' butyric acid/EDC/DMF "aNy _OF 2. TFA/dichloromethane 4-fluorophenyl isocyanate ; from free piperazine : 705 1. (S)-2-tert-Butoxycarbonylamino- 285 runny 35. 2 (M+H) butyric acid/EDC/DMF NHz NN 2. TFA/dichloromethane 3. Ethyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : °, 19 1. (S)-2-tert-Butoxycarbonylamino- 286 iCyjT'36. 7 (M+H) butyric acid/EDC/DMF c.""Y . YYY) 'TFA/dichtoromethane 2. TFA/dichloromethane 3. Isopropyl chloroformate 2-amino-4, 5-difluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free piperazine :, 727 287 N N°, 36. 1 1. 2-tert-Butoxycarbonylamino- (MHz FXN< H butyric acid/EDC/DMF F& N 2. TFA/dichloromethane N \ 3. Methyl chloroformate 2-amino-5-chlorobenzoic acid ; 4- fluorophenyl isocyanate ; from free piperazine : 724/6 288 Neo, 36. 9 1. (S)-2-tert-Butoxycarbonylamino- N. J (M+H) butyric acid/EDC/DMF NAY JaO 2. TFA/dichloromethane 0 3. Methyl chloroformate 2-amino-4-chlorobenzoic acid ; 4- fluorophenyl isocyanate ; from free piperazine : ? w 724/6 (M+H) 37. 4 1. (S)-2-tert-Butoxycarbonylamino- butyric acid/EDC/DMF NU y LOGO F 2. TFA/dichloromethane O-Q ° F 3. Methyl chloroformate 2-amino-4-fluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free piperazine : P H 709 290 NY., 35. 1 1. (S)-2-tert-Butoxycarbonylamino- N Nl-l 0 (M+H) butyric acid/EDC/DMF I H NO, N 2. TFA/dichloromethane 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : NN° 691 1. (S)-2-tert-Butoxycarbonylamino- 291, J & 33. 4 N< H (M+H) butyric acid/EDC/DMF c." N N 2. TFA/dichloromethane OO ° F 3. Methyl chloroformate NY', -' 4-fluorophenyl isocyanate ; from free 733 piperazine : (S)-2-tert- 292 38. 8 (M+H) Butoxycarbonylamino-butyric o I o° ° 0 F acid/EDC/DMF 4-fluorophenyl isocyanate ; from free piperazine : N. NJ 0 719 . k., N 0/jU 293 I 37. 0 1. N- (t-BOC)-valine/EDC/DMF (Hz 2. TFA/dichloromethane y 1° F 3. Ethyl chloroformate 4-fluorophenyl isocyanate ; from free '° piperazine : JL A'733 294 NF H (M+H) 38. 3 1. N- (t-BOC)-valine/EDC/DMF I N, N N I (M+H) 2. TFA/dichloromethane 0 °l 3. Isopropyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : N. N 0 705 295 NSX 34. 8 1. N- (t-BOC)-valine/EDC/DMF (M H () 2. TFA/dichloromethane O ° -F o o ° F 3. Methyl chloroformate 2-amino-4, 5-difluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free CNX, N) CON 713 piperazine : 296 F>4N/NJ É O 34 9 ' (M+H) 34. 9 1. N- (t-BOC)- (D)-alanine/EDC/DMF I H N N 2. TFA/dichloromethane 0-p° -F 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : N. NJ-° 677 297 NSW H 32. 1 1. N- (t-BOC)- (D)-alanine/EDC/DMF N H M+H) 2. TFA/dichloromethane O O F 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free 0 j piperazine : 705 1. N- (t-BOC)-N-methyl- 298-Y H (M+H) atanine/EDC/DMF o I °° I F 2. TFA/dichloromethane 3. Ethyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : rN Nyo-r 719 1. N- (t-BOC)-N-methyl- 299 N-NJ ° 37. 1 (M+H) alanine/EDC/DMF nay 2. TFA/dichloromethane 3. Isopropyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : 300 rNN--rO-691 1. N- (t-BOC)-N-methyl- 300 JN' 33. 9 N/9 H (M+H) alanine/EDC/DMF N I H \0 Y aF 2. TFA/dichloromethane . o I i [oi I F ! 3. Methy) ch) oroformate .. JNO N, J o 4-fluorophenyl isocyanate ; free '733 N 39. 0 piperazine acylated with N- (t-BOC)- (M+H) p p Y () , F N-methyl-alanine/EDC/DMF 0'ouf 2-amino-4, 5-difluorobenzoic acid ; 4- NHz , N fluorophenyl isocyanate ; from free NN,-"655 302 I N 655 27. 7 piperazine : F nah fY"Y"' 1. N- (t-BOC)-aianine/EDC/DMF OO ° F 1° 2. TFA/dichloromethane 0 r'N NH, N. J 652/4 As compound 302, but starting from 2- 303/N 27. 9 N N (M+H) amino-5-chlorobenzoic acid , aNyN_C_, 00 F r'N'If NH2 1 0 NN,--, 652/4 As compound 302, but starting from 2- 304 Cl NX 28. 4 N HN (M+H) amino-4-chlorobenzoic acid 0--0° F 0 0 IT NH, O CN NH2 637 26. 3 As compound 302, but starting from 2- 305 F NH 26. 3 N (M+H) amino-4-fluorobenzoic acid o e p I e F O o F 0--0° F . N u. 0 713 34. 8 As compound 302, but using the (L)- 306 F 34. 8 N N (M+H) alanine derived reagent O I'Q ° I F N Nu°w CI N NO o 710/2 As compound 306, but starting from 2- 307 zu 35. 6 (M+H) amino-5-chlorobenzoic acid 0' (Y O'F 0 O I NN II 0 "0 N'_, 0 707 As compound 306, but starting from 2- 308 N/zAZ 32. 6 N"N (M+H) amino-5-methoxybenzoic acid O'0° Lp I N N N 0 °w 'J 710/2 As compound 306, but starting from 2- 309 36. 0 N N (M+H) amino-4-chlorobenzoic acid O O F c rY N O 695 As compound 306, but starting from 2- 310 F 33. 5 (M+H) amino-4-fluorobenzoic acid 00° F t HN o 2-amino-4, 5-difluorobenzoic acid ; 4- F r-NN y 0, _r 2 ,,, N, _) 0 755 fluorophenyl isocyanate ; from free 311 Fez N N (M+H) piperazine : N- (t-BOC)- o I 4° I'F °41° F alanine/EDC/DMF C NYY ci"I N'NJ 0 xi, 752/4 As compound 311, but starting from 2- 312 N'Y H 41. 0 312 \nJo, (M+H) amino-5-chlorobenzoic acid 0 ? r 4-fluorophenyl isocyanate ; from free N , piperazine : N'N, _, 647 313 e N 647 26. 9 1. 2-chloropropionyl chloride, N"YH M+H) N trieth lamine dichloromethane y 2. dimethylamine, ethanol 2-amino-4-chlorobenzoic acid ; 4- fluorophenyl isocyanate ; from free °If piperazine : 692/4 314 H (M 29. 6 1. (R)-2-carboxymethyl-pyrrolidine- N (M H) -carboxylic acid ter-butyl ester N' N N d/EDC/DMF 0 2. TFA/dichloromethane 2-amino-4, 5-difluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free piperazine : 0 NN 695 315 F H H 28. 6 1. (S)-2-carboxymethyl-pyrrolidine- N-N--) (M+H) F 1-carboxylic acid ter-butyl ester N d/EDC/DMF \o I NuN I % F dIEDC/DMF 2. TFA/dichloromethane 'N'14CN 0 NNJ 6g2/4 As compound 315, but starting from 2- 316 N 29. 0 (M+H) amino-5-chlorobenzoic acid OO ° F 0'aNYN' 0 au A N H NN,-) 692/4 As compound 315, but starting from 2- 317 ci 29. 6 (M+H) amino-5-chlorobenzoic acid 0 ? 0 ,/ l-NHZ I,-NH2 4-fluorophenyl isocyanate ; from free N NJ 688 318 30. 0 piperazine : (2-amino-thiazol-4-yl)- T M (M+H) acetic acid/EDC/DMF ? 0'aF 4-fluorophenyl isocyanate ; from free o piperazine : H 319 1 IN 691 32. 6 1. N- (t-BOC)-p-alanine/EDC/DMF H (M+H) 2. TFA/dichloromethane 0 3. Ethyl chloroformate 4-fluorophenyl isocyanate ; from free O CNXNXOX 705 piperazine : H 320 « (M+H) 34. 2 1. N- (t-BOC)-p-alanine/EDC/DMF e,"Y H N N C) 2. TFA/dichloromethane 0 3. Isopropyl chloroformate 4-fluorophenyl isocyanate ; from free O CNt HtO-piperazine : N H 677 321 I N-NJ 677 31. 0 1. N- (t-BOC)-p-alanine/EDC/DMF N N N 2. TFA/dichloromethane . o I i o I i F 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : R No N- (t-BOC)-SAR-OH/EDC/DMF 322 MN NJ O 33. 6 (M+H) 2. TFA/dichloromethane N H o, aNYN 3. Ethyl chloroformate 0'las 4-fluorophenyl isocyanate ; from free NN 0 r'l y piperazine : NNJ 0 705 323 35. 3 1. N- (t-BOC)-SAR-OH/EDC/DMF N H (M+H) J Nla, 2. TFA/dichloromethane . o I i ° F 3. Isopropyl chloroformate ) 4HN O 4-fluorophenyl isocyanate ; from free N ° 663 piperazine : 324 N"Y H 324'aNYNH (M+H) 31. 0 1. N-(t-BOC)-glycine/EDC/DM F 2. TFA/dichloromethane 3. Methyl chloroformate 4-fluorophenyl isocyanate ; from free piperazine : 1. N- (t-BOC)-glycine/EDC/DMF 683 325 d A, N-S (M8+3H) 31. 0 2. TFA/dichloromethane N/SX 3. Methanesulfonyl , aN N>Ct chloride/triethylamine/dichloromet hante 2-amino-4, 5-difluorobenzoic acid ; 4- fluorophenyl isocyanate ; from free Q I N/\NHZ 669 piperazine : 326 \ N 28. 1 (M+H) 1. 2-tert-butoxycarbonylamino-2 F N H a NyN methyl-propionic acid/EDC/DMF Op ° F 2. TFA/dichloromethane CNiNH2 ci N'N 666/8 As compound 326, but starting from 2- ' N H 327 WNX HN (M+H) 28. 4 amino-5-chlorobenzoic acid o I ° q° I ° F I N/\NHz CN9NH2 N 666/8 As compound 326, but starting from 2- 328 cl 1 29. 0 328, ¢ NNs (M+H) 29. 0 amino-4-chlorobenzoic acid O O ° F l 0 N N 651 As compound 326, but starting from 2- 329 F 26. 9 (M+H) amino-4-fluorobenzoic acid o ? F I N f \NHz N'J 633 As compound 326, but starting from 2- 330 N' 25. 5 NN ^ (M+H) aminobenzoic acid 0 F H N zip F N N'J 705 From compound 330, acylating with N''Y H N N (M+H) ethyl chloroformate o I NN N/\Npo I N N 0 719 From compound 330, acylating with 332 c."34. 4 N (M+H) isopropyl chloroformate o ? F N Nu°w N'N J I°I 727 From compound 326, acylating with 333 F 34. 3 (M+H) methyl chloroformate o 0-caf °'I \ N'NJ I°'724/6 As for compound 333, but starting from 334 nix 34. 9 (M+H) 2-amino-5-chlorobenzoic acid o o N N O N'J 72416 As for compound 333, but starting from 335 35. 3 N N (M+H) 2-amino-4-chlorobenzoic acid o ? F \ N N N°o rY , N0N Y 709 As for compound 333, but starting from 33. 0 336 (M+H) 2-amino-4-fluorobenzoic acid 0 ? I 0 \ N. NJN/\N 0 Ow 691 As for compound 333, but starting from NH \ N\/N \ (M+H) 2-aminobenzoic acid o S F 2-amino-4, 5-difluorobenzoic acid ; 4- H N'JNN o ° 769 fluorophenyl isocyanate ; from free 338 F I N 'I 38. 8 piperazine : 2-tert- - 1'" (M+H) sog) ? uF butoxycarbonylamino-2-methyl- propionic acid/EDC/DMF N N-NJ ° 766/8 As for compound 338, but starting from 339 39. 7 (M+H) 2-amino-5-chloro-benzoic acid 0 1° wF N9NH o 'J 766/8 As for compound 338, but starting from 340 Cl 40. 2 (M+H) 2-amino-4-chloro-benzoic acid O IO F d NS nu 0 733 As for compound 338, but starting from 341 N N N \ 36. 1 , NN (M+H) 2-aminobenzoic acid 0 F 3- [1, 2, 3] triazol-2-yl-phenylamine ; 4- fluorophenyl isocyanate ; from free piperazine : 725 1. triphosgene, triethylamine, 1, 2- 725 342 o 31. 5 dichloroethane, RT ; o N A N>N O (M + H) 2. 3- (t- /O (. 1 1 butoxycarbonylamino) pyrrolidine ; X F 3. TFA, dichloromethane NL AN 4. methyl chloroformate - N'N,) 0\ 798 As for compound 342, but replacing 3- 343 tj 38. 6 [1, 2, 3] triazol-2-yl-phenylamine with 2, 4- M+H V » ° 9 F dicyclopropoxy-phenylamine 7 2, 6-dimethoxy-pyridin-3-ylamine ; 4- fluorophenyl isocyanate ; from free piperazine : o 661 1. triphosgene, triethylamine, 1, 2- 344. . -\. u 25. 7 344 0'NNNHZ (M+H) 25. 7 dichloroethane, RT ; N__) 2. 3- (t- N N butoxycarbonylamino) pyrrolidine ; ON-O ° F 3. TFA, dichloromethane 0 N As for compound 238, starting from 2- OH 655 aminobenzoic acid and replacing 2, 4- N2 CO ° UF (M+H) dimethoxyaniline with 2- I o° I/F cyclopropoxyaniline N As for compound 238, starting from 2- j) Jty N N 655 aminobenzoic acid and replacing 2, 4- 346 N- 30. 7 NUS N (M+H) dimethoxyaniline with 4- cyclopropoxyaniline 0 O'N'Y"NZ As for compound 302, starting from 2- N 347 Ng N 699 32. 0 aminobenzoic acid and replacing 2, 4- 347'jj 32. 0 Y (M+H) dimethoxyaniline with 2, 4- dicyclopropoxy-phenylamine V o rN, As for compound 306, starting from 2- I N"J'757 aminobenzoic acid and replacing 2, 4- 348 39. 3 (M+H) dimethoxyaniline with 2, 4- O O F dicyclopropoxy-phenylamine 0 d sN NyOu As for compound 306, starting from 2- 0 684 aminobenzoic acid and replacing 2, 4- 349 N a \ 33. 4 I I (M+H) dimethoxyaniline with 3- [1, 2, 3] triazol-2- F JL yi-phenyiamine NJ As for compound 229, replacing 2, 4- N dimethoxyaniline with 4-fluoro-2- ) ! ! P 604 350 N'/H 34. 9 methoxyaniline and acylating the free (M+H) I % N o N I % piperazine with cyclopropane carbonyl ro °'F chloride 0 N-'-v N As for com ound 350, replacing 2, 4- /634 p p g 351 I N T H 37. 2 dimethoxyaniline with 2- I/N O N I (M+H) o F trifluoromethoxyaniline F4F O 0 N-7 N'N As for compound 350, replacing 2, 4- 626 352 b \ M+H 37. 6 dimethoxyaniline with 2- Y Y (M+H) 0 F cyclopropoxyaniline 0 N 600 As for compound 350, replacing 2, 4- 353 a 35. 7 (M+H) dimethoxyaniline with 2-ethoxyaniline OK WF GM for enantiomerically enriched compounds A mixture of L-Lactic acid (1,85% solution in water; 23.5 ml), glacial acetic (89ml), benzene (18ml) and concentrated sulfuric acid (0. 05ml) were heated at reflux with the continuous removal of water. After 24 hours the mixture was neutralized with sodium acetate (220mg) and distilled in vacuo (100 *C/I mm Hg) to give 19.94 gm of 2.

Compound 2 (19.94 gm) was heated with thionyl chloride (12 ml) gradually for two hours to a final temperature of 95 C, followed by distillation in vacuo (50 °C/5mm Hg) to give 18. 17 gm of 3.

Anthranilic acid (11.0 gm, 0.080 mol) in dry toluene (400mi) was cooled to 0 C. Triethylamine (33.64 ml, 0.24 mol) was added. A solution of 3 (18.17 gm, 0.121 mol) in toluene (1 Oml) was added slowly to the reaction mixture over 30 min. After stirring at room temperature for 1 hour, the resulting precipitate was removed by filtration and the filtrate was concentrated in vacuo. The residue was taken up in dichloromethane (300 ml), washed with water (50 ml), 1 M HCI (50 ml), and brine (50 ml), dried over anhydrous magnesium sulfate, and concentrated in vacuo to give a 19.98 gm of a mixture of 4 and 5.

The mixture of 4 and 5 (19.98 gm) was heated with acetic anhydride (100ml) at 95 C for 2 hours. The solvent was removed in vacuo. Toluene (10 mi) was added and the mixture was again concentrated in vacuo to give 19.45 gm of product.

A solution of the above product (19.45 gm, 0.083 mol) in acetic acid (50 ml) was cooled to 0 °C. 1-Amino-4-methylpiperazine (10 ml, 0.083 mol) was added over 15 minutes. The reaction mixture was heated at 90 °C for 18 hours and then cooled to room temperature. The solvent was removed in vacuo. Toluene (20 mi) was added and the mixture was again concentrated in vacuo. The residue was diluted with water (25 ml) and the pH adjusted to 3 with 1 M HCI. The aqueous phase was extracted with ether (2 X 30 ml) and ether extract was discarded. The aqueous phase was then cooled in an ice bath and the pH was adjusted to 11 with 2M NaOH.

This was then extracted with dichloromethane (2 X 40 ml), and the combined extracts were washed with brine (30 ml), dried over anhydrous magnesium sulfate and concentrated in vacuo to give 17.96 gm of a mixture of 6 and 7.

A solution of the above mixture of 6 and 7 (17.96 gm) in methanol (200 ml) was treated with aqueous potassium carbonate (20 gm in 100 ml of water), and the mixture was stirred at room temperature for 1 hour. The solvents were evaporated, and the residue was extracted with dichloromethane (3 X 30 ml). The combined extracts were washed with brine (30 ml), dried over anhydrous magnesium sulfate, and concentrated in vacuo. The residue was purified by flash chromatography on silica gel, eluting with 3% MeOH/dichloromethane to give 15 gm of 7.

A solution of 7 (15 gm, 0.052 mol), triphenylphosphine (27.27 gm, 0.104 mol) and benzoic acid (12.7 gm, 0.104 mol) in dry THF (250 ml) was cooled to 0 °C.

Diisopropyl azodicarboxylate (21 gm, 0.104 mol) was added dropwise over 30 minutes, the mixture was allowed to warm to room temperature and the it was stirred overnight. The solvent was evaporated and the residue was dissolved in dichloromethane (300 ml), washed with 10% sodium carbonate (100 ml) and brine (100 ml), dried over anhydrous magnesium sulfate and concentrated in vacuo. The residue was purified by flash chromatography on silica gel, eluting with 4% MeOH/dichloromethane to give 15 gm of 8.

A solution of 8 (15 gm) in methanol (200ml) was treated with aqueous potassium carbonate (17 gm in 100ml of water), and the mixture was stirred at room temperature for 18 hours. The solvents were evaporated, and the residue was extracted with dichloromethane (3 X 50 ml). The combined extracts were washed with brine (50 ml), dried over anhydrous magnesium sulfate and concentrated in vacuo. The residue was purified by flash chromatography on silica gel, eluting with 5% MeOH/dichloromethane. to give 10 gm of 9.

A solution of 2, 4-dimethoxyaniline (1. 0gm, 0.00653 mol) in dry dichloromethane (50ml) was added dry pyridine (0.516 gm, 0.00653 mol, 1 eq) and 2, 4-dinitrobenzenesulfonylchloride (1.74 gm, 0.00653 mol, 1 eq). The mixture was stirred at room temperature for 2 hours, washed with water (20 ml), 10% aqueous potassium carbonate (20 ml), and brine (20 ml), dried over anhydrous MgS04 and concentrated in vacuo. The residue was purified by flash chromatography on silica gel, eluting with dichloromethane to give 1.7 gm of 10.

To a solution of 9 (10 gm, 0.034 mol), and triphenylphosphine (17.83 gm, 0.068 mol) and 10 (26.0 gm, 0.068 mol) in dry THF (1100 mi) was added diisopropyl azodicarboxylate (13.75 gm, 0.068 mol), dropwise over 30 minutes, at room temperature. The resulting mixture was stirred overnight, and concentrated in vacuo. The residue was dissolved in dichloromethane (1500 ml), washed with 10% sodium carbonate (400 ml) and brine (400 ml), dried over anhydrous magnesium sulfate and partially concentrated in vacuo. The precipitate that formed was isolated by filtration to give 10, which can be reused. The filtrate was concentrated and the residue purified by flash chromatography on silica gel, eluting with 6% MeOH/ethyl acetate to give 19.5 gm of 11.

To a solution of 11 (19.5 gm, 0.029 mol) in dichloromethane (500 ml) was added triethylamine (8.06 ml, 0.058 mol) and mercaptoacetic acid (6.73 ml, 0.087 mol) at room temperature. After stirring for 3 hours, the solution was diluted with dichloromethane (500 ml) and washed with 10% potassium carbonate (200 ml) and brine (200 ml), dried over anhydrous magnesium sulfate, and concentrated in vacuo.

The residue was purified by flash chromatography on silica gel, eluting with 6% MeOH/ethyl acetate to give 12 gm of 12.

To a solution of 12 (12.0 gm, 0.028 mol) in dichloromethane (200 ml) was added 4-fluorophenyl isocyanate (5.82 gm, 0.042 mol), and the mixture was stirred at room temperature for 18 hours. The precipitate that formed was filtered and the filtrate was concentrated in vacuo. The residue was purified by flash chromatography on silica gel, eluting with 3% MeOH/dichloromethane to give 14.5 gm of 13.

To a solution of 13 (400mg, 0.672 mmol) in anhydrous chloroform (20mL) was added diisopropylethylamine (585 uL, 3.36 mmol, 5 equivalents) followed by 1- chloroethyl chloroformate (290uL, 2.69 mmol, 4 equivalents). The mixture was refluxed for 1 hour, cooled to room temperature and concentrated in vacuo. The residue was dissolved in ethyl acetate and washed with brine. The aqueous layer was extracted with ethyl acetate and the combined organic phases were dried over sodium sulfate and concentrated to give a brown solid, which was then suspended in MeOH (20mi) and treated with 6N HCI (20 drops). The mixture was heated at 65 °C for 10 minutes, whereupon it became homogeneous. After cooling to room temperature, the reaction was quenched by the addition of a saturated aqueous sodium bicarbonate until the solution was basic (pH = 8 to 9). After extraction with ethyl acetate (3 x 100mL), the combined organic layers were dried over anhydrous sodium sulfate and concentrated to dryness to give 200 mg 14 as a brown solid.

Compound 14 was then reacted with the appropriate reagents to give the compounds of this invention shown in Table 3.

Methods for the stereoselective synthesis ab initio of other compounds of this invention will become apparent to those skilled in the art based on the disclosures herein. Such enantiomerically pure compounds are within the scope of this invention.

TABLE 3 Reagents, starting Cmpd Structure MS RT (min) Reagents, starting materials 0 2-amino-4- IN fluorobenzoic acid ; 354 F NH 580 (M+H) 29. 9 N0N 4-fluorophenyl y isocyanate ZON 0ru razz 355 H 632 (M+H) 32. 9 See compound 229 \ NuN \ NON 0 y'a,, H N O ozon 356 uN 703 (M+H) 32. 1 See compound 244 \ NN \ OaN yo" 0 N J H 'non 357 Cl 678/80 (M+H) 29. 6 See compound 239 H 357 NH N soft xi, yin O Ny0,, 358 N 740/2 (M+H) 32. 9 See compound 265 NH 358 \ NuN \ NON0 \ N j N l N ° ° 359 kN O 740/2 (M+H) 33. 2 See compound 266 \ NuN \ . ; T dN SHy \ N'NJHO 0 See compound 272 H 0 N'fN O I \ N I'N I \ F zu \ N N o 361 N 691 (M+H) 33. 6 See compound 291 \ NuN \ 0 I NNHx . nu 362 s g0 652/4 (M+H) 28. 5 See compound 304 0 1° tF NUS ol'qll I°F N NuW F \ N. N J IIO 363 F N N 713 (M+H) 34. 5 See compound 306 z H sO O F 9 r-y\Y- /N% NUW ci 9NX Y 364 N 710/2 (M+H) 35. 6 See compound 307 Nu osa ? 0 F " FN NJ As compound 364, but \ N- 365 HN 695 (M+H) 32. 9 starting from 2-amino-5- 365 NH so o ° F fluorobenzoic acid NC X Y N'Nj 0 366 aN' 710/2 (M+H) 36. 0 See compound 309 N ! oh ° 1° F O I \ N II N I \ F . \ N N 367 F&"695 (M+H) 33. 0 See compound 310 H () p Nus \ 0 I'O ICI I ° F i S N Y As compound 364, but r 368 4YNgs 677 (M+H) 32. 0 starting from 2- H \ tJUN \ O O ° F aminobenzoic acid N NH= _ Ci 369 a 666/8 (M+H) 28. 0 See compound 327 \ NHz N Ny0, As compound 337, but 370 H 709 (M+H) 32. 3 starting from 2-amino-5- na H .-q o i F fluorobenzoic acid S ravi r y 724/6 (M+H) 35. 0 See compound 335 sO -F 'F N N N ow - J 372 F N-H 709 (M+H) 33. 7 See compound 336 s NN 0 Y-OF I o N N N II W 373 H 691 (M+H) 31. 5 See compound 337 "aN 0N \ NuN \ O I'O II I'F Pharmaceutical compositions and modes of administration An efflux pump inhibitory compound, or salt of prodrug thereof, and an anti- fungal agent may be administered to a patient serially or simultaneously. If serial administration is contemplated, the presently preferred approach is to administer the compound of this invention first. This permits the compound to inhibit the efflux pump (s) of the target fungal cells before the anti-fungal agent is administered, which should result in a substantially lower dosage of the anti-fungal agent being required since the fungal cells will not be able to excrete the agent. By"simultaneous" administration is meant that a compound of this invention and an anti-fungal agent are administered to a patient at essentially the same time. This can be accomplished by administering the compound herein and the anti-fungal agent separately, as in the case of two separate tablets or capsules, separate I. V. drips, or separate injections administered one immediately after the other, which, as used herein, constitutes"simultaneously."In a presently preferred embodiment, "simultaneously"means that the compound of this invention is prepared as a homogeneous composition with an anti-fungal agent and that composition is administered to the patient. In the alternative, a compound of this invention may be administered to a patient first and then, after it has had the opportunity to inhibit the efflux pump of the fungicidal cells, the anti-fungal agent is administered. Since the fungal cells will no longer be able to excrete the anti-fungal agent via the effluix pump, the agent will accumulate in the cells in sufficient concentration to inhibit the cells and treat the infection.

A compound of the present invention, a prodrug thereof or a physiologically acceptable salt of either the compound or its prodrug, can be administered as such to a patient or as a pharmaceutical compositions in which the compounds are mixed with suitable carriers or excipient (s). Techniques for formulation and administration of drugs may be found in Remington's Pharmacological Sciences, Mack Publishing Co. , Easton, PA, latest edition.

Suitable routes of administration include, without limitation, oral, rectal, vaginal, transmucosal, intramuscular, subcutaneous, intramedullary, intrathecal, direct intraventricular, intravenous, intravitreal, intraperitoneal, intranasal, or intraocular. The presently preferred routes of administration are oral and parenteral.

Alternatively, one may administer the compound in a localized topical rather than systemic manner. That is, the homogeneous composition of a compound herein and an anti-fungal agent can be applied directly to the surface of an infected area or injected directly into the infection.

Pharmaceutical compositions of the present invention may be manufactured by processes well known in the art, e. g. , by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or lyophilizing processes.

Such pharmaceutical compositions are formulated in conventional manner and may include one or more pharmaceutically acceptable carriers, excipients and/or auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutical. Proper formulation is dependent upon the route of administration chosen.

For injection, the compounds of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks'solution, Ringer's solution, or physiological saline buffer.

For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.

For oral administration, the compounds can be formulated by combining the active ingredients with pharmaceutical acceptable carriers well known in the art.

Such carriers enable the compounds of the invention to be formulated as tablets, pills, lozenges, dragees, capsules, liquids, gels, syrups, slurries, suspensions and the like, suitable for oral ingestion. Pharmaceutical preparations for oral use can be made using a solid excipient, optionally grinding the resulting mixture, and processing the mixture of granules, after adding other suitable auxiliaries if desired, to obtain tablets or dragee cores. Useful excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol, cellulose preparations such as, for example, maize starch, wheat starch, rice starch and potato starch and other materials such as gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone (PVP). Disintegrating agents may also be added, such as cross-linked polyvinyl pyrrolidone, agar, or alginic acid. A salt such as sodium alginate may also be used.

Dragee cores are often provided with suitable coatings. For this purpose, concentrated sugar solutions may be used which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings to identify the particular compounds in that composition and/or their dosages.

Pharmaceutical formulations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with a filler such as lactose, a binder such as starch, and/or a lubricant such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. Stabilizers may be added in these formulations, also.

For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray using a pressurized pack or a nebulizer and a suitable propellant, e. g. , without limitation, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane or carbon dioxide. In the case of a pressurized aerosol, the dosage may be controlled by providing a valve that delivers a metered amount. Capsules and cartridges of, for example, gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

The compounds may also be formulated for parenteral administration.

Formulations for parenteral injection may be in unit dosage form, e. g. , in single-dose ampoules, or in multi-dose containers. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulating materials such as suspending, stabilizing and/or dispersing agents.

Pharmaceutical compositions for parenteral administration include aqueous solutions of a water soluble form, such as, without limitation, a salt, of the active compound. Or, suspensions of the active compounds may be prepared in a lipophilic vehicle. Suitable lipophilic vehicles include fatty oils such as sesame oil, synthetic fatty acid esters such as ethyl oleate and triglycerides, or materials such as liposoms. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers and/or agents that increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.

Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e. g., sterile, pyrogen-free water.

The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, using, e. g., conventional suppository bases such as cocoa butter or other glycerides.

The compounds may also be formulated as depot preparations. Such long acting formulations may be administered by implantation (for example, subcutaneously or intramuscularly) or by intramuscular injection. A compound of this invention may be formulated for this route of administration with suitable hydrophobic materials (for instance, in an emulsion with a pharmacologically acceptable oil), with ion exchange resins, or as a sparingly soluble derivative such as, without limitation, a sparingly soluble salt.

Delivery systems for hydrophobic pharmaceutical compounds include, without limitation, liposoms and emulsions. These are well known examples of delivery vehicles or carriers for hydrophobic drugs. In addition, certain organic solvents such as dimethylsulfoxide may be employed, although often at the cost of greater toxicity.

The compounds may be delivered using a sustained-release system, such as semipermeable matrices of solid hydrophobic polymers. Sustained-release materials and methods are well known to those skilled in the art. Sustained-release capsules may, depending on their chemical nature, release the compounds for a relatively short period of time, a few days perhaps even a few hours, or over very long periods of time such as 100 days or more.

The pharmaceutical compositions herein may comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include, but are not limited to, calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.

Dosage Determination of a dosage that will result in a therapeutically effective amount of a fungal agent and compound of this invention being delivered to a patient will require assessment of such parameters as, without limitation, the age, gender, weight and physical condition of the patient as well as the severity of the infection, route of administration and response to previous treatments, if any. All of these are well within the knowledge and expertise of the treating physician.

In addition to the above considerations, it will be understood that the maximum permissible dose of known anti-fungal agents can be readily found in the pharmacological literature. The effect of various quantities of a compound of this invention on the amount of two conventional anti-fungal agents required to treat a fungal infection are disclosed herein. If other anti-fungal agents and/or other compounds are selected for use, the effect of various quantities of the compound on the efficacy of the anti-fungal agent can be determined without undue experimentation using the methods described herein.

While it may on occasion be desirable, even necessary, to treat a patient with massive doses of an anti-fungal agent and a compound of this invention, generally, it is preferred to use the least amount of the anti-fungal compound and of the compound herein that achieves the desired therapeutic or prophylactic effect. This determination is likewise well within the capability of the treating physician.

Packaging The compositions may, if desired, be presented in a pack or dispenser device, such as an FDA approved kit, which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration. The pack or dispenser may also be accompanied by a notice associated with the container in a form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceutical, which notice is reflective of approval by the agency of the form of the compositions or of human or veterinary administration. Such notice, for example, may be of the labeling approved by the U. S. Food and Drug Administration for prescription drugs or of an approved product insert. Compositions comprising a compound of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition. Suitable conditions indicated on the label may include treatment of a fungal infections caused by particular species of fungus and the like.

Biological Activity It is well-know that compounds having at least one asymmetric carbon, and therefore being either R or S at each such carbon as determined by the C-1-P rules, may display different biological activities depending on the absolute stereochemistry at one or more of the asymmetric carbons. In fact, in some instances, only one particular stereochemical configuration may have any biological activity at all. In the compounds of this invention, the carbon to which R2 is bonded (the starred (*) carbon) is asymmetric. The presently preferred absolute stereochemistry at this carbon is S insofar as fungal efflux pump inhibition is concerned. In fact, without being bound to the theory, it is presently believed that only the S configuration has fungal efflux pump inhibitory activity and that activity shown below by racemic mixtures may actually reflect the activity of the S component. No preference is made or suggested regarding the relative or absolute stereochemistry of a compound of this invention when it is used to affect a biological function other than fungal efflux pump inhibition.

In addition to absolute stereochemistry, due to the size of the quinazolinone, piperazine and two benzene rings, the compounds of this invention also exhibit atropisomerism in relation to the single bond between the quinazolinone ring and the carbon to which R2 is bonded, to the single bond between the carbon to which R2 is bonded and the nitrogen of the urea group and to the single bond between the benzene ring to which R4 and R6 are bonded and the urea nitrogen. Again, without being bound to any particular theory, it is believed, similarly to the case of biological preference for a particular absolute and sometimes relative stereochemistry, that one of more of the possible atropisomers of the compounds of this invention may be more active as an efflux pump inhibitor than the others. Thus, the scope of this invention extends to each atropisomer individually as well as to any mixtures thereof.

The activity of test compounds of this invention in combination with an anti- fungal were assessed using a checkerboard assay and the broth microdilution protocol recommended by NCCLS Documents M27-A, Vol. 17, No. 9, June 1997, entitled,"Reference Method for Broth Dilution Anti-fungal Susceptibility Testing of Yeasts, Approved Standard, "and NCCLS Document M38-P, Vol. 18, No. 13, November 1998, entitled,"Reference Method for Broth Dilution Anti-fungal Susceptibility Testing of Conidium-Forming Filamentous Fungi; Proposed Standard. "The test organisms used are Candida albicans YEM15 (over-expressing the CDR1 and CDR2 pumps) and C. glabrata YEM19 (over-expressing the CgCDR1 and CgCDR2 pumps).

Multiple dilutions of a known antifungal and a compound of this invention, are tested alone and in combination at concentrations equal to, above and below the MIC, minimum inhibitory concentration, of the anti-fungal. Unless specifically stated to be otherwise, MIC's are usually reported as the amount of a compound necessary to achieve 80% of the maximum effect possible with that compound. Thus, for example, for an antifungal agent it would be the concentration of the agent that inhibits 80% of the fungal cells contacted. For instance, the MICs of fluconazole against YEM15 and YEM19 are 64 and 128 ug/ml respectively. The compounds of this invention, most of which are shown to have little or no intrinsic anti-fungal activity, are tested at concentration of from about 4 to about 32 pg/ml.

Stock solutions of the test compounds are prepared at a concentration of 64- 128 pglml. Stock solutions are then diluted, according to the needs of a particular assay, in RPMI-1640 with MOPS buffer at 165mM L-glutamine (Angus Buffers & Biochemicals, catalog no. R63165). Stock solutions can be stored at 4°C.

Fluconazole is solubilized according to the instructions of the manufacturers, at a concentration of 10 mg/ml in 100% DMSO. It is then further diluted in RPMI.

The checkerboard assay is normally performed on microtiter plates. Serial dilutions of the anti-fungal are placed in wells horizontally across the plate resulting in each well in any column of wells having the same concentration of the anti-fungal.

The test compound is serially diluted in each column of wells resulting in the rows of wells each containing the same concentration of the test compound. Thus, each well in the array contains a unique combination of anti-fungal and test compound concentrations. Test compounds are examined one per plate.

The assay is performed in RPMI using a final fungal inoculum of 1 to 5 x 103 CFU/ml (from an early-log phase culture). Microtiter plates are incubated for 48 h at 35°C and are read using a microtiter plate reader (Molecular Devices) at 650 nm.

Tables 4 and 5 show potentiation of an anti-fungal agent, as manifested by a reduction in the MIC of the anti-fungal, in the presence, as contrasted to the absence, of an efflux pump inhibitor of this invention.

Table 4 Potentiation of fluconazole vs. C. albicans* Example MPC8<BR> <BR> <BR> <BR> M) C (jug/m)) Number (gglml) ** 1 >32 0. 125 4 >32 2 6 >32 4 7 >32 1 8 >32 2 12 >32 8 13 >32 4 14 >32 2 15 >32 16 18 >32 0.125 20 >332 0.5 21 >32 0.25 23 >32 0.25 24 >32 0.125 25 >32 1 38 >32 0.5 44. >32 0.25 45 >32 1 47 >32 0.25 49 >32 1 53 >32 4 55 >32 4 57 >32 1 62 >32 8 63 >32 8 64 >32 0.125 65 32 0.0625 66 >32 0.25 67 >32 1 68 >32 2 69 >32 0.125 70 >32 0.5 71 >32 2 73 >32 0.5 74 >32 0.25 75 16 0.5 76 >32 0.25 77 >32 0.125 78 >32 0.25 81 >32 0.5 82 >32 8 83 >32 4 85 >32 0.5 86 >32 1 87 >32 0.5 88 >32 1 89 >32 2 90 >32 0.125 91 >32 2 92 >32 4 93 >32 4 95 >32 32 96 >32 32 124 >32 <0. 03 125 >32 <0. 03 126 >32 0.125 130 >32 2 131 >32 4 133 >32 8 137 >32 1 139 >32 2 143 >32 4 145 >32 2 146 >32 0.25 155 >32 8 156 >32 4 157 >32 16 158 >32 16 161 >32 32 162 >32 1 163 >32 2 164 >32 2 165 >32 4 166 >32 1 167 >32 2 173 >32 16 174 >32 0.5 176 >32 32 177 >32 4 191 >32 8 192 >32 1 193 >32 2 196 >32 0.23 198 >32 32 199 >32 16 202 >32 0.06 205 >32 0.25 209 >32 0.25 210 >32 8 211 >32 32 215 >32 1 216 >32 0.5 217 >32 4 218 >32 4 219 >32 8 220 >32 0.25 221 >32 0.125 222 16 0.5 223 16 1 224 >32 1 225 32 1 226 >32 1 227 >32 1 228 >32 4 229 >32 0.125 230 >32 2 232 >32 0.5 234 >32 2 235 >32 0.25 236 >32 0.5 237 >32 4 239 >32 8 241 >32 0.5 242 >32 16 243 >32 16 244 >32 4 245 >32 1 247 >32 0.5 249 >32 2 250 >32 8 251 >32 8 252 >32 0.25 253 >32 16 255 >32 4 256 >32 4 257 >32 0.5 258 >32 8 259 >32 16 261 >32 2 262 >32 2 263 >32 1 264 >32 2 265 >32 2 266 >32 2 267 >32 16 269 >32 0.5 270 >32 1 271 >32 1 272 >32 2 279 >32 1 280 >32 8 281 >32 8 282 >32 8 285 >32 2 286 >32 2 287 >32 0.5 288 >32 0.5 289 >32 1 290 >32 1 291 >32 2 292 >32 2 293 >32 1 294 >32 2 295 >32 1 296 >32 0.25 297 >32 4 298 >32 0.5 299 >32 2 300 >32 0.5 301 >32 2 302 >32 8 303 >32 1 304 >32 2 305 >32 16 306 >32 0.25 307 >32 0.125 308 >32 2 309 >32 0.5 310 >32 2 311 >32 0.5 312 >32 0.5 313 >32 4 315 >32 16 316 >32 16 317 >32 16 318 >32 0.5 319 >32 1 320 >32 1 321 >32 1 322 >32 1 323 >32 4 327 >32 2 328 >32 16 329 >32 16 331 >32 0.5 332 >32 1 333 >32 2 334 >32 0.25 335 >32 1 336 >32 4 337 >32 1 338 >32 0.5 339 >32 0.5 340 >32 2 341 >32 0.5 342 >32 8 343 >32 8 349 >32 4 350 >32 2 351 >32 4 352 >32 2 353 >32 4 354 >32 0.25 355 >32 0.25 356 >32 2 357 >32 8 358 >32 2 359 >32 2 360 >32 1 361 >32 0.5 362 >32 4 363 >32 0.25 364 >32 0.125 365 >32 0.25 366 >32 0.25 367 >32 0.5 368 >32 0.5 369 >32 4 370 >32 0.5 371 >32 0.5 372 >32 2 373 >32 0.5 *Strain YEM15, over-expressing CDR1 and CDR2 efflux pumps **MPC8 = concentration of efflux pump inhibitor necessary to reduce the fluconazole MIC 8-fold Table 5 Potentiation of fluconazole vs. C. glabrata* Compound Number MIC (µg/ml) $MPC8 (µg/ml) 1 >32 2 4 >32 32 6 >32 2 8 >32 8 12 >32 16 13 >32 4 14 >32 2 15 >32 2 18 >32 32 20 >32 1 21 >32 2 22 >32 32 23 >32 2 24 >32 4 25 >32 4 38 >32 0.25 44 >32 2 45 >32 1 53 >32 1 54 >32 4 55 >32 1 57 >32 1 62 >32 32 63 >32 8 64 >32 0.5 65 >32 0.25 66 >32 2 67 >32 2 68 >32 16 69 >32 2 70 >32 4 71 >32 4 73 >32 2 74 >32 1 75 16 0.5 76 >32 0.5 77 >32 0.5 78 >32 0.25 81 >32 1 82 >32 8 83 >32 2 85 >32 0.25 86 >32 2 87 >32 2 88 >32 0.5 89 >32 2 90 >32 4 91 >32 16 93 >32 1 96 >32 8 124 >32 2 125 >32 1 126 >32 4 130 >32 32 131 >32 0.5 132 >32 2 133 >32 16 137 >32 0.5 138 >32 1 139 >32 1 140 >32 2 143 >32 1 145 >32 0.5 146 >32 0.25 155 >32 1 156 >32 2 157 >32 4 158 >32 2 159 >32 2 160 >32 1 161 >32 4 162 >32 0.5 163 >32 2 164 >32 1 165 >32 2 166 >32 0.5 167 >32 2 173 >32 2 174 >32 8 176 >32 16 177 >32 1 183 >32 1 184 >32 32 185 >32 16 186 >32 8 187 >32 4 188 >32 8 189 >32 4 190 >32 0.5 191 >32 2 192 >32 0.5 193 >32 0.25 196 >32 0.5 197 >32 4 198 >32 4 199 >32 2 200 >32 16 202 >32 1 204 >32 16 205 >32 2 206 >32 0.25 208 >32 32 209 >32 1 210 >32 4 211 >32 8 213 >32 16 214 >32 16 215 >32 2 216 >32 0.5 217 >32 4 218 >32 4 219 >32 8 220 >32 0.5 221 >32 0.5 222 16 0.5 223 32 0.25 224 >32 1 225 32 1 226 >32 2 227 >32 1 228 >32 1 229 >32 2 230 >32 4 231 >32 2 232 >32 0.25 233 >32 4 234 >32 0.5 235 >32 0.5 236 >32 0.25 237 >32 0.25 238 >32 2 239 >32 1 240 >32 2 241 >32 0.125 242 >32 4 243 16 2 244 >32 0.5 245 >32 0.25 246 >32 2 247 >32 0.125 248 >32 8 249 >32 0.5 250 >32 1 251 >32 1 252 >32 0.125 253 >32 4 254 >32 4 255 >32 8 256 >32 4 257 >32 0.5 258 >32 8 259 >32 1 260 >32 8 261 >32 2 262 >32 1 263 >32 2 264 >32 1 265 >32 2 266 >32 0.5 267 >32 2 268 >32 4 269 >32 2 270 >32 2 271 >32 2 272 >32 2 273 >32 16 274 >32 4 275 >32 4 276 >32 16 277 >32 16 278 >32 8 279 >32 2 280 >32 2 281 >32 4 282 >32 1 283 >32 4 284 >32 4 285 >32 1 286 >32 1 287 >32 0.25 288 >32 0.25 289 >32 0.125 290 >32 0.25 291 >32 0.5 292 >32 2 293 >32 1 294 >32 2 295 >32 2 296 >32 0.125 297 >32 0. 5 298 >32 0. 5 299 >32 1 300 >32 0.5 301 >32 2 302 >32 2 303 >32 4 304 >32 1 305 >32 4 306 >32 0.0625 307 >32 0.125 308 >32 1 309 >32 0.029 310 >32 0.125 311 >32 1 312 >32 1 313 >32 1 314 >32 2 315 >32 4 316 >32 4 317 >32 0.5 318 >32 1 319 >32 0.5 320 >32 1 321 >32 0.5 322 >32 1 323 >32 0.5 324 >32 2 325 >32 4 326 >32 4 327 >32 2 328 >32 2 329 >32 1 330 >32 2 331 >32 2 332 >32 4 333 >32 0.25 334 >32 0.5 335 >32 0.125 336 >32 0.5 337 >32 2 338 >32 0.5 339 >32 2 340 >32 1 341 >32 2 342 >32 4 343 >32 2 344 >32 8 345 >32 8 346 >32 16 347 >32 2 348 >32 1 349 >32 2 350 >32 1 351 >32 8 352 >32 1 353 >32 8 354 >32 0.5 355 >32 2 356 >32 0.25 357 >32 1 358 >32 0.5 359 >32 0.25 360 >32 1 361 >32 0.125 362 >32 0.5 363 >32 0.029 364 >32 0.0625 365 >32 0.0625 366 >32 0.125 367 >32 0.0625 368 >32 0.25 369 >32 1 370 >32 0.25 371 >32 0.0625 372 >32 0.125 373 >32 0.5 *Strain YEM19, over-expressing CgCDR1 and CgCDR2 efflux pumps CONCLUSION The patents and publications referenced herein are indicative of the level of skill of those skilled in the art to which this invention pertains. All such patents and publications are incorporated by reference to the same extent as if each had been separately incorporated by reference.

While the above description describes particular embodiments and examples illustrating the invention, those skilled in the art will recognize that the invention may be practiced in a variety of alternative ways, for example, by potentiating a variety of other anti-fungal agents that exhibit an efflux pump resistance mechanism. All such variations are within the scope of this invention.

Other embodiments of this invention are contained in the following claims.