Title:
ELECTRON-MICROSCOPIC EXAMINATION METHOD FOR EXAMINING BIOSAMPLE WHILE KEEPING SAID BIOSAMPLE UNCHANGED, AND COMPOSITION FOR EVAPORATION INHIBITION UNDER VACUUM, SCANNING ELECTRON MICROSCOPE, AND TRANSMISSION ELECTRON MICROSCOPE FOR USE IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2013/035866
Kind Code:
A1
Abstract:
A method for electron-microscopic examination is provided with which it is possible to examine a biosample with an electron microscope while keeping the biosample alive and to observe the biosample being moving. Also provided are a composition for evaporation inhibition under vacuum, a scanning electron microscope, and a transmission electron microscope which are for use in the method. This method for sample examination by electron microscopy is characterized by comprising: a step in which a composition for evaporation inhibition that comprises at least one ingredient selected from amphipathic compounds, oils and fats, and ionic liquids is applied to the surface of a sample to form a thin film and thus cover the sample with the thin film; and a step in which an electron-microscopic image of the thin-film-covered sample that has been placed in a vacuum sample chamber is displayed in a display device.
Inventors:
HARIYAMA TAKAHIKO (JP)
TAKAKU YASUHARU (JP)
SUZUKI HIROSHI (JP)
MURANAKA YOSHINORI (JP)
OHTA ISAO (JP)
SHIMOMURA MASATSUGU (JP)
ISHII DAISUKE (JP)
TAKAKU YASUHARU (JP)
SUZUKI HIROSHI (JP)
MURANAKA YOSHINORI (JP)
OHTA ISAO (JP)
SHIMOMURA MASATSUGU (JP)
ISHII DAISUKE (JP)
Application Number:
PCT/JP2012/072982
Publication Date:
March 14, 2013
Filing Date:
September 07, 2012
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
HARIYAMA TAKAHIKO (JP)
TAKAKU YASUHARU (JP)
SUZUKI HIROSHI (JP)
MURANAKA YOSHINORI (JP)
OHTA ISAO (JP)
SHIMOMURA MASATSUGU (JP)
ISHII DAISUKE (JP)
HARIYAMA TAKAHIKO (JP)
TAKAKU YASUHARU (JP)
SUZUKI HIROSHI (JP)
MURANAKA YOSHINORI (JP)
OHTA ISAO (JP)
SHIMOMURA MASATSUGU (JP)
ISHII DAISUKE (JP)
International Classes:
H01J37/20; G01N1/28; G01N23/225; H01J37/18; G01N23/04
Domestic Patent References:
| WO2007083756A1 | 2007-07-26 |
Foreign References:
| JP2010025656A | 2010-02-04 | |||
| JPS612250A | 1986-01-08 | |||
| JP2005026530A | 2005-01-27 | |||
| JPS6188442A | 1986-05-06 | |||
| JP2004319518A | 2004-11-11 | |||
| JPH02147U | 1990-01-05 | |||
| JPH10134751A | 1998-05-22 | |||
| JPS57147857A | 1982-09-11 | |||
| JPH01197626A | 1989-08-09 | |||
| JP2009266741A | 2009-11-12 |
Other References:
See also references of EP 2755225A4
LANGMUIR, vol. 27, 2011, pages 9671 - 9675
MICROSC. RES. TECH., vol. 74, 2011, pages 415 - 420
J. AM. CHEM. SOC., vol. 127, 2005, pages 2398 - 2399
LANGMUIR, vol. 27, 2011, pages 9671 - 9675
MICROSC. RES. TECH., vol. 74, 2011, pages 415 - 420
J. AM. CHEM. SOC., vol. 127, 2005, pages 2398 - 2399
Attorney, Agent or Firm:
NISHIZAWA TOSHIO (JP)
Toshio Nishizawa (JP)
Toshio Nishizawa (JP)
Download PDF:
Claims:
Previous Patent: STEP-UP GEAR FOR WIND-POWERED ELECTRICITY GENERATION
Next Patent: MULTI-NUCLEIC ACID REACTION TOOL, AND DETECTION METHOD USING SAME
Next Patent: MULTI-NUCLEIC ACID REACTION TOOL, AND DETECTION METHOD USING SAME