Title:
MULTI-NUCLEIC ACID REACTION TOOL, AND DETECTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2013/035867
Kind Code:
A1
Abstract:
A multi-nucleic acid reaction tool of an embodiment of the present invention is equipped with a support, a plurality of different primer sets, and probe nucleic acids comprising a complementary sequence of a target sequence. The support is configured to support a liquid-phase reaction field. On at least one surface of the support surface which is in contact with the reaction field when the reaction field has been formed using the liquid phase, the plurality of different primer sets are fixed in a releasable manner to mutually independent primer immobilization regions for each type of primer. Each of the different primer sets is configured to amplify the respective corresponding target sequence. The probe nucleic acids are fixed to the same positions as the primer immobilization regions or to probe immobilization regions in proximity thereto, and hybridized signals are independently detected.
Inventors:
TAKAHASHI MASAYOSHI (JP)
TAKASE MADOKA (JP)
OKADA JUN (JP)
HASHIMOTO KOJI (JP)
NIKAIDO MASARU (JP)
GEMMA NOBUHIRO (JP)
WATANABE KENICHI (JP)
TSUJI KOICHI (JP)
HIROSAWA DAIJI (JP)
YAMAMOTO KEIICHI (JP)
KUWABARA TETSUYA (JP)
SHIMOJO AKIKO (JP)
NAKAMURA AKIYOSHI (JP)
TAKASE MADOKA (JP)
OKADA JUN (JP)
HASHIMOTO KOJI (JP)
NIKAIDO MASARU (JP)
GEMMA NOBUHIRO (JP)
WATANABE KENICHI (JP)
TSUJI KOICHI (JP)
HIROSAWA DAIJI (JP)
YAMAMOTO KEIICHI (JP)
KUWABARA TETSUYA (JP)
SHIMOJO AKIKO (JP)
NAKAMURA AKIYOSHI (JP)
Application Number:
PCT/JP2012/072983
Publication Date:
March 14, 2013
Filing Date:
September 07, 2012
Export Citation:
Assignee:
TOSHIBA KK (JP)
TAKAHASHI MASAYOSHI (JP)
TAKASE MADOKA (JP)
OKADA JUN (JP)
HASHIMOTO KOJI (JP)
NIKAIDO MASARU (JP)
GEMMA NOBUHIRO (JP)
WATANABE KENICHI (JP)
TSUJI KOICHI (JP)
HIROSAWA DAIJI (JP)
YAMAMOTO KEIICHI (JP)
KUWABARA TETSUYA (JP)
SHIMOJO AKIKO (JP)
NAKAMURA AKIYOSHI (JP)
TAKAHASHI MASAYOSHI (JP)
TAKASE MADOKA (JP)
OKADA JUN (JP)
HASHIMOTO KOJI (JP)
NIKAIDO MASARU (JP)
GEMMA NOBUHIRO (JP)
WATANABE KENICHI (JP)
TSUJI KOICHI (JP)
HIROSAWA DAIJI (JP)
YAMAMOTO KEIICHI (JP)
KUWABARA TETSUYA (JP)
SHIMOJO AKIKO (JP)
NAKAMURA AKIYOSHI (JP)
International Classes:
C12M1/00; C12N15/09; C12Q1/68; G01N37/00
Domestic Patent References:
| WO2005054458A1 | 2005-06-16 |
Foreign References:
| JP2003523183A | 2003-08-05 | |||
| JP2005253389A | 2005-09-22 | |||
| JP2011507503A | 2011-03-10 | |||
| JP2006510366A | 2006-03-30 |
Attorney, Agent or Firm:
KURATA, Masatoshi et al. (JP)
Masatoshi Kurata (JP)
Masatoshi Kurata (JP)
Download PDF:
Claims: