Title:
ENGINEERED YEAST FOR FERMENTATION PRODUCTION OF CHONDROITIN SULFATE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/077581
Kind Code:
A1
Abstract:
An engineered yeast for fermentation production of chondroitin sulfate, and use thereof, relating to the technical field of bio-engineering. Using synthetic biology technology and genetic engineering means, and with Pichia pastoris GS115 and Saccharomyces cerevisiae as starting strains, genes related to a chrondroitin sulfate synthesis pathway are expressed heterologously in cells: the genes kfoC and kfoA from Escherichia coli K4, the chondroitin sulfate transferase genes C4ST and C6ST from mice, the UDP-glucose dehydrogenase gene tuaD from Bacillus subtilis, and the ATP sulfurylase gene MET13 from Saccharomyces cerevisiae, to obtain a production strain that synthesizes chrondroitin sulfate A (CSA), chrondroitin sulfate C (CSC), and chrondroitin sulfate E (CSE). For the first time, a microorganism fermentation carbon source is used to synthesize different forms of chrondroitin sulfate.
Inventors:
KANG ZHEN (CN)
JIN XUERONG (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
ZHANG TIANMENG (CN)
JIN XUERONG (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
LI JIANGHUA (CN)
ZHANG TIANMENG (CN)
Application Number:
PCT/CN2019/126014
Publication Date:
April 29, 2021
Filing Date:
December 17, 2019
Export Citation:
Assignee:
BLOOMAGE BIOTECHNOLOGY CORP LTD (CN)
UNIV JIANGNAN (CN)
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N15/81; C12R1/865
Domestic Patent References:
| WO2009149155A1 | 2009-12-10 | |||
| WO1996029424A1 | 1996-09-26 |
Foreign References:
| CN106148265A | 2016-11-23 | |||
| CN106755205A | 2017-05-31 |
Other References:
JIN, XUERONG ET AL.: "Microbial synthesis of glycosaminoglycans with synthetic biology strategies", SCIENTIA SINICA VITAE, vol. 49, no. 5, 14 May 2019 (2019-05-14), pages 553 - 562, XP055803694
ZHOU ZHENGXIONG, DU GUOCHENG, KANG ZHEN: "Production and application of 3-phosphoadenosine-5- phosphosulfate", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 35, no. 7, 25 July 2019 (2019-07-25), CN, pages 1222 - 1233, XP055803698, ISSN: 1000-3061, DOI: 10.13345/j.cjb.180527
ZHOU ZHENGXIONG, LI QING, HUANG HAO, WANG HAO, WANG YANG, DU GUOCHENG, CHEN JIAN, KANG ZHEN: "A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans", BIOTECHNOLOGY AND BIOENGINEERING, WILEY, US, vol. 115, no. 6, 1 June 2018 (2018-06-01), US, pages 1561 - 1570, XP055803702, ISSN: 0006-3592, DOI: 10.1002/bit.26577
CIMINI, D. ; DE ROSA, M. ; VIGGIANI, A. ; RESTAINO, O.F. ; CARLINO, E. ; SCHIRALDI, C.: "Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 150, no. 3, 1 November 2010 (2010-11-01), Amsterdam NL, pages 324 - 331, XP027484000, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2010.09.954
JIN PENG; ZHANG LINPEI; YUAN PANHONG; KANG ZHEN; DU GUOCHENG; CHEN JIAN: "Efficient biosynthesis of polysaccharides chondroitin and heparosan by metabolically engineeredBacillus subtilis", CARBOHYDRATE POLYMERS, APPLIED SCIENCE PUBLISHERS , LTD BARKING, GB, vol. 140, 29 December 2015 (2015-12-29), GB, pages 424 - 432, XP029417158, ISSN: 0144-8617, DOI: 10.1016/j.carbpol.2015.12.065
ZHOU ZHENGXIONG, DU GUOCHENG, KANG ZHEN: "Production and application of 3-phosphoadenosine-5- phosphosulfate", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 35, no. 7, 25 July 2019 (2019-07-25), CN, pages 1222 - 1233, XP055803698, ISSN: 1000-3061, DOI: 10.13345/j.cjb.180527
ZHOU ZHENGXIONG, LI QING, HUANG HAO, WANG HAO, WANG YANG, DU GUOCHENG, CHEN JIAN, KANG ZHEN: "A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans", BIOTECHNOLOGY AND BIOENGINEERING, WILEY, US, vol. 115, no. 6, 1 June 2018 (2018-06-01), US, pages 1561 - 1570, XP055803702, ISSN: 0006-3592, DOI: 10.1002/bit.26577
CIMINI, D. ; DE ROSA, M. ; VIGGIANI, A. ; RESTAINO, O.F. ; CARLINO, E. ; SCHIRALDI, C.: "Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 150, no. 3, 1 November 2010 (2010-11-01), Amsterdam NL, pages 324 - 331, XP027484000, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2010.09.954
JIN PENG; ZHANG LINPEI; YUAN PANHONG; KANG ZHEN; DU GUOCHENG; CHEN JIAN: "Efficient biosynthesis of polysaccharides chondroitin and heparosan by metabolically engineeredBacillus subtilis", CARBOHYDRATE POLYMERS, APPLIED SCIENCE PUBLISHERS , LTD BARKING, GB, vol. 140, 29 December 2015 (2015-12-29), GB, pages 424 - 432, XP029417158, ISSN: 0144-8617, DOI: 10.1016/j.carbpol.2015.12.065
Attorney, Agent or Firm:
ZHONGZI LAW OFFICE (CN)
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