Title:
ENHANCED HETEROLOGOUS PRODUCTION OF LIPOXYGENASES
Document Type and Number:
WIPO Patent Application WO/2014/179207
Kind Code:
A3
Abstract:
The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.
Inventors:
LIU GAOFENG (US)
CHANG MIN-JU (US)
GNATT AVERELL (US)
YE TIAN (US)
CHANG MIN-JU (US)
GNATT AVERELL (US)
YE TIAN (US)
Application Number:
PCT/US2014/035657
Publication Date:
December 30, 2015
Filing Date:
April 28, 2014
Export Citation:
Assignee:
US NEWWIN INC (US)
International Classes:
C12N9/00; A61K38/00; A61K38/46; C12N9/02; C12N9/50; C12P21/04; C12P21/06
Other References:
DEB ET AL.: "Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a 12R-LOX-chaperone complex.", ACTA CRYSTALLOGR SECT F STRUCT BIOL CRYST COMMUN., vol. 67, 2011, pages 903 - 6, XP055245129
ZHANG ET AL.: "Degradation of wood extractives in thermo-mechanical pulp by soybean lipoxygenase.", ENZYME AND MICROBIAL TECHNOLOGY, vol. 40, no. 4, 2007, pages 866 - 873, XP005892509
DANIELSON.: "Addition of Soybean Lipoxygenase to All-Purpose Flour and its Effects on Dough Gluten Strength and Bread Quality.", MASTER OF SCIENCE THESIS., 2007, pages 2, 3, 11, 23 - 26, 29, 39, 62, XP055245132, Retrieved from the Internet [retrieved on 20141009]
VEGA.: "Biocatalysis of Lipoxygenase and Hydroperoxide Lyase.", PHD THESIS, 2008, pages 3, XP055245139, Retrieved from the Internet [retrieved on 20141009]
DATABASE UniProtKB/ Swiss -Prot P0 [o] 3 April 2013 (2013-04-03), Database accession no. LOX1_SOYBN.
INVITROGEN.: "One Shot BL21( DE 3) One Shot BL21( DE 3)pLysS One Shot BL21( DE 3)pLysE Competent Cells.", USER MANUAL., 2 June 2010 (2010-06-02), pages 2, XP055245142, Retrieved from the Internet [retrieved on 20141009]
COLLINS ET AL.: "Use of glycoside hydrolase family 8 xylanases in baking.", JOURNAL OF CEREAL SCIENCE, vol. 43, no. 1, 2006, pages 79 - 84, XP024904947
ZHANG ET AL.: "Degradation of wood extractives in thermo-mechanical pulp by soybean lipoxygenase.", ENZYME AND MICROBIAL TECHNOLOGY, vol. 40, no. 4, 2007, pages 866 - 873, XP005892509
DANIELSON.: "Addition of Soybean Lipoxygenase to All-Purpose Flour and its Effects on Dough Gluten Strength and Bread Quality.", MASTER OF SCIENCE THESIS., 2007, pages 2, 3, 11, 23 - 26, 29, 39, 62, XP055245132, Retrieved from the Internet
VEGA.: "Biocatalysis of Lipoxygenase and Hydroperoxide Lyase.", PHD THESIS, 2008, pages 3, XP055245139, Retrieved from the Internet
DATABASE UniProtKB/ Swiss -Prot P0 [o] 3 April 2013 (2013-04-03), Database accession no. LOX1_SOYBN.
INVITROGEN.: "One Shot BL21( DE 3) One Shot BL21( DE 3)pLysS One Shot BL21( DE 3)pLysE Competent Cells.", USER MANUAL., 2 June 2010 (2010-06-02), pages 2, XP055245142, Retrieved from the Internet
COLLINS ET AL.: "Use of glycoside hydrolase family 8 xylanases in baking.", JOURNAL OF CEREAL SCIENCE, vol. 43, no. 1, 2006, pages 79 - 84, XP024904947
Attorney, Agent or Firm:
REMENICK, James et al. (1025 Thomas Jefferson St. NW,Suite 17, Washington District of Columbia, US)
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