Title:
ENZYMATIC METHOD FOR PREPARING GLUCOSAMINE FROM GLUCOSE, AND ENZYMATIC USE
Document Type and Number:
WIPO Patent Application WO/2022/213477
Kind Code:
A1
Abstract:
Provided is an enzymatic method for preparing glucosamine from glucose. The method comprises: preparing glucosamine by means of taking glucose as a substrate and using same in combination with biological enzymes, preferably a hexokinase, 6-phosphoglucose isomerase, a glucosamine synthase and a glucosamine 6-phosphate dephosphatase. The method has a simple technical route, a sufficient substrate source, mild preparation conditions, low environmental pollution, high reaction specificity, easy downstream separation and purification due to few impurities in the system, and low production cost.
Inventors:
CHEN YANJING (CN)
ZHAN JINMING (CN)
WANG SONGYE (CN)
ZHAN JINMING (CN)
WANG SONGYE (CN)
Application Number:
PCT/CN2021/099567
Publication Date:
October 13, 2022
Filing Date:
June 11, 2021
Export Citation:
Assignee:
JIANGSU AOXIN BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12P19/26; C12P19/24
Foreign References:
| CN106929499A | 2017-07-07 | |||
| CN110387345A | 2019-10-29 | |||
| CN112795605A | 2021-05-14 |
Other References:
LIU DIANLEI, LI PIWU, LI RUIRUI, WANG SHENG, WANG RUIMING: "Research advance of the construction of gene engineering strains for glucosamine", BIOTECHNOLOGY BULLETIN, no. 3, 31 December 2014 (2014-12-31), pages 36 - 41, XP009525425, ISSN: 1002-5464
FANG JIAJU: "Glucokinase is an Isoenzyme of Hexokinase D", CHEMISTRY OF LIFE : COMMUNICATIONS OF THE CHINESE BIOCHEMICAL SOCIETY, SHANGHAI : ZHONGGUO SHENGWU HUAXUEHUI, CN, vol. 12, no. 5, 15 October 1992 (1992-10-15), CN , pages 35 - 35, XP055975283, ISSN: 1000-1336
YOU CHUN: "In Vitro Multi-enzyme System Catalyzed Synthesis of Glucosamine", BAIDU, 29 November 2020 (2020-11-29), pages 270 - 270, XP055975284
DATABASE PROTEIN 3 March 2020 (2020-03-03), ANONYMOUS : "hexokinase type 2 [Drosophila sechellia]", XP055975155, retrieved from NCBI Database accession no. XP_032580865.1
DATABASE PROTEIN 28 May 2019 (2019-05-28), ANONYMOUS : "glucose-6-phosphate isomerase [Deltaproteobacteria bacterium]", XP055975158, retrieved from NCBI Database accession no. TMB57115.1
DATABASE PROTEIN 25 May 2022 (2022-05-25), ANONYMOUS : "RecName: Full=Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; AltName: Full=D-fructose-6-phosphate amidotransferase; AltName: Full=GFAT; AltName: Full=Glucosamine-6-phosphate synthase; AltName: Full=Hexosephosphate aminotransferase; AltName: Fu...", XP055975161, retrieved from NCBI Database accession no. Q56275.3
DATABASE PROTEIN 9 October 2019 (2019-10-09), ANONYMOUS : "MULTISPECIES: kanosamine-6-phosphate phosphatase [Bacillus] ", XP055975163, retrieved from NCBI Database accession no. WP_014479380.1
FANG JIAJU: "Glucokinase is an Isoenzyme of Hexokinase D", CHEMISTRY OF LIFE : COMMUNICATIONS OF THE CHINESE BIOCHEMICAL SOCIETY, SHANGHAI : ZHONGGUO SHENGWU HUAXUEHUI, CN, vol. 12, no. 5, 15 October 1992 (1992-10-15), CN , pages 35 - 35, XP055975283, ISSN: 1000-1336
YOU CHUN: "In Vitro Multi-enzyme System Catalyzed Synthesis of Glucosamine", BAIDU, 29 November 2020 (2020-11-29), pages 270 - 270, XP055975284
DATABASE PROTEIN 3 March 2020 (2020-03-03), ANONYMOUS : "hexokinase type 2 [Drosophila sechellia]", XP055975155, retrieved from NCBI Database accession no. XP_032580865.1
DATABASE PROTEIN 28 May 2019 (2019-05-28), ANONYMOUS : "glucose-6-phosphate isomerase [Deltaproteobacteria bacterium]", XP055975158, retrieved from NCBI Database accession no. TMB57115.1
DATABASE PROTEIN 25 May 2022 (2022-05-25), ANONYMOUS : "RecName: Full=Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; AltName: Full=D-fructose-6-phosphate amidotransferase; AltName: Full=GFAT; AltName: Full=Glucosamine-6-phosphate synthase; AltName: Full=Hexosephosphate aminotransferase; AltName: Fu...", XP055975161, retrieved from NCBI Database accession no. Q56275.3
DATABASE PROTEIN 9 October 2019 (2019-10-09), ANONYMOUS : "MULTISPECIES: kanosamine-6-phosphate phosphatase [Bacillus] ", XP055975163, retrieved from NCBI Database accession no. WP_014479380.1
Attorney, Agent or Firm:
LIANYUNGANG RUNZHI PATENT AGENT OFFICE (CN)
Download PDF: