Title:
ERYTHROCYTE REMOVAL DEVICE, MONONUCLEAR CELL COLLECTOR, CELL CULTURE DEVICE, CELL CULTURE SYSTEM, CELL CULTURE METHOD, AND METHOD FOR COLLECTING MONONUCLEAR CELLS
Document Type and Number:
WIPO Patent Application WO/2020/250927
Kind Code:
A1
Abstract:
Provided is a cell culture method that comprises introducing a factor into cells in a cell culture vessel, and culturing the cells which contain the factor introduced thereinto in the same cell culture vessel as the aforesaid cell culture vessel. Also provided is a method for collecting mononuclear cells, said method comprising: treating blood to give a treated blood from which erythrocytes are at least partially removed; diluting the treated blood; allowing mononuclear cells, which are contained in the treated blood thus diluted, to sediment; removing the supernatant from the diluted treated blood; and then collecting the mononuclear cells.
Inventors:
TANABE KOJI (US)
HIRAIDE RYOJI (JP)
SUTO KENTA (US)
HIRAIDE RYOJI (JP)
SUTO KENTA (US)
Application Number:
PCT/JP2020/022839
Publication Date:
December 17, 2020
Filing Date:
June 10, 2020
Export Citation:
Assignee:
I PEACE INC (US)
TANABE KOJI (US)
TANABE KOJI (US)
International Classes:
C12N5/00
Domestic Patent References:
| WO2018015561A1 | 2018-01-25 |
Foreign References:
| JP2019080575A | 2019-05-30 | |||
| JP2017535282A | 2017-11-30 | |||
| JP2018061519A | 2018-04-19 | |||
| JP4183742B1 | 2008-11-19 | |||
| JP2014114997A | 2014-06-26 |
Other References:
OKIYONEDA TSUKASA ET AL.: "Introduction of the conventional method of DNA transfection by adenovirus vector", FOLIA PHARMACOLOGICA JAPONICA, vol. 119, no. 6, June 2002 (2002-06-01), pages 337 - 344, XP055771677
"Handbook of gene transfer experiments", 1 November 2015, TAKARA BIO INC., JP, article ANONYMOUS: "General protocol of Trans1T-X2", pages: 1 - 12, XP009533495
ANONYMOUS: "Ficoll-Paque(TM) PREMIUM", INSTRUCTIONS 28-4039-56AE CELL PREPARATION MEDIA, 15 October 2005 (2005-10-15), pages 1 - 15, XP055771696
SEKI TOMOHISA ET AL.: "Generating induced pluripotent stem cells from terminally differentiated human peripheral T cells", IGAKU NO AYUMI = JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 239, no. 14, 2011, JP, pages 1320 - 1325, XP009533263, ISSN: 0039-2359
"Handbook of gene transfer experiments", 1 November 2015, TAKARA BIO INC., JP, article ANONYMOUS: "General protocol of Trans1T-X2", pages: 1 - 12, XP009533495
ANONYMOUS: "Ficoll-Paque(TM) PREMIUM", INSTRUCTIONS 28-4039-56AE CELL PREPARATION MEDIA, 15 October 2005 (2005-10-15), pages 1 - 15, XP055771696
SEKI TOMOHISA ET AL.: "Generating induced pluripotent stem cells from terminally differentiated human peripheral T cells", IGAKU NO AYUMI = JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 239, no. 14, 2011, JP, pages 1320 - 1325, XP009533263, ISSN: 0039-2359
Attorney, Agent or Firm:
INABA, Yoshiyuki et al. (JP)
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