Title:
EVALUATION METHOD, COMPLEX, AND KIT
Document Type and Number:
WIPO Patent Application WO/2024/029511
Kind Code:
A1
Abstract:
This evaluation method evaluates whether a protein of interest bonds with a target protein and comprises: a step (a) in which a protein of interest, a target protein, a first specific binding substance for the protein of interest, labeled by a first single-stranded nucleic acid fragment, and a second specific binding substance for the target protein, labeled by a second single-stranded nucleic acid fragment, are brought into contact in a container, as a result, a complex containing the protein of interest, the target protein, and the first and second specific binding substances are formed when the protein of interest bonds with the target protein, and the first single-stranded nucleic acid fragment and the second single-stranded nucleic acid fragment are hybridized to form a double-stranded nucleic acid; and a step (b) in which the formation of a double-stranded nucleic acid is detected. Detection of the formation of a double-stranded nucleic acid indicates bonding of the protein of interest with the target protein.
Inventors:
KUBO YUJI (JP)
MAKINO YOICHI (JP)
MAKINO YOICHI (JP)
Application Number:
PCT/JP2023/028056
Publication Date:
February 08, 2024
Filing Date:
August 01, 2023
Export Citation:
Assignee:
TOPPAN HOLDINGS INC (JP)
International Classes:
C12Q1/6813; C12M1/34; C12Q1/6876; C12N15/09
Domestic Patent References:
| WO2023080137A1 | 2023-05-11 |
Foreign References:
| JP2020122799A | 2020-08-13 | |||
| JP2007020526A | 2007-02-01 |
Other References:
MOHAMMED HISHAM, CARROLL JASON S.: "Approaches for Assessing and Discovering Protein Interactions in Cancer", MOLECULAR CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 11, no. 11, 1 November 2013 (2013-11-01), US , pages 1295 - 1302, XP093135026, ISSN: 1541-7786, DOI: 10.1158/1541-7786.MCR-13-0454
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
Download PDF: