Title:
FENFLURAMINE HAPTEN, AND PREPARATION METHOD THEREFOR AND USE THEREOF, FENFLURAMINE HAPTEN ARTIFICIAL ANTIGEN, AND FENFLURAMINE HAPTEN ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2023/102750
Kind Code:
A1
Abstract:
Provided in the present invention are a fenfluramine hapten, and a preparation method therefor and the use thereof, a fenfluramine artificial antigen, and a fenfluramine antibody. According to the present invention, two haptens, namely hapten FB and hapten FN, are prepared, artificial antigen FB-LF is obtained by means of coupling hapten FB to carrier protein LF, a specific antibody for detecting fenfluramine is further prepared, and artificial antigen FN-OVA is used as a coatingen. The antibody has a good sensitivity and specificity to fenfluramine, with a half-maximal inhibitory concentration of 10.22 ng/mL, a lowest detection limit of 0.63 ng/mL and a quantitative detection range of 1.76-59.13 ng/mL, has no cross reaction to fenfluramine analogs, and can specifically detect fenfluramine. Provided in the present invention is an immunoassay method for fenfluramine, which achieves the purpose of rapidly and accurately detecting fenfluramine.
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Inventors:
LEI HONGTAO (CN)
PAN KANGLIANG (CN)
QUAN QIQI (CN)
FANG YALIN (CN)
GUAN TIAN (CN)
WANG JIN (CN)
SHEN XING (CN)
LI XIANGMEI (CN)
XU ZHENLIN (CN)
PAN KANGLIANG (CN)
QUAN QIQI (CN)
FANG YALIN (CN)
GUAN TIAN (CN)
WANG JIN (CN)
SHEN XING (CN)
LI XIANGMEI (CN)
XU ZHENLIN (CN)
Application Number:
PCT/CN2021/136186
Publication Date:
June 15, 2023
Filing Date:
December 07, 2021
Export Citation:
Assignee:
UNIV SOUTH CHINA AGRICULT (CN)
International Classes:
C07C229/14; C07C227/08; C07C227/18; C07K14/765; C07K14/77; C07K14/79; C07K14/795; C07K16/06; C07K16/44; G01N33/53; G01N33/543; G01N33/577
Foreign References:
| CN112321443A | 2021-02-05 |
Other References:
DATABASE REGISTRY 24 November 2016 (2016-11-24), ANONYMOUS : "Butanoic acid, 4-[[1-met hyl-2-[3-(trifluorome thyl)phenyl]ethyl]a mino]", XP093070811, retrieved from STN Database accession no. 2036831-43-1
DATABASE REGISTRY 18 March 2014 (2014-03-18), ANONYMOUS : "Hexanoic acid, 6-[[2-[3-(t rifluoromethyl)phen yl]acetyl]amino]", XP093070817, retrieved from STN Database accession no. 1569551-14-9
"Veterinary Drug Residue Analysis", 28 February 2002, SHANGHAI SCIENCE AND TECHNOLOGY PRESS, CN, ISBN: 7-5323-6282-5, article LI, JUNSUO ET AL.: "Passage; Chapter 4 Immunoassays", pages: 156 - 165, XP009546825
"Immunology and Pathogen Detection Technology and Basic and Innovative Experiments", 31 July 2013, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY PRESS, CN, ISBN: 978-7-5609-8920-4, article ZENG, QINGREN ET AL.: "Passage; Immunology and Pathogen Detection Technology and Basic and Innovative Experiments", pages: 53 - 53, XP009546826
DATABASE REGISTRY 18 March 2014 (2014-03-18), ANONYMOUS : "Hexanoic acid, 6-[[2-[3-(t rifluoromethyl)phen yl]acetyl]amino]", XP093070817, retrieved from STN Database accession no. 1569551-14-9
"Veterinary Drug Residue Analysis", 28 February 2002, SHANGHAI SCIENCE AND TECHNOLOGY PRESS, CN, ISBN: 7-5323-6282-5, article LI, JUNSUO ET AL.: "Passage; Chapter 4 Immunoassays", pages: 156 - 165, XP009546825
"Immunology and Pathogen Detection Technology and Basic and Innovative Experiments", 31 July 2013, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY PRESS, CN, ISBN: 978-7-5609-8920-4, article ZENG, QINGREN ET AL.: "Passage; Immunology and Pathogen Detection Technology and Basic and Innovative Experiments", pages: 53 - 53, XP009546826
Attorney, Agent or Firm:
YOGO PATENT AND TRADEMARK AGENCY LIMITED COMPANY (CN)
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