Title:
GENETICALLY ENGINEERED BACTERIUM FOR SYNTHESIZING P-COUMARIC ACID AND DERIVATIVE THEREOF, METHOD FOR CONSTRUCTING SAME AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/164985
Kind Code:
A1
Abstract:
A genetically engineered bacterium for synthesizing p-coumaric acid and derivatives thereof, a method for constructing same and use thereof. The genetically engineered bacterium for synthesizing p-coumaric acid comprises a chassis strain into which a p-coumaric acid synthesis gene has been introduced, and the chassis strain has also been subjected to any one of or a combination of at least two of the following genetic modifications: (1) deleting aro8 and aro9; (2) deleting tdh1, tdh2, tdh3, and overexpressing GAPB; (3) deleting idh1 or idh2, and simultaneously overexpressing YHM2 and IDP2; (4) deleting pgi1, pfk1, or pfk2, and simultaneously overexpressing ZWF1, GND1, RPE1, RKI1, TKL1, and TAL1; (5) overexpressing POS5; or (6) deleting gdh2. Efficient synthesis of p-coumaric acid and derivatives thereof is realized.
Inventors:
YU TAO (CN)
LUO WEI (CN)
GUO SHUYUAN (CN)
LUO WEI (CN)
GUO SHUYUAN (CN)
Application Number:
PCT/CN2022/083391
Publication Date:
September 07, 2023
Filing Date:
March 28, 2022
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12N1/19; C12N15/81; C12P7/22; C12R1/865
Foreign References:
| EP3656857A1 | 2020-05-27 | |||
| US20190002892A1 | 2019-01-03 | |||
| CN110628657A | 2019-12-31 | |||
| CN108315290A | 2018-07-24 | |||
| CN109609421A | 2019-04-12 | |||
| CN108949652A | 2018-12-07 | |||
| CN106032525A | 2016-10-19 |
Other References:
ZHANG, SIQI ET AL.: "Construction and Optimization of P-coumaric Acid-producing Saccharomyces Cerevisiae", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 36, no. 09, 25 September 2020 (2020-09-25), pages 1838 - 1848, XP009548830
LIU QUANLI, YU TAO, LI XIAOWEI, CHEN YU, CAMPBELL KATE, NIELSEN JENS, CHEN YUN: "Rewiring carbon metabolism in yeast for high level production of aromatic chemicals", NATURE COMMUNICATIONS, vol. 10, no. 1, XP093026414, DOI: 10.1038/s41467-019-12961-5
ZHANG, WEI; LIU, DUO; LI, BING-ZHI; YUAN, YING-JIN: "Construction and Optimization of P-coumaric Acid Producing Saccharomyces Cerevisiae", ZHONGGUO SHENGWU GONGCHENG ZAZHI - CHINA BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WENXIAN QINGBAO ZHONGXIN,CHINESE ACADEMY OF SCIENCES, DOCUMENTATION AND INFORMATION CENTER, CN, vol. 37, no. 9, 15 September 2017 (2017-09-15), CN, pages 89 - 97, XP009548831, ISSN: 1671-8135, DOI: 10.13523/j.cb.20170912
LIU QUANLI, YU TAO, LI XIAOWEI, CHEN YU, CAMPBELL KATE, NIELSEN JENS, CHEN YUN: "Rewiring carbon metabolism in yeast for high level production of aromatic chemicals", NATURE COMMUNICATIONS, vol. 10, no. 1, XP093026414, DOI: 10.1038/s41467-019-12961-5
ZHANG, WEI; LIU, DUO; LI, BING-ZHI; YUAN, YING-JIN: "Construction and Optimization of P-coumaric Acid Producing Saccharomyces Cerevisiae", ZHONGGUO SHENGWU GONGCHENG ZAZHI - CHINA BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WENXIAN QINGBAO ZHONGXIN,CHINESE ACADEMY OF SCIENCES, DOCUMENTATION AND INFORMATION CENTER, CN, vol. 37, no. 9, 15 September 2017 (2017-09-15), CN, pages 89 - 97, XP009548831, ISSN: 1671-8135, DOI: 10.13523/j.cb.20170912
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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