Title:
GENOTYPE IDENTIFICATION OF MULTI-PARENT CROP ON BASIS OF HIGH-THROUGHPUT WHOLE GENOME SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2022/160700
Kind Code:
A1
Abstract:
Genotype identification of a multi-parent crop on the basis of high-throughput whole genome sequencing. Specifically, the method comprises: (a) for n parents and the progeny thereof, providing sequencing data Df of a progeny crop to be identified and sequencing data Dp of a parent crop corresponding to the progeny crop, with n being a positive integer of ≥ 3; (b) determining SNP site information of the parent and the progeny on the basis of the sequencing data Df and the sequencing data Dp; (c) determining the genotype of the progeny on the basis of the SNP site information, thereby obtaining an evaluation result of each SNP of the progeny and distribution information of the recombinant breaking point on each chromosome of the whole genome of the progeny; and (d) constructing and/or drawing a genotype map of the progeny, thereby obtaining a genotype identification result of a multi-parent crop. The method can be used for identifying the genotype of the multi-parent crop with high throughput, rapidness and accuracy.
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Inventors:
HAN BIN (CN)
ZHU ZHOU (CN)
WANG AHONG (CN)
ZHU ZHOU (CN)
WANG AHONG (CN)
Application Number:
PCT/CN2021/115146
Publication Date:
August 04, 2022
Filing Date:
August 27, 2021
Export Citation:
Assignee:
CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)
International Classes:
G16B30/00; G16B20/00
Foreign References:
| CN111508560A | 2020-08-07 | |||
| CN104328507A | 2015-02-04 |
Other References:
X. HUANG, Q. FENG, Q. QIAN, Q. ZHAO, L. WANG, A. WANG, J. GUAN, D. FAN, Q. WENG, T. HUANG, G. DONG, T. SANG, B. HAN: "High-throughput genotyping by whole-genome resequencing", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 19, no. 6, 1 June 2009 (2009-06-01), US , pages 1068 - 1076, XP055577519, ISSN: 1088-9051, DOI: 10.1101/gr.089516.108
LUO LONGHAI, YUE GUIDONG, GAO QIANG, WANG JUNYI, XU JIAOHUI, YIN YE: "The Application of High-throughput Sequencing Technology in Plant and Animal Research", SCIENCE CHINA: CHINESE BULLETIN OF LIFE SCIENCE = SCIENTIA SINICA VITAE, vol. 42, no. 2, 1 February 2012 (2012-02-01), pages 107 - 124, XP055954237, ISSN: 1674-7232, DOI: 10.1360/052011-634
XUE YONGBIAO, HAN BIN, CHONG KANG, WANG TAI, HE ZUHUA, FU XIANGDONG, CHU CHENGCAI, CHENG ZHUKUAN, XU YUNYUAN, LI MING: "Achievements and Prospect of Designer Breeding by Molecular Modules in Rice ", BULLETIN OF CHINESE ACADEMY OF SCIENCES, vol. 33, no. 9, 1 September 2018 (2018-09-01), pages 1 - 10, XP055954240, ISSN: 1000-3045, DOI: 10.16418/j.issn.1000-3045.2018.09.002
QING-QING HOU, LI-ZHEN SI, XUE-HUI HUANG, BIN HAN: "Progress on genome-wide association study of important agronomic traits in rice", CHINESE BULLETIN OF LIFE SCIENCES, vol. 28, no. 10, 1 October 2016 (2016-10-01), pages 1 - 8, XP055954244, ISSN: 1004-0374, DOI: 10.13376/j.cbls/2016162
LU WANG; AHONG WANG; XUEHUI HUANG; QIANG ZHAO; GUOJUN DONG; QIAN QIAN; TAO SANG; BIN HAN: "Mapping 49 quantitative trait loci at high resolution through sequencing-based genotyping of rice recombinant inbred lines", THEORETICAL AND APPLIED GENETICS ; INTERNATIONAL JOURNAL OF PLANT BREEDING RESEARCH, SPRINGER, BERLIN, DE, vol. 122, no. 2, 28 September 2010 (2010-09-28), Berlin, DE , pages 327 - 340, XP019873367, ISSN: 1432-2242, DOI: 10.1007/s00122-010-1449-8
LUO LONGHAI, YUE GUIDONG, GAO QIANG, WANG JUNYI, XU JIAOHUI, YIN YE: "The Application of High-throughput Sequencing Technology in Plant and Animal Research", SCIENCE CHINA: CHINESE BULLETIN OF LIFE SCIENCE = SCIENTIA SINICA VITAE, vol. 42, no. 2, 1 February 2012 (2012-02-01), pages 107 - 124, XP055954237, ISSN: 1674-7232, DOI: 10.1360/052011-634
XUE YONGBIAO, HAN BIN, CHONG KANG, WANG TAI, HE ZUHUA, FU XIANGDONG, CHU CHENGCAI, CHENG ZHUKUAN, XU YUNYUAN, LI MING: "Achievements and Prospect of Designer Breeding by Molecular Modules in Rice ", BULLETIN OF CHINESE ACADEMY OF SCIENCES, vol. 33, no. 9, 1 September 2018 (2018-09-01), pages 1 - 10, XP055954240, ISSN: 1000-3045, DOI: 10.16418/j.issn.1000-3045.2018.09.002
QING-QING HOU, LI-ZHEN SI, XUE-HUI HUANG, BIN HAN: "Progress on genome-wide association study of important agronomic traits in rice", CHINESE BULLETIN OF LIFE SCIENCES, vol. 28, no. 10, 1 October 2016 (2016-10-01), pages 1 - 8, XP055954244, ISSN: 1004-0374, DOI: 10.13376/j.cbls/2016162
LU WANG; AHONG WANG; XUEHUI HUANG; QIANG ZHAO; GUOJUN DONG; QIAN QIAN; TAO SANG; BIN HAN: "Mapping 49 quantitative trait loci at high resolution through sequencing-based genotyping of rice recombinant inbred lines", THEORETICAL AND APPLIED GENETICS ; INTERNATIONAL JOURNAL OF PLANT BREEDING RESEARCH, SPRINGER, BERLIN, DE, vol. 122, no. 2, 28 September 2010 (2010-09-28), Berlin, DE , pages 327 - 340, XP019873367, ISSN: 1432-2242, DOI: 10.1007/s00122-010-1449-8
Attorney, Agent or Firm:
XU&PARTNERS, LLC. (CN)
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