Title:
GLUFOSINATE DEHYDROGENASE MUTANT AND APPLICATION THEREOF IN SYNTHESIZING L-GLUFOSINATE
Document Type and Number:
WIPO Patent Application WO/2020/133990
Kind Code:
A1
Abstract:
The present invention discloses a glufosinate dehydrogenase mutant and an application thereof in synthesizing L-glufosinate, the method using 2-carbonyl-4-(hydroxy-methyl-phosphoryl) butyric acid or a salt thereof as a substrate; in the presence of an inorganic amino donor and a reduced coenzyme, using glufosinate dehydrogenase or a cell containing glufosinate dehydrogenase as a biocatalyst, performing a reductive amination reaction to obtain L-glufosinate. The method of the present invention exhibits a high conversion rate and yield, the product is easily separated and purified, and has relatively high chiral purity; relative to other catalytic processes, the present process is simple and has a raw material conversion rate of up to 100%.
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Inventors:
XUE YAPING (CN)
CHENG FENG (CN)
LI QINGHUA (CN)
ZHENG YUGUO (CN)
XU JIANMIAO (CN)
CHENG FENG (CN)
LI QINGHUA (CN)
ZHENG YUGUO (CN)
XU JIANMIAO (CN)
Application Number:
PCT/CN2019/093462
Publication Date:
July 02, 2020
Filing Date:
June 28, 2019
Export Citation:
Assignee:
UNIV ZHEJIANG TECHNOLOGY (CN)
International Classes:
C12N1/21; C12N9/06; C12N15/53; C12P13/04; C12R1/19
Foreign References:
| CN109609475A | 2019-04-12 | |||
| CN108588045A | 2018-09-28 | |||
| CN107502647A | 2017-12-22 |
Other References:
DATABASE NCBI 6 August 2017 (2017-08-06), ANONYMOUS: "NADP-specific glutamate dehydrogenase[Pseudomonas moorei", XP055722044, retrieved from Protein Database accession no. WP090325311. 1
Attorney, Agent or Firm:
TIANZHENG PATENT ATTORNEYS (CN)
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