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Title:
AMINO ACID DEHYDROGENASE MUTANT AND USE THEREOF IN SYNTHESIS OF L-GLUFOSINATE
Document Type and Number:
WIPO Patent Application WO/2020/133989
Kind Code:
A1
Abstract:
Disclosed are an amino acid dehydrogenase mutant and the use thereof in the synthesis of L-glufosinate. The amino acid dehydrogenase mutant is obtained by performing a single mutation or multiple mutations on amino acids at positions 95, 108, 172 and 303 shown in SEQ ID No.2. The specific enzyme activity of the prepared amino acid dehydrogenase mutant DyGDH-F95I-A108T-R172P -R303H is 33 times higher than that of the maternal aldo-keto reductase, and the maximum feeding amount of a substrate 2-carbonyl-4-(hydroxyl methyl phosphinyl)-butyric acid reaches 500 mM. The amino acid dehydrogenase mutant has better industrial application prospects. By using the amino acid dehydrogenase mutant to produce L-glufosinate, the reaction time is significantly shortened, from generally taking 20 hours to now only taking 120 minutes, showing that the amino acid dehydrogenase mutant has a good application prospect.

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Inventors:
XUE YAPING (CN)
CHENG FENG (CN)
LI HENG (CN)
ZHENG YUGUO (CN)
XU JIANMIAO (CN)
Application Number:
PCT/CN2019/093453
Publication Date:
July 02, 2020
Filing Date:
June 28, 2019
Export Citation:
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Assignee:
UNIV ZHEJIANG TECHNOLOGY (CN)
International Classes:
C12N1/21; C12N9/06; C12N15/54; C12P13/04
Domestic Patent References:
WO2002036742A22002-05-10
WO2006015885A12006-02-16
WO2016001362A12016-01-07
Foreign References:
CN109609474A2019-04-12
CN108588045A2018-09-28
CN109022381A2018-12-18
CN108795893A2018-11-13
CN106978453A2017-07-25
Other References:
DATABASE Protein 4 August 2017 (2017-08-04), "NADP-specific glutamate dehydrogenase[Pseudomonas monteilii", XP055722854, retrieved from NCBI Database accession no. WP_060477601.1
YIN, X. ET AL: "Efficient Reductive Amination Process for Enantioselective Synthesis of L-Phosphinothricin Applying Engineered Glutamate Dehydrogenase", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 102, no. 10, 16 March 2019 (2019-03-16), pages 4425 - 4433, XP036493130
Attorney, Agent or Firm:
TIANZHENG PATENT ATTORNEYS (CN)
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