Title:
GLYCORANDOMIZATION AND THE PRODUCTION OF NOVEL ERYTHRONOLIDE AND COUMARIN ANALOGS
Document Type and Number:
WIPO Patent Application WO2002079150
Kind Code:
A3
Abstract:
The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or one which is putatively associated with or involved in production of natural secondary metabolites. The glycosyltransferase may show significant flexibility with respect to its NDP-sugar donors and/or its aglycons. NDP-sugar donors may be commercially available, or may be produced by utilizing mutant or wild type nucleotidyltransferases significant flexibility with respect to their substrates.
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Inventors:
THORSON JON S (US)
Application Number:
PCT/US2002/009841
Publication Date:
December 08, 2005
Filing Date:
April 01, 2002
Export Citation:
Assignee:
SLOAN KETTERING INST CANCER (US)
THORSON JON S (US)
THORSON JON S (US)
International Classes:
C07H1/00; C07H15/203; C07H17/08; C12N15/54; (IPC1-7): C12P19/18; C12P19/30
Domestic Patent References:
| WO1999031224A2 | 1999-06-24 |
Other References:
THORSON J.S. ET AL: "Nature's Carbohydrate Chemist: The Enzymatic Glycosylation of Bioactive Bacterial Metabolites.", CURRENT ORGANIC CHEMISTRY., vol. 5, no. 2, 2001, pages 139 - 167, XP002992232
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