Title:
HIGH AFFINITY MONOCLONAL ANTIBODY FOR RECOGNIZING THE ESTROGEN RECEPTOR (ER) AND METHOD FOR CREATING THE ANTIBODY
Document Type and Number:
WIPO Patent Application WO2004024747
Kind Code:
A3
Abstract:
High affinity monoclonal antibodies for recognizing estrogen receptor (clone SP1) with immunohistochemistry and methods for creating such an antibody are disclosed. The lagomorph derived ER antibody provides a significant advantage over the currently available mouse ER antibodies in that there is no need for target retrieval when performing immunohistochemistry. Furthermore, the very low background when the lagomorph derived ER antibody is used in immunohistochemistry is also impressive. The immunohistochemistry comparative study with about fifty clinical specimens showed that the new ER (clone SP1) antibody and favorable results when compared to mouse monoclonal ER antibodies (clone 1D5). The lagomorph derived ER antibody may prove of great value in the assessment of ER status in human breast cancer. Humanized versions of the ER antibody may also provide therapeutic benefits.
Inventors:
XIA HAIYING
HUANG ZHIDA
HUANG ZHIDA
Application Number:
PCT/US2003/028771
Publication Date:
August 26, 2004
Filing Date:
September 11, 2003
Export Citation:
Assignee:
SPRING BIOSCIENCE (US)
International Classes:
C07K16/00; C07K16/28; C12P21/08; (IPC1-7): C07K16/28; C12P21/08; G01N33/531; G01N33/543; G01N33/566; G01N33/574
Foreign References:
| US5675063A | 1997-10-07 |
Other References:
SCHUH ET AL: "Polyclonal Antibodies from Rabbits and Chickens Against the Estrogen Receptor and Related Peptides", RECEPTOR, vol. 2, 1992, pages 93 - 107, XP002978264
BROMLEY ET AL: "Preservation of Estrogen Receptor in Paraffin Sections", THE JOURNAL OF HISTOTECHNOLOGY, vol. 17, 1994, pages 115 - 118, XP002978407
ALI ET AL: "Production and Characterization of Monoclonal Antibodies Recognising Defined Regions of the Human Oestrogen Receptor", HYBRIDOMA, vol. 12, no. 4, 1993, pages 391 - 405, XP002978408
HARLOW ET AL: "Antibodies. A Laboratory Manual", 1988, COLD SPRING HARBOR: COLD SPRING HARBOR LABORATORY, pages: 72 - 77, XP002914828
OWENS ET AL: "The Genetic Engineering of Monoclonal Antibodies", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 168, 1994, pages 149 - 165, XP002914829
BROMLEY ET AL: "Preservation of Estrogen Receptor in Paraffin Sections", THE JOURNAL OF HISTOTECHNOLOGY, vol. 17, 1994, pages 115 - 118, XP002978407
ALI ET AL: "Production and Characterization of Monoclonal Antibodies Recognising Defined Regions of the Human Oestrogen Receptor", HYBRIDOMA, vol. 12, no. 4, 1993, pages 391 - 405, XP002978408
HARLOW ET AL: "Antibodies. A Laboratory Manual", 1988, COLD SPRING HARBOR: COLD SPRING HARBOR LABORATORY, pages: 72 - 77, XP002914828
OWENS ET AL: "The Genetic Engineering of Monoclonal Antibodies", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 168, 1994, pages 149 - 165, XP002914829
Download PDF: