Title:
HIGH AFFINITY MONOCLONAL ANTIBODY FOR RECOGNIZING THE PROGESTERONE RECEPTOR (PR) AND METHOD FOR CREATING THE ANTIBODY
Document Type and Number:
WIPO Patent Application WO2004024748
Kind Code:
A3
Abstract:
A lagomorph derived monoclonal antibody for recognizing the progesterone receptor (PR) (clone SP2) with immunohistochemistry and methods for creating such an antibody are disclosed. No need of target retrieval in immunohistochemistry is their prominent advantage compared to currently available PR antibody. Furthermore, the very low background when the lagomorph derived monoclonal antibody is used in immunohistochemistry is also impressive. The immunohistochemistry comparative study with about fifty clinical specimens showed that the new PR antibody (clone SP2) antibody had favorable results when compared to mouse monoclonal antibody PR (clone 1A6), the best one in the current market. The PR antibody may prove of great value in the assessment of PR status in human breast cancer. Humanized versions of the PR antibody may provide therapeutic benefits.
Inventors:
XIA HAIYING
HUANG ZHIDA
HUANG ZHIDA
Application Number:
PCT/US2003/028772
Publication Date:
October 28, 2004
Filing Date:
September 11, 2003
Export Citation:
Assignee:
SPRING BIOSCIENCE (US)
International Classes:
C07K16/28; C12P21/08; (IPC1-7): C07K16/28; C12P21/08; G01N33/531; G01N33/543; G01N33/566; G01N33/574
Foreign References:
| US4742000A | 1988-05-03 | |||
| US5283190A | 1994-02-01 | |||
| US5675063A | 1997-10-07 |
Other References:
MISRAHI: "Complete Amino Acid Sequence of the Human Progesterone Receptor Deduced from Cloned cDNA", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 143, no. 2, 13 March 1987 (1987-03-13), pages 740 - 748, XP002112420
HARLOW ET AL.: "Antibodies. A Laboratory Manual", 1988, COLD SPRING HARBOR, pages: 72 - 77, XP002920436
OWENS: "The Genetic Engineering of Monoclonal Antibodies", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 168, 1994, pages 149 - 165, XP002914829
MOTE ET AL.: "Detection of Progesterone Receptor Forms A and B by Immunohistochemical Analysis", JOURNAL OF CLINICAL PATHOLOGY, vol. 54, no. 8, August 2001 (2001-08-01), pages 624 - 630, XP002975058
HARLOW ET AL.: "Antibodies. A Laboratory Manual", 1988, COLD SPRING HARBOR, pages: 72 - 77, XP002920436
OWENS: "The Genetic Engineering of Monoclonal Antibodies", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 168, 1994, pages 149 - 165, XP002914829
MOTE ET AL.: "Detection of Progesterone Receptor Forms A and B by Immunohistochemical Analysis", JOURNAL OF CLINICAL PATHOLOGY, vol. 54, no. 8, August 2001 (2001-08-01), pages 624 - 630, XP002975058
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