Title:
HIGH-THROUGHPUT SEQUENCING METHOD FOR METHYLATED CPG ISLAND IN TRACE DNA
Document Type and Number:
WIPO Patent Application WO/2017/147946
Kind Code:
A1
Abstract:
Disclosed is a high-throughput sequencing method for a methylated CpG island in trace DNA, comprising the steps of: 1) treating a DNA sample with sulphite; 2) adding the treated sample obtained in step 1) to a PCR system containing a primer A for linear amplification; 3) adding an exonuclease having single-stranded DNA cleaving activity to the PCR product in step 2) and inactivating the exonuclease after the reaction; 4) adding the product in step 3) to a PCR system containing a primer B for linear amplification; 5) adding the product in step 4) to a PCR system containing the corresponding linker primer C and linker primer D for amplification; and 6) purifying the PCR product in step 5) to obtain a DNA library with a specific length and carrying out the sequencing.
Inventors:
LU XINGYU (CN)
SONG YANQUN (CN)
SONG YANQUN (CN)
Application Number:
PCT/CN2016/075864
Publication Date:
September 08, 2017
Filing Date:
March 08, 2016
Export Citation:
Assignee:
SHANGHAI EPICAN GENETECH CO LTD (CN)
International Classes:
C12Q1/68
Foreign References:
| CN104250663A | 2014-12-31 | |||
| CN104673931A | 2015-06-03 | |||
| CN105256018A | 2016-01-20 |
Attorney, Agent or Firm:
SHANGHAI CPTO INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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