Title:
SEQUENCING METHOD FOR GENETIC MAPPING OF DNA 5-METHYLCYTOSINE AND 5-HYDROXYMETHYLCYTOSINE
Document Type and Number:
WIPO Patent Application WO/2017/147945
Kind Code:
A1
Abstract:
Provided is a sequencing method for the genetic mapping of DNA 5-methylcytosine and 5-hydroxymethylcytosine. The method employs next generation sequencing and library construction technology for trace free DNA and a high-efficiency chemical bioorthogonal reaction, which greatly enhances the selectivity and efficiency of a solid-phase surface binding to a DNA modified base.
Inventors:
LU XINGYU (CN)
SONG YANQUN (CN)
SONG YANQUN (CN)
Application Number:
PCT/CN2016/075863
Publication Date:
September 08, 2017
Filing Date:
March 08, 2016
Export Citation:
Assignee:
SHANGHAI EPICAN GENETECH CO LTD (CN)
International Classes:
C12Q1/68; C12N15/10; C40B50/06
Domestic Patent References:
| WO2005121361A2 | 2005-12-22 |
Foreign References:
| CN103131754A | 2013-06-05 | |||
| CN1463292A | 2003-12-24 | |||
| CN104480214A | 2015-04-01 |
Attorney, Agent or Firm:
SHANGHAI CPTO INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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