Title:
HPLC METHOD FOR DETECTING BIOTIN INTERMEDIATE DIAMINO SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2021/128665
Kind Code:
A1
Abstract:
Disclosed is an HPLC method for detecting a biotin intermediate diamino substance. The method comprises the following steps: dissolving a cyclization reaction solution in a biotin synthesis process by means of using a mobile phase, and then, carrying out detecting under the following chromatographic conditions: the chromatographic columns being C18-WCX mixed columns, the detection wavelength being 195-220 nm, the test time being 16-20 min, and the mobile phase being an acetonitrile-phosphate buffer solution. According to the detection method, the appearance time of a substance to be detected, a diamino substance, can be stabilized within 10-12 min, so that the problem of peak superposition of the diamino substance and a solvent peak is solved, and testing is more accurate and reliable.
Inventors:
LV GUOFENG (CN)
WANG CHANGZE (CN)
SHAN GUOHONG (CN)
HANG JIAO (CN)
WANG ZHENZHEN (CN)
LI XIAOJUN (CN)
DUAN WENJIE (CN)
WEI NIANXIANG (CN)
WANG CHANGZE (CN)
SHAN GUOHONG (CN)
HANG JIAO (CN)
WANG ZHENZHEN (CN)
LI XIAOJUN (CN)
DUAN WENJIE (CN)
WEI NIANXIANG (CN)
Application Number:
PCT/CN2020/085714
Publication Date:
July 01, 2021
Filing Date:
April 20, 2020
Export Citation:
Assignee:
SHANGYU NHU BIO CHEM CO LTD (CN)
ZHEJIANG NHU PHARMACEUTICAL CO LTD (CN)
ZHEJIANG NHU PHARMACEUTICAL CO LTD (CN)
International Classes:
G01N30/02; G01N30/86
Foreign References:
| CN110887913A | 2020-03-17 | |||
| CN101504393A | 2009-08-12 | |||
| CN105418633A | 2016-03-23 | |||
| JP2015118029A | 2015-06-25 |
Other References:
CHASTAIN, J.L. ; BOWERS-KOMRO, D.M. ; MCCORMICK, D.B.: "High-performance liquid chromatography of biotin and analogues", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 330, 1 January 1985 (1985-01-01), AMSTERDAM, NL, pages 153 - 158, XP026557023, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(01)81971-3
XUEFAN CHEN, CHUCHU HU, PANPAN ZHAO, GUOGANG ZHENG: "Determination of Related Substances in Biotin by HPLC", CHINESE JOURNAL OF PHARMACEUTICALS, SHANGHAI YIYAO GONGYE YANJIUYUAN,SHANGHAI INSTITUTE OF PHARMACEUTICAL INDUSTRY, CN, vol. 45, no. 11, 1 January 2014 (2014-01-01), CN, pages 1066 - 1069, XP055827309, ISSN: 1001-8255, DOI: 10.16522/j.cnki.cjph.2014.11.004
XUEFAN CHEN, CHUCHU HU, PANPAN ZHAO, GUOGANG ZHENG: "Determination of Related Substances in Biotin by HPLC", CHINESE JOURNAL OF PHARMACEUTICALS, SHANGHAI YIYAO GONGYE YANJIUYUAN,SHANGHAI INSTITUTE OF PHARMACEUTICAL INDUSTRY, CN, vol. 45, no. 11, 1 January 2014 (2014-01-01), CN, pages 1066 - 1069, XP055827309, ISSN: 1001-8255, DOI: 10.16522/j.cnki.cjph.2014.11.004
Attorney, Agent or Firm:
JJS INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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