Title:
IMMOBILIZATION METHOD OF LIQUID LIPASE AND PREPARATION METHOD OF SUCROSE-6-ACETATE
Document Type and Number:
WIPO Patent Application WO/2022/151024
Kind Code:
A1
Abstract:
An immobilization method of a liquid lipase. The immobilization method comprises the following steps performed in sequence: a dissolving step: adding alginate into deionized water to completely dissolve, and adding a liquid lipase at a preset temperature to obtain a mixed solution; an immobilization step: mixing the mixed solution with a metal cross-linking agent solution, and maintaining for a preset time period to obtain a spherical gel, so that the liquid lipase is immobilized; and a chelating step: after the spherical gel is washed, adding a buffer solution and an organic complexing agent solution to perform a chelating reaction, and then washing with a buffer solution to obtain the immobilized liquid lipase. The obtained gel has extremely high physical strength, can be more stable in the reaction system, is basically not broken and decomposed, can be repeatedly used for multiple times, is more suitable for producing sucrose-6-acetate by using industrial large-scale continuous esterification. The manufacturing method is simple, and the cost is low.
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Inventors:
ZHANG ZHENGSONG (CN)
XU CHUANJIU (CN)
QI FEI (CN)
DAI YONGHUI (CN)
XU CHUANJIU (CN)
QI FEI (CN)
DAI YONGHUI (CN)
Application Number:
PCT/CN2021/071430
Publication Date:
July 21, 2022
Filing Date:
January 13, 2021
Export Citation:
Assignee:
ANHUI JINHE IND CO LTD (CN)
International Classes:
C12N11/10; C12N11/04; C12P19/12
Foreign References:
| CN112888782A | 2021-06-01 | |||
| CN107858343A | 2018-03-30 | |||
| CN104651345A | 2015-05-27 | |||
| CN109575090A | 2019-04-05 | |||
| CN111575327A | 2020-08-25 |
Other References:
YANG XU'E, ET AL.: "Immobilization of Lipozyme TL 100 L and catalytic synthesis of sucrose-6-acetate", CHEMICAL INDUSTRY AND ENGINEERING PROGRESS, vol. 30, no. 11, 12 November 2011 (2011-11-12), pages 2517 - 2522, XP055949962, ISSN: 1000-6613, DOI: 10.16085/j.issn.1000-6613.2011.11.001
XUE YANG; PU ZHENG; YE NI; ZHIHAO SUN;: "Highly efficient biosynthesis of sucrose-6-acetate with cross-linked aggregates of Lipozyme TL 100 L", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 161, no. 1, 11 May 2012 (2012-05-11), Amsterdam NL , pages 27 - 33, XP028433160, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2012.05.014
LI QUNLIANG, ZHANG XIN, GUO XIAOBO, YAO PINGJIA, WEI YUANAN: "Enzymatic Synthesis of Sucrose-6-acetate by a Novel Immobilized Fructosyltransferase From Aspergillus sp. GX-0010", IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 16, no. 2, 15 May 2018 (2018-05-15), pages 114 - 119, XP055949963, DOI: 10.21859/ijb.1380
ZHONG XIANG; QIAN JUNQING; GUO HUI; HU YUANYUAN; LIU MIN: "Biosynthesis of sucrose-6-acetate catalyzed by surfactant-coatedCandida rugosalipase immobilized on sol-gel supports", BIOPROCESS AND BIOSYSTEMS ENGINEERING, SPRINGER, DE, vol. 37, no. 5, 14 September 2013 (2013-09-14), DE , pages 813 - 818, XP035315934, ISSN: 1615-7591, DOI: 10.1007/s00449-013-1053-9
XUE YANG; PU ZHENG; YE NI; ZHIHAO SUN;: "Highly efficient biosynthesis of sucrose-6-acetate with cross-linked aggregates of Lipozyme TL 100 L", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 161, no. 1, 11 May 2012 (2012-05-11), Amsterdam NL , pages 27 - 33, XP028433160, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2012.05.014
LI QUNLIANG, ZHANG XIN, GUO XIAOBO, YAO PINGJIA, WEI YUANAN: "Enzymatic Synthesis of Sucrose-6-acetate by a Novel Immobilized Fructosyltransferase From Aspergillus sp. GX-0010", IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 16, no. 2, 15 May 2018 (2018-05-15), pages 114 - 119, XP055949963, DOI: 10.21859/ijb.1380
ZHONG XIANG; QIAN JUNQING; GUO HUI; HU YUANYUAN; LIU MIN: "Biosynthesis of sucrose-6-acetate catalyzed by surfactant-coatedCandida rugosalipase immobilized on sol-gel supports", BIOPROCESS AND BIOSYSTEMS ENGINEERING, SPRINGER, DE, vol. 37, no. 5, 14 September 2013 (2013-09-14), DE , pages 813 - 818, XP035315934, ISSN: 1615-7591, DOI: 10.1007/s00449-013-1053-9
Attorney, Agent or Firm:
BEIJING LONGAN LAW FIRM (CN)
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