Title:
IMMUNOASSAY METHOD AND ASSAY REAGENT USED IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2016/136917
Kind Code:
A1
Abstract:
[Problem] The purpose of the present invention is to reduce the possibility of nonspecific reactions occurring during the immunoassay of L-FABPs, and provide an assay method that is more accurate and has good sensitivity. More specifically, the purpose of the present invention is to provide an assay method that is sensitive and accurate, even when the concentration of a substance to be assayed is low (for example, when the concentration of L-FABPs is near normal levels). [Solution] A L-FABP immunoassay method using anti-L-FABP antibodies, wherein it is possible to easily suppress nonspecific reactions even during sensitive assays by including a polypeptide comprising the amino acid sequence represented by SEQ ID NO: 1, which is the sequence from the 419th amino acid to the 607th amino acid in the amino acid sequence of the heat shock protein (HSP) DnaK from Escherichia coli, or by including a polypeptide having a sequence identity of at least 90% therewith.
Inventors:
KOBAYASHI KOJI (JP)
MATSUMOTO TAKUJI (JP)
YAMAMOTO MITSUAKI (JP)
MATSUMOTO TAKUJI (JP)
YAMAMOTO MITSUAKI (JP)
Application Number:
PCT/JP2016/055728
Publication Date:
September 01, 2016
Filing Date:
February 25, 2016
Export Citation:
Assignee:
SEKISUI MEDICAL CO LTD (JP)
International Classes:
G01N33/531; G01N33/53; G01N33/543
Domestic Patent References:
| WO2007074860A1 | 2007-07-05 | |||
| WO2005003155A1 | 2005-01-13 |
Foreign References:
| JPH08211056A | 1996-08-20 | |||
| JPH11242026A | 1999-09-07 | |||
| JPH05322891A | 1993-12-07 |
Other References:
KENJIRO KIMURA: "L-FABP no Rinshoteki Igi to Kongo no Tenbo", JAPANESE JOURNAL OF CLINICAL LABORATORY AUTOMATION, vol. 39, no. 4, 2014, pages 433, XP009505458
NOBUHIRO SATO: "Nyo-chu L-FABP no Sokuji Sokutei o Kano ni suru Shinki Latex-ho Shiyaku Norudia L-FABP no Kisoteki Seino Hyoka", JAPANESE JOURNAL OF CLINICAL LABORATORY AUTOMATION, vol. 40, no. 4, 1 September 2015 (2015-09-01), pages 523 269, XP009505460
See also references of EP 3264084A4
NOBUHIRO SATO: "Nyo-chu L-FABP no Sokuji Sokutei o Kano ni suru Shinki Latex-ho Shiyaku Norudia L-FABP no Kisoteki Seino Hyoka", JAPANESE JOURNAL OF CLINICAL LABORATORY AUTOMATION, vol. 40, no. 4, 1 September 2015 (2015-09-01), pages 523 269, XP009505460
See also references of EP 3264084A4
Attorney, Agent or Firm:
MOEGI PATENT OFFICE (JP)
Patent business corporation It burns and is ぎ特許事務所. (JP)
Patent business corporation It burns and is ぎ特許事務所. (JP)
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