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Title:
IMPROVED SLEEPING BEAUTY TRANSPOSON SYSTEM AND GENE TRANSPOSITION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/019400
Kind Code:
A1
Abstract:
In the present invention, an existing SB transposon system was applied to a CHO cell, and higher protein expression than a normal vector was confirmed. A modification was made by further adding a pair of ITRs to existing SB transposon vector ITRs to create a new vector. The new vector showed an improved protein expression profile and resulted in higher stability compared to the existing SB transposon. Also, an increase in the protein expression was confirmed when DNA methylation was inhibited in the CHO cell to which the SB transposon system has been applied.

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Inventors:
PARK JOON TAE (KR)
LEE YUN HAENG (KR)
Application Number:
PCT/KR2020/017709
Publication Date:
January 27, 2022
Filing Date:
December 07, 2020
Export Citation:
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Assignee:
INU RES & BUSINESS FOUNDATION (KR)
International Classes:
C12N15/90
Foreign References:
US20180135032A12018-05-17
US20060205081A12006-09-14
US20050112764A12005-05-26
KR20180127358A2018-11-28
Other References:
HATEM ZAYED, ZSUZSANNA IZSVAK, OLIVER WALISKO, ZOLTAN IVICS: "Development of hyperactive sleeping beauty transposon vectors by mutational analysis.", MOLECULAR THERAPY, ELSEVIER INC., US, vol. 9, no. 2, 1 February 2004 (2004-02-01), US , pages 292 - 304, XP008087829, ISSN: 1525-0016, DOI: 10.1016/j.ymthe.2003.11.024
Attorney, Agent or Firm:
CHA, Joon Yong (KR)
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