IN VIVO HIGH THROUGHPUT SELECTION OF EFFECTIVE RNAi PROBES

WIPO Patent Application WO2004111190

A3

In mammalian systems, RNA interference (RNAi)-based suppression of target gene expression may be activated by delivery of RNAi probes such as double stranded small interfering RNA (siRNA) molecules or short hairpin RNAs (shRNAs), where the RNAi probe sequence is homologous to the target gene. A reliable and quantitative method is provided for the rapid and efficient identification of RNAi probes that are most effective in providing RNAi-mediated suppression of target gene expression. This method may be used for high-throughput screens to identify effective RNAi probes.

JP2664892	[Title of the Invention] Herpes simplex virus as a vector
WO/2000/027864	CHROMOSOME 17p-LINKED PROSTATE CANCER SUSCEPTIBILITY GENE

MITTAL VIVEK (US)
KUMAR RAJEEV (US)

PCT/US2004/016844

April 14, 2005

May 26, 2004

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COLD SPRING HARBOR LAB (US)
MITTAL VIVEK (US)
KUMAR RAJEEV (US)

C07H21/04; C12N15/63; C12Q1/68; C12N; (IPC1-7): C07H21/04; C12N15/63

CHIU Y.L. ET AL.: "RNAi in human cells: basic structural and functional feature s of small interferring RNA", MOLECULAR CELL., vol. 10, September 2002 (2002-09-01), pages 549 - 561, XP002978510
YU ET AL.: "RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells", PNAS, vol. 99, 30 April 2002 (2002-04-30), pages 6047 - 6052, XP002297663
BAILEY ET AL.: "Applications of transfected cell microarrays in high-throughput drug discovery", DRUG DISCOV. TODAY, vol. 7, no. 18, 2002, pages S113 - S118, XP008026706

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