Title:
METHODS AND COMPOSITIONS FOR CONTROLLING EFFICACY OF RNA SILENCING
Document Type and Number:
WIPO Patent Application WO2004111191
Kind Code:
A3
Abstract:
Based at least in part on an understanding of the mechanisms by which small RNAs (e.g., naturally-occurring miRNAs) mediate RNA silencing in plants, rules have been established for determining, for example, the degree of complementarity required between an RNAi-mediating agent and its target, i.e., whether mismatches are tolerated, the number of mismatches tolerated, the effect of the position of the mismatches, etc. Such rules are useful, in particular, in the design of improved RNAi-mediating agents which allow for more exact control of the efficacy of RNA silencing.
Inventors:
ZAMORE PHILLIP D (US)
TANG GUILIANG (US)
TANG GUILIANG (US)
Application Number:
PCT/US2004/017130
Publication Date:
December 22, 2005
Filing Date:
June 02, 2004
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
ZAMORE PHILLIP D (US)
TANG GUILIANG (US)
ZAMORE PHILLIP D (US)
TANG GUILIANG (US)
International Classes:
A61K48/00; C07H21/00; C12N15/11; C12N15/82; C12N; (IPC1-7): C07H21/00
Domestic Patent References:
| WO2001075164A2 | 2001-10-11 |
Foreign References:
| US20040137471A1 | 2004-07-15 |
Other References:
ELBASHIR S.M. ET AL: "Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate", THE EMBO JOURNAL, vol. 20, no. 23, 3 December 2001 (2001-12-03), pages 6877 - 6888, XP002225998
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