Title:
ISOLATION METHOD OF FETAL-DERIVED HEMATOPOIETIC PROGENITOR CELLS, AND METHOD FOR TESTING POSSIBILITY OF CHROMOSOMAL ABNORMALITY OF FETUS
Document Type and Number:
WIPO Patent Application WO/2018/198597
Kind Code:
A1
Abstract:
To provide a preparation method of a new sample which, without requiring a definite diagnosis using a biopsy, can be used as a liquid biopsy sample in prenatal diagnosis of a fetus. This isolation method of fetal-derived hematopoietic progenitor cells is characterized by involving an emulsion forming step, a culturing step, a detection step and a selection step. In the emulsion forming step, a blood sample of the pregnant woman, a culture medium, and an emulsion forming solvent are brought into contact to form, in the emulsion forming solvent, multiple droplets that contain the pregnant woman-derived hematopoietic progenitor cells or fetus-derived hematopoietic progenitor cells contained in the blood sample. In the culturing step, the aforementioned multiple droplets are cultured in the presence of a growth factor of hematopoietic progenitor cells. In the detection step, the fetus-derived hematopoietic progenitor cells are detected in the droplets after culturing. In the selection step, droplets in which the fetus-derived hematopoietic progenitor cells have been detected are selected from the droplets after culturing.
Inventors:
SUNAGA NOBUYA (JP)
Application Number:
PCT/JP2018/010969
Publication Date:
November 01, 2018
Filing Date:
March 20, 2018
Export Citation:
Assignee:
ENPLAS CORP (JP)
International Classes:
C12N5/0789; C12Q1/02; C12Q1/6827; C12Q1/6844; G01N1/10; G01N33/48; A61B5/154; A61J1/05
Domestic Patent References:
| WO2013188828A1 | 2013-12-19 | |||
| WO2017217323A1 | 2017-12-21 |
Foreign References:
| JPH11500010A | 1999-01-06 | |||
| JP2008526247A | 2008-07-24 | |||
| JP2014521334A | 2014-08-28 |
Attorney, Agent or Firm:
TSUJIMARU Koichiro et al. (JP)
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