Title:
ISOLATION AND PURIFICATION METHOD OF EXTRACELLULAR VESICLES
Document Type and Number:
WIPO Patent Application WO/2022/250035
Kind Code:
A1
Abstract:
[Problem] To provide an isolation and purification method for isolating and purifying extracellular vesicles. [Solution] An isolation and purification method of extracellular vesicles, said method being for obtaining a concentrated solution having an increased concentration of extracellular vesicles by a filtration process using a hollow fiber membrane, wherein: the hollow fiber membrane has an inner diameter of 0.2-1.4 mm and a fractional molecular weight of 100,000-1,000,000; the filtration process uses alternating tangential flow filtration, which includes a first filtration step for pressurizing a mesenchymal stem cell culture supernatant solution through a first opening at one end of the hollow fiber membrane, filtering and thus separating into a permeate and a first concentrated solution, and a second filtration step for pressurizing the first concentrated solution through a second opening at the other end of the hollow fiber membrane, filtering and thus separating into a permeate and a second concentrated solution, and in which the first and second filtration steps are alternately performed multiple times; and the membrane surface velocity in the first and second filtration steps is 0.3-2 m/sec.
Inventors:
NAKATSUKA SHUJI (JP)
UCHIMURA SEIICHI (JP)
OCHIYA TAKAHIRO (JP)
YOSHIOKA YUSUKE (JP)
UCHIMURA SEIICHI (JP)
OCHIYA TAKAHIRO (JP)
YOSHIOKA YUSUKE (JP)
Application Number:
PCT/JP2022/021193
Publication Date:
December 01, 2022
Filing Date:
May 24, 2022
Export Citation:
Assignee:
DAICEN MEMBRANE SYSTEMS LTD (JP)
DAICEL CORP (JP)
DAICEL CORP (JP)
International Classes:
C12N1/00; B01D61/14; B01D61/58; B01D63/02; B01D69/00; B01D69/02; B01D69/08; C07K1/34; C12M1/12; C12N5/0775
Foreign References:
| JP2018076291A | 2018-05-17 | |||
| JP2020526190A | 2020-08-31 | |||
| US20190276790A1 | 2019-09-12 | |||
| JP2015530110A | 2015-10-15 | |||
| JPH0339084B2 | 1991-06-12 | |||
| JP2018076291A | 2018-05-17 | |||
| JP2011067175A | 2011-04-07 | |||
| JP2003052360A | 2003-02-25 |
Other References:
YUSUKE YOSHIOKATAKAHIRO OCHIYA: "Exosome provides new insight into liquid biopsy", CYTOMETRY RESEARCH, vol. 26, no. 1, 2016, pages 1 - 6
Attorney, Agent or Firm:
FURUYA Satoshi (JP)
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