Title:
KNOWN MOLECULE AND PROTEIN INTERACTION DETECTION SYSTEM BASED ON COVALENT LINKAGE, AND IDENTIFICATION OR VERIFICATION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/155986
Kind Code:
A1
Abstract:
Disclosed are a known molecule and protein interaction detection system based on covalent linkage, and an identification or verification method thereof. The detection system comprises: a) a streptavidin-short peptide tetramer; b) a PafA enzyme; and c) a biotin-modified known molecule. The present method uses biotin to mark the known molecule, after the known molecule interacts with a protein, efficiently captures, under a mild condition by means of the streptavidin-short peptide tetramer, the protein interacting with the known molecule, and under the catalysis of the PafA enzyme, covalently links a short peptide to the protein that interacts with the known molecule, thereby converting the non-covalent bonding between the known molecule and the protein interacting with each other into the covalent linkage between streptavidin and the protein, and thereby performing analysis, separation, and identification.
Inventors:
TAO SHENGCE (CN)
JIANG HEWEI (CN)
ZHENG YUNXIAO (CN)
CHEN HONG (CN)
WANG XUENING (CN)
JIANG HEWEI (CN)
ZHENG YUNXIAO (CN)
CHEN HONG (CN)
WANG XUENING (CN)
Application Number:
PCT/CN2021/073899
Publication Date:
July 28, 2022
Filing Date:
January 27, 2021
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
G01N33/68
Foreign References:
| US20160377607A1 | 2016-12-29 | |||
| CN108486218A | 2018-09-04 | |||
| CN106093436A | 2016-11-09 | |||
| CN110669109A | 2020-01-10 |
Other References:
LIU QIANG; ZHENG JUN; SUN WEIPING; HUO YINBO; ZHANG LIYE; HAO PILIANG; WANG HAOPENG; ZHUANG MIN: "A proximity-tagging system to identify membrane protein–protein interactions", NATURE METHODS, vol. 15, no. 9, 13 August 2018 (2018-08-13), New York, pages 715 - 722, XP036953339, ISSN: 1548-7091, DOI: 10.1038/s41592-018-0100-5
Attorney, Agent or Firm:
DUAN&DUAN LAW FIRM (CN)
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