Title:
LIPASE ACTIVITY MEASUREMNET METHOD AND REAGENT, AND SUBSTRATE SOLUTION FOR USE IN MEASUREMENT OF ACTIVITY OF LIPASE
Document Type and Number:
WIPO Patent Application WO/2018/056162
Kind Code:
A1
Abstract:
[Problem] To provide: a substrate solution for use in the measurement of the activity of a lipase, which contains 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin)-ester [DGGMR] as a lipase activity measurement substrate, and in which the deterioration of DGGMR can be prevented and the decrease in an absorbance value obtained by the measurement can be prevented even when the solution is contaminated by an azide; a lipase activity measurement reagent; a lipase activity measurement method; and a method for reducing the influence of an azide.
[Solution] A substrate solution for use in the measurement of the activity of a lipase, said substrate solution containing 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin)-ester [DGGMR] as a lipase activity measurement substrate, wherein a reducing agent is further contained in the substrate solution.
Inventors:
IIZUKA NAOMI (JP)
HIKICHI ATSUSHI (JP)
HIKICHI ATSUSHI (JP)
Application Number:
PCT/JP2017/033234
Publication Date:
March 29, 2018
Filing Date:
September 14, 2017
Export Citation:
Assignee:
SHINO TEST CORP (JP)
International Classes:
C12Q1/44
Domestic Patent References:
| WO2016024549A1 | 2016-02-18 |
Foreign References:
| JPS61254197A | 1986-11-11 | |||
| JPH11318494A | 1999-11-24 |
Other References:
PANTEGHINI M. ET AL.: "Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate", ANN. CLIN. BIOCHEM., vol. 38, no. 4, 2001, pages 365 - 370, XP008073430, DOI: doi:10.1258/0004563011900876
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