Title:
LIQUID CHROMATOGRAPHIC ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2016/129322
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a liquid chromatographic analysis method whereby a hydrophobic compound and a hydrophilic compound can be easily analyzed in a single column without using a plurality of analytical columns or analyzers. Provided is a liquid chromatographic analysis method characterized by comprising: using an analytical column packed with a polymer packing having a hydroxyl group; using as a mobile phase a solvent mixture consisting of a water-soluble organic solvent with water; and switching a reversed phase chromatography mode and a hydrophilic interaction chromatography mode by changing the composition ratio of the water-soluble organic solvent and water in the mobile phase to thereby analyze a hydrophilic compound and a hydrophobic compound.
Inventors:
KONDO HIDEYUKI (JP)
SASUGA JUNJI (JP)
SHUKUYA TAKAYUKI (JP)
SASUGA JUNJI (JP)
SHUKUYA TAKAYUKI (JP)
Application Number:
PCT/JP2016/050978
Publication Date:
August 18, 2016
Filing Date:
January 14, 2016
Export Citation:
Assignee:
SHOWA DENKO KK (JP)
International Classes:
G01N30/88; B01D15/08; B01J20/281; G01N30/26; G01N30/34
Foreign References:
| JP2007093585A | 2007-04-12 | |||
| JP2010529431A | 2010-08-26 | |||
| JP2007057526A | 2007-03-08 | |||
| JP2000187028A | 2000-07-04 |
Other References:
LIU X ET AL.: "New hydrophilic interaction/ reversed-phase mixed-mode stationary phase and its application for analysis of nonionic ethoxylated surfactants", JOURNAL OF CHROMATOGRAPHY A, vol. 1191, no. 1-2, 16 May 2008 (2008-05-16), pages 83 - 89
LI Y ET AL.: "New reversed-phase/anion-exchange/ hydrophilic interaction mixed-mode stationary phase based on dendritic polymer-modified porous silica", JOURNAL OF CHROMATOGRAPHY A, vol. 1337, 11 April 2014 (2014-04-11), pages 133 - 139
JANDERA P: "Stationary and mobile phases in hydrophilic interaction chromatography: a review", ANALYTICA CHIMICA ACTA, vol. 692, no. 1-2, 29 April 2011 (2011-04-29), pages 1 - 25
LI Y ET AL.: "New reversed-phase/anion-exchange/ hydrophilic interaction mixed-mode stationary phase based on dendritic polymer-modified porous silica", JOURNAL OF CHROMATOGRAPHY A, vol. 1337, 11 April 2014 (2014-04-11), pages 133 - 139
JANDERA P: "Stationary and mobile phases in hydrophilic interaction chromatography: a review", ANALYTICA CHIMICA ACTA, vol. 692, no. 1-2, 29 April 2011 (2011-04-29), pages 1 - 25
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
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