Title:
LIVE CELL FREEZING METHOD AND LIVE CELL FREEZING SYSTEM
Document Type and Number:
WIPO Patent Application WO/2023/243097
Kind Code:
A1
Abstract:
Provided are a live cell freezing system and a method for freezing live cells that includes cooling an unfrozen sample that contains a freezing solution containing a cryoprotectant and live cells that are immersed in the freezing solution while allowing a conduction current to flow by placing the sample in an electric field generated from an electrode to which an alternating current voltage has been applied to freeze the unfrozen sample. Also provide is a method for forming spheroids that includes freezing live cells having spheroid formation ability by the freezing method, thawing the frozen live cells, and culturing the thawed live cells to form spheroids.
Inventors:
BAMBA KENZO (JP)
KOHARA ARIHIRO (JP)
OZAWA MIDORI (JP)
MORISHIMA YASUYUKI (JP)
HARA YUKO (JP)
SUMIKAWA HIROAKI (JP)
KOHARA ARIHIRO (JP)
OZAWA MIDORI (JP)
MORISHIMA YASUYUKI (JP)
HARA YUKO (JP)
SUMIKAWA HIROAKI (JP)
Application Number:
PCT/JP2022/024417
Publication Date:
December 21, 2023
Filing Date:
June 17, 2022
Export Citation:
Assignee:
NAT INST BIOMEDICAL INNOVATION HEALTH & NUTRITION (JP)
SANTETSU ENG INC (JP)
LINKSU CO LTD (JP)
SANTETSU ENG INC (JP)
LINKSU CO LTD (JP)
International Classes:
C12N1/04; C12N5/07; C12N5/0735; F25B21/00
Domestic Patent References:
| WO2018185912A1 | 2018-10-11 |
Foreign References:
| JP2007259709A | 2007-10-11 | |||
| JP2011182700A | 2011-09-22 | |||
| JP2006115837A | 2006-05-11 |
Other References:
IMAMURA SHINSUKE: "Cryogenic storage of Primate ES/iPS cells", SEIBUTSU KOGAKU. BIOENGINEERING, vol. 90, 1 January 2012 (2012-01-01), pages 552 - 553, XP093117608
Attorney, Agent or Firm:
MIYOSHI Hidekazu et al. (JP)
Download PDF: