Title:
MDH GENE-BASED POLYNUCLEOTIDE HAVING PROMOTER ACTIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/257758
Kind Code:
A1
Abstract:
A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.
Inventors:
ZHENG PING (CN)
LIU JIAO (CN)
SUN JIBIN (CN)
SUN GUANNAN (CN)
ZHOU WENJUAN (CN)
GUO XUAN (CN)
LIU JIAO (CN)
SUN JIBIN (CN)
SUN GUANNAN (CN)
ZHOU WENJUAN (CN)
GUO XUAN (CN)
Application Number:
PCT/CN2022/094736
Publication Date:
December 15, 2022
Filing Date:
May 24, 2022
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N15/113; C07K14/34; C12N1/20; C12N15/09; C12N15/67; C12R1/06; C12R1/13; C12R1/15; C12R1/185
Domestic Patent References:
| WO2006028063A1 | 2006-03-16 | |||
| WO1993008277A1 | 1993-04-29 |
Foreign References:
| CN103981203A | 2014-08-13 | |||
| CN1440458A | 2003-09-03 |
Other References:
WANG, YINGCHUN ET AL.: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time- series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 11, 25 November 2018 (2018-11-25), pages 1760 - 1771, XP055901944, ISSN: 1000-3061, DOI: 10.13345/j.cjb.180041
VAN DER REST, M. ET AL.: "Functions of the Membrane-Associated and Cytoplasmic Malate Dehydrogenases in the Citric Acid Cycle of Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 182, no. 24, 31 December 2000 (2000-12-31), pages 6892 - 6899, XP002179953, ISSN: 0021-9193, DOI: 10.1128/JB.182.24.6892-6899.2000
"Microbial Metabolic Engineering: Methods and Protocols", 1989, COLD SPRING HARBOR LABORATORY PRESS
WANG, YC ET AL.: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 11, 2018, pages 1760 - 1771, XP055901944, DOI: 10.13345/j.cjb.180041
VAN DER REST, M. ET AL.: "Functions of the Membrane-Associated and Cytoplasmic Malate Dehydrogenases in the Citric Acid Cycle of Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 182, no. 24, 31 December 2000 (2000-12-31), pages 6892 - 6899, XP002179953, ISSN: 0021-9193, DOI: 10.1128/JB.182.24.6892-6899.2000
"Microbial Metabolic Engineering: Methods and Protocols", 1989, COLD SPRING HARBOR LABORATORY PRESS
WANG, YC ET AL.: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 11, 2018, pages 1760 - 1771, XP055901944, DOI: 10.13345/j.cjb.180041
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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