Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
MEASUREMENT METHOD FOR APP CLEAVAGE PEPTIDES
Document Type and Number:
WIPO Patent Application WO/2015/111430
Kind Code:
A1
Abstract:
　Provided is a measurement method for amyloid precursor protein (APP) cleavage peptides including amyloid beta (Aβ) peptides. This method for measuring APP cleavage peptides in a blood sample includes: a step for bringing a blood sample into contact, within a binding solution, with an antibody-immobilizing carrier that includes a carrier and an antibody bonded to the carrier and selected from the group consisting of immunoglobulins having antigen-binding sites capable of recognizing APP cleavage peptides, and immunoglobulin fragments containing antigen-binding sites capable of recognizing APP cleavage peptides, and bringing about binding of the antibody-immobilizing carrier and APP cleavage peptides contained in the blood sample; a step for washing the bound antibody-immobilizing carrier and APP cleavage peptides with a washing solution; a step for dissociating the APP cleavage peptides from the antibody-immobilizing carrier, using an acidic aqueous solution containing an organic solvent; and a step for detecting the dissociated APP cleavage peptides.

More Like This:
WO/2009/017405PROTEIN MARKERS FOR CARDIOVASCULAR EVENTS
WO/2005/015209METHODS AND SYSTEMS FOR THE ANNOTATION OF BIOMOLECULE PATTERNS IN CHROMATOGRAPHY/MASS-SPECTROMETRY ANALYSIS
WO/2022/221876DIGITAL PROTEIN MISFOLDING ASSAYS
Inventors:
KANEKO NAOKI (JP)
Application Number:
PCT/JP2015/050255
Publication Date:
July 30, 2015
Filing Date:
January 07, 2015
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
SHIMADZU CORP (JP)
International Classes:
G01N33/68; G01N27/62
Domestic Patent References:
WO2008065806A12008-06-05
WO2009057664A12009-05-07
Foreign References:
JP2010515717A2010-05-13
JP2014020872A2014-02-03
Other References:
KANEKO,N: "Multi Epitope-Targeting Immunoprecipitation Using F(ab') Fragments with High Affinity and Specificity for the Enhanced Detection of a Peptide with Matrix- Assisted Laser Desorption Ionization-Time-of- Flight Mass Spectrometry", ANAL. CHEM., vol. 85, no. 6, 19 March 2013 (2013-03-19), pages 3152 - 3159, XP055104533
Attorney, Agent or Firm:
OKADA, MASAHIRO (JP)
Masahiro Okada (JP)
Download PDF:
View/Download PDF      PDF Help



 
Previous Patent: COATED ARTICLE, AND ANTICORROSIVE COATING FORMING METHOD

Next Patent: STERILIZED WORK SYSTEM