Title:
MEDIUM FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, METHOD FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, AND METHOD FOR PRODUCING RENAL ORGANOIDS
Document Type and Number:
WIPO Patent Application WO/2022/149616
Kind Code:
A1
Abstract:
A medium for culturing and expanding nephron progenitor cells, said medium containing a GSK-3β inhibitor, a ROCK inhibitor and at least one fibroblast growth factor selected from the group consisting of FGF9 and FGF20. A method for culturing and expanding nephron progenitor cells using the aforesaid medium. A method for producing renal organoids, said method comprising a step for culturing and expanding nephron progenitor cells by the aforesaid method for culturing and expanding nephron progenitor cells, and a step for differentiating the nephron progenitor cells, which have been thus cultured and expanded, into renal organoids.
Inventors:
OSAFUNE KENJI (JP)
ARAOKA TOSHIKAZU (JP)
KOBAYASHI YOSHIFUMI (JP)
RYOSAKA MAKOTO (JP)
TSUJIMOTO HIRAKU (JP)
ARAOKA TOSHIKAZU (JP)
KOBAYASHI YOSHIFUMI (JP)
RYOSAKA MAKOTO (JP)
TSUJIMOTO HIRAKU (JP)
Application Number:
PCT/JP2022/000564
Publication Date:
July 14, 2022
Filing Date:
January 11, 2022
Export Citation:
Assignee:
UNIV KYOTO (JP)
REGE NEPHRO CO LTD (JP)
REGE NEPHRO CO LTD (JP)
International Classes:
C12N5/077; C12N5/10
Domestic Patent References:
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| WO2021125340A1 | 2021-06-24 | |||
| WO2020213734A1 | 2020-10-22 | |||
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| WO2014200115A1 | 2014-12-18 | |||
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TANENAGA, K. ET AL., CANCER RES., vol. 40, 1980, pages 914 - 919
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P. B. YU ET AL., NAT. CHEM. BIOL., vol. 4, 2008, pages 33 - 41
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NAT BIOTECHNOL, vol. 28, 2010, pages 611 - 615
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J. CELL BIOL., vol. 105, 1987, pages 589 - 598
NATURE, vol. 526, 2015, pages 564 - 568
DEV CELL, vol. 23, no. 3, 11 September 2012 (2012-09-11), pages 637 - 651
C.A. SCHNEIDER ET AL., NAT METHODS., vol. 9, 2012, pages 671 - 675
OSAFUNE K. ET AL.: "Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony-forming assay", DEVELOPMENT, vol. 133, 2006, pages 151 - 61, XP002581530, DOI: 10.1242/dev.02174
KOBAYASHI A. ET AL.: "Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development", CELL STEM CELL, vol. 3, 2008, pages 169 - 81, XP055078817, DOI: 10.1016/j.stem.2008.05.020
Z. LIT. ARAOKAJ.C.I. BELMONTE: "Kidney Organog Methods Protoc", 2019, HUMMANA PRESS, article "Gene Editing in 3D Cultured Nephron Progenitor Cell Lines", pages: 151 - 159
OSAFUNE K ET AL., DEVELOPMENT, vol. 133, 2006, pages 151 - 61
CELL STEM CELL, vol. 3, 2008, pages 169 - 181
NAT COMMUN, vol. 4, pages 1367
"NCBI", Database accession no. NP_062825.1
ISHIZAKI ET AL., MOL. PHARMACOL., vol. 57, 2000, pages 976 - 983
NARUMIYA ET AL., METHODS ENZYMOL., vol. 325, 2000, pages 273 - 284
UENATA ET AL., NATURE, vol. 389, 1997, pages 990 - 994
SASAKI ET AL., PHARMACOL. THER., vol. 93, 2002, pages 225 - 232
NAKAJIMA ET AL., CANCER CHEMOTHER PHARMACOL., vol. 52, no. 4, 2003, pages 319 - 324
BARAK, H. ET AL.: "FGF9 and FGF20 Maintain the Sternness of Nephron Progenitors in Mice and Man", DEV. CELL, vol. 22, 2012, pages 1191 - 1207, XP028490953, DOI: 10.1016/j.devcel.2012.04.018
CAS , no. 1187594-09-7
CAS , no. 58753-54-1
TAMURA K ET AL., CELL DIFFER. DEV., vol. 32, 1990, pages 17 - 26
STRICKLAND S ET AL., CANCER RES., vol. 43, 1983, pages 5268 - 5272
TANENAGA, K. ET AL., CANCER RES., vol. 40, 1980, pages 914 - 919
LINDEMANN ET AL., MOL. CANCER, vol. 2, 2003, pages 20
P. B. YU ET AL., CIRCULATION, vol. 116, 2007, pages 60
P. B. YU ET AL., NAT. CHEM. BIOL., vol. 4, 2008, pages 33 - 41
J. HAO ET AL., PLOS ONE, vol. 3, no. 8, 2008, pages e2904
NAT BIOTECHNOL, vol. 28, 2010, pages 611 - 615
EMBO J., vol. 3, 1984, pages 1463 - 1468
J. CELL BIOL., vol. 105, 1987, pages 589 - 598
NATURE, vol. 526, 2015, pages 564 - 568
DEV CELL, vol. 23, no. 3, 11 September 2012 (2012-09-11), pages 637 - 651
C.A. SCHNEIDER ET AL., NAT METHODS., vol. 9, 2012, pages 671 - 675
Attorney, Agent or Firm:
TAZAKI Akira et al. (JP)
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