Title:
MELITTIN-BASED NANOPARTICLE COMPOSITE AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2023/249386
Kind Code:
A1
Abstract:
The present invention relates to a melittin-based nanoparticle composite and a method for preparing same, in which, by combining melittin and a hydrophobic polymer to form nanoparticles through self-assembly, and then coating the outermost side of the nanoparticles by using biomaterials such as albumin and hyaluronic acid through electrostatic attraction, a nanoparticle composite is formed, and thus, the toxicity of melittin itself can be reduced, cancer cells can be effectively killed, and the expression of inflammation-related factors can be inhibited, and since the hydrophobic polymer binds to the C-terminal of melittin through a disulfide bond, after uptake into cancer cells or immune cells, biomaterials such as albumin and hyaluronic acid are removed, and then the disulfide bond is broken down by glutathione present in the cells and melittin is released, and thus, an anticancer or anti-inflammatory effect can be further enhanced. Details of the project that supported this invention are as follows. [Project identification number]202131038[Project number]NRF-2020R1A2B5B02001733[Ministry name]Ministry of Science and ICT[Project management (professional) organization name]National Research Foundation of Korea[Research project name]Mid-career researcher support project[Research project name]Improvement of stability of ultrasound contrast media and maximization of chemotherapy and photodynamic therapy efficiency through exosome fusion[Contribution rate]0.5/0.5[Name of organization carrying out project]Sogang University Industry-Academic Cooperation Foundation[Research period]01 March 2021-28 February 2022 Details of another project that supported this invention are as follows. [Project identification number]202121001[Project number]NRF-2016R1A6A1A03012845[Ministry name]Ministry of Education [Project management (professional) organization name]National Research Foundation of Korea[Research project name]Key research institute support project[Research project name]Development of nanobiochip with function of evaluating brain disease drug[Contribution rate]0.5/0.5[Name of organization carrying out project]Sogang University Industry-Academic Cooperation Foundation[Research period]01 January 2021-31 December 2021
Inventors:
KIM HYUN CHEOL (KR)
HAN HYOUN KOO (KR)
CHO YOUNG HEUN (KR)
HAN EUN KYUNG (KR)
YOO IN SOO (KR)
NOH SEUNG KWON (KR)
PARK JUNG SOO (KR)
NAM DO HYUN (KR)
PARK SU HYEON (KR)
KIM DO YEON (KR)
HAN HYOUN KOO (KR)
CHO YOUNG HEUN (KR)
HAN EUN KYUNG (KR)
YOO IN SOO (KR)
NOH SEUNG KWON (KR)
PARK JUNG SOO (KR)
NAM DO HYUN (KR)
PARK SU HYEON (KR)
KIM DO YEON (KR)
Application Number:
PCT/KR2023/008555
Publication Date:
December 28, 2023
Filing Date:
June 20, 2023
Export Citation:
Assignee:
SOGANG UNIV RESEARCH ＆ BUSINESS DEVELOPMENT FOUNDATION (KR)
INSBIOPHARM CO LTD (KR)
INSBIOPHARM CO LTD (KR)
International Classes:
A61K47/69; A61K9/107; A61K38/17; A61K47/24; A61K47/34; A61K47/36; A61K47/42; A61P35/00
Foreign References:
| KR20150129873A | 2015-11-23 |
Other References:
BEI CHENG, BINDU THAPA, REMANT K. C., PEISHENG XU: "Dual secured nano-melittin for the safe and effective eradication of cancer cells", JOURNAL OF MATERIALS CHEMISTRY. B, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 3, no. 1, 1 January 2015 (2015-01-01), GB , pages 25 - 29, XP055363452, ISSN: 2050-750X, DOI: 10.1039/C4TB01401D
WANG YONGJUN, LIU DAN, ZHENG QINGCHUAN, ZHAO QIANG, ZHANG HONGJUAN, MA YAN, FALLON JOHN K., FU QIANG, HAYNES MATTHEW T., LIN GUIME: "Disulfide Bond Bridge Insertion Turns Hydrophobic Anticancer Prodrugs into Self-Assembled Nanomedicines", NANO LETTERS, AMERICAN CHEMICAL SOCIETY, US, vol. 14, no. 10, 8 October 2014 (2014-10-08), US , pages 5577 - 5583, XP093121305, ISSN: 1530-6984, DOI: 10.1021/nl502044x
HAN, EUNKYUNG, KIM HYUNCHEOL: "PSP25: Development of Polymersomes Co-deliverying Dual-drug for Overcoming Multidrug Resistance.", 2021 KSBB FALL MEETING AND INTERNATIONAL SYMPOSIUM; OCTOBER 6-9, 2021, KOREAN SOCIETY FOR BIOTECHNOLOGY AND BIOENGINEERING, KOREA, 1 January 2021 (2021-01-01) - 9 October 2021 (2021-10-09), Korea, pages 217, XP009552193
LINLIN YANG, FUDE CUI, KAI SHI, DONGMEI CUN, RUI WANG: "Design of high payload PLGA nanoparticles containing melittin/sodium dodecyl sulfate complex by the hydrophobic ion-pairing technique", JOURNAL DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, NEW YORK, NY, US, vol. 35, no. 8, 1 August 2009 (2009-08-01), US , pages 959 - 968, XP055618847, ISSN: 0363-9045, DOI: 10.1080/03639040902718039
WANG YONGJUN, LIU DAN, ZHENG QINGCHUAN, ZHAO QIANG, ZHANG HONGJUAN, MA YAN, FALLON JOHN K., FU QIANG, HAYNES MATTHEW T., LIN GUIME: "Disulfide Bond Bridge Insertion Turns Hydrophobic Anticancer Prodrugs into Self-Assembled Nanomedicines", NANO LETTERS, AMERICAN CHEMICAL SOCIETY, US, vol. 14, no. 10, 8 October 2014 (2014-10-08), US , pages 5577 - 5583, XP093121305, ISSN: 1530-6984, DOI: 10.1021/nl502044x
HAN, EUNKYUNG, KIM HYUNCHEOL: "PSP25: Development of Polymersomes Co-deliverying Dual-drug for Overcoming Multidrug Resistance.", 2021 KSBB FALL MEETING AND INTERNATIONAL SYMPOSIUM; OCTOBER 6-9, 2021, KOREAN SOCIETY FOR BIOTECHNOLOGY AND BIOENGINEERING, KOREA, 1 January 2021 (2021-01-01) - 9 October 2021 (2021-10-09), Korea, pages 217, XP009552193
LINLIN YANG, FUDE CUI, KAI SHI, DONGMEI CUN, RUI WANG: "Design of high payload PLGA nanoparticles containing melittin/sodium dodecyl sulfate complex by the hydrophobic ion-pairing technique", JOURNAL DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, NEW YORK, NY, US, vol. 35, no. 8, 1 August 2009 (2009-08-01), US , pages 959 - 968, XP055618847, ISSN: 0363-9045, DOI: 10.1080/03639040902718039
Attorney, Agent or Firm:
KIM, Hyun Jin (KR)
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