Title:
MEMBRANE PROTEIN ACTIVITY MEASUREMENT METHOD
Document Type and Number:
WIPO Patent Application WO/2020/026979
Kind Code:
A1
Abstract:
The present invention provides a comprehensive assay capable of comprehensively measuring interactors for various different types of proteins. The present invention provides a system for detecting the activation of a protein by integrating changes in the transcription regulatory region of a gene the expression of which is changed by activation of the protein via a plurality of factors. The stimulation response ratio of transcription activity before and after stimulation by ligands of each transcription regulatory region is synergistically integrated to permit construction of a highly sensitive reporter assay system.
More Like This:
Inventors:
IKEDA YUICHI (JP)
KUMAGAI HIDETOSHI (JP)
KUMAGAI HIDETOSHI (JP)
Application Number:
PCT/JP2019/029424
Publication Date:
February 06, 2020
Filing Date:
July 26, 2019
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12N15/63; C07K14/705; C12N5/10; C12N15/62; C12Q1/06; C12Q1/66
Domestic Patent References:
| WO2001055726A1 | 2001-08-02 |
Foreign References:
| JP2016149942A | 2016-08-22 | |||
| JP2006507817A | 2006-03-09 | |||
| US20130198901A1 | 2013-08-01 | |||
| JP2013513389A | 2013-04-22 | |||
| JP4968498B2 | 2012-07-04 | |||
| JP2013128413A | 2013-07-04 |
Other References:
LATCHMANDS, INT. J. BIOCHEM. CELL BIOL., vol. 29, no. 12, 1997, pages 1305 - 12
LEE TIYOUNG RA: "which is incorporated herein by reference in its entirety. References are made to Annu", REV. GENET., vol. 34, 2000, pages 77 - 137
MITCHELL PJTJIAN R, SCIENCE, vol. 245, no. 4916, 1989, pages 371 - 8
MARK ET AL., PROC. NATL ACAD SCI USA, vol. 81, no. 18, September 1984 (1984-09-01), pages 5566 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES., vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, pages 1431 - 1433
NAT BIOTECHNOL, vol. 30, 2012, pages 460 - 465
NUCLEIC ACIDS RES, vol. 39, 2011, pages e82
NAT BIOTECHNOL, vol. 20, 2002, pages 135 - 141
MOL CELL, vol. 31, 2008, pages 294 - 301
NAT METHODS, vol. 8, 2011, pages 67 - 69
NAT BIOTECHNOL, vol. 26, 2008, pages 695 - 701
COLD SPRING HARB PROTOC., vol. 2015, no. 11, November 2015 (2015-11-01), pages 951 - 969
SUZUKI K ET AL., NATURE, vol. 540, 2016, pages 144 - 149
CONKLIN BR ET AL., NATURE, vol. 363, 1993, pages 274 - 276
CONKLIN BR ET AL., MOL. PHARMACOLOGY, vol. 50, 1996, pages 885 - 890
LEE TIYOUNG RA: "which is incorporated herein by reference in its entirety. References are made to Annu", REV. GENET., vol. 34, 2000, pages 77 - 137
MITCHELL PJTJIAN R, SCIENCE, vol. 245, no. 4916, 1989, pages 371 - 8
MARK ET AL., PROC. NATL ACAD SCI USA, vol. 81, no. 18, September 1984 (1984-09-01), pages 5566 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES., vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, pages 1431 - 1433
NAT BIOTECHNOL, vol. 30, 2012, pages 460 - 465
NUCLEIC ACIDS RES, vol. 39, 2011, pages e82
NAT BIOTECHNOL, vol. 20, 2002, pages 135 - 141
MOL CELL, vol. 31, 2008, pages 294 - 301
NAT METHODS, vol. 8, 2011, pages 67 - 69
NAT BIOTECHNOL, vol. 26, 2008, pages 695 - 701
COLD SPRING HARB PROTOC., vol. 2015, no. 11, November 2015 (2015-11-01), pages 951 - 969
SUZUKI K ET AL., NATURE, vol. 540, 2016, pages 144 - 149
CONKLIN BR ET AL., NATURE, vol. 363, 1993, pages 274 - 276
CONKLIN BR ET AL., MOL. PHARMACOLOGY, vol. 50, 1996, pages 885 - 890
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
Download PDF:
Previous Patent: ANGLE DETECTION SYSTEM AND ANGLE DETECTION METHOD
Next Patent: ANGLE DETECTION SYSTEM AND ANGLE DETECTION METHOD
Next Patent: ANGLE DETECTION SYSTEM AND ANGLE DETECTION METHOD