Title:
METHOD FOR ACOUSTIC PRESSURE COMBINED ASSISTED EXTRACTION OF PECTIN RICH IN RG-I
Document Type and Number:
WIPO Patent Application WO/2021/108977
Kind Code:
A1
Abstract:
The present invention relates to the technical field of functional sugars. Disclosed is a method for extracting pectin polysaccharides rich in RG-I, and specifically refers to a method for extracting pectin polysaccharides rich in RG-I based on an acoustic pressure combined technology. The main process is: dispersing sieved citrus peel powder in an alkaline solution containing sodium borohydride (20-50mM), and placing the system at a temperature of 25-40℃ to perform combined acoustic pressure treatment under the treatment conditions of a static pressure of 0.2-0.4 MPa, an ultrasonic intensity of 0.5-3.5 W/mL, and a treatment duration of 20-40 minutes; then, centrifuging the ultrasonically treated system to remove residue, and sequentially performing, on the obtained extract, ethanol precipitation (50%-80% v/v), precipitate dialysis (3000-3500 Da), and vacuum freeze-drying to obtain pectin polysaccharides. Compared with traditional pectin polysaccharide extraction methods, the pectin polysaccharides extracted by the method of the present invention are rich in RG-I type structural domains and have a better physiological activity, and the method requires mild product reaction conditions, is green and environmentally friendly, greatly shortens the preparation duration, and has high industrial application value.
Inventors:
CHEN SHIGUO (CN)
HU WEIWEI (CN)
YE XINGQIAN (CN)
CHENG HUAN (CN)
LIU DONGHONG (CN)
THUNTHACHA CHANTAPAKUL (TH)
HU WEIWEI (CN)
YE XINGQIAN (CN)
CHENG HUAN (CN)
LIU DONGHONG (CN)
THUNTHACHA CHANTAPAKUL (TH)
Application Number:
PCT/CN2019/122624
Publication Date:
June 10, 2021
Filing Date:
December 03, 2019
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C08B37/06
Domestic Patent References:
| WO2010037636A1 | 2010-04-08 | |||
| WO2013063251A1 | 2013-05-02 |
Foreign References:
| CN107141369A | 2017-09-08 | |||
| CN105542033A | 2016-05-04 | |||
| CN107629135A | 2018-01-26 | |||
| RU2339395C1 | 2008-11-27 | |||
| CN106893001A | 2017-06-27 |
Attorney, Agent or Firm:
HANGZHOU HUACHENLIANMING INTELLECTUAL PROPERTY AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
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