Title:
METHOD FOR AMPLIFYING NUCLEOTIDE SEQUENCE, AND SEQUENCE DETERMINATION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/033648
Kind Code:
A1
Abstract:
This method for amplifying a sequence adjacent to a specific sequence comprises: a step for annealing a first forward primer to a specific sequence to synthesize a complementary strand; a step for sequentially polymerically adding a first deoxynucleotide and a second deoxynucleotide to the 3' end of the complementary strand; a step for annealing a first reverse primer to a binding site of a polydeoxynucleotide strand composed of the first deoxynucleotide to synthesize a double-stranded DNA; a step for using the double-stranded DNA as a template to perform PCR in which a second forward primer and a first reverse primer which are complementary to the specific sequence are used; and a step for performing PCR, in which a third forward primer and a second reverse primer, which are complementary to the specific sequence, are used.
Inventors:
SAITO MASUMICHI (JP)
MOMOSE HARUKA (JP)
WADA YUSAKU (JP)
MATSUDAIRA TAKAHIRO (JP)
MOMOSE HARUKA (JP)
WADA YUSAKU (JP)
MATSUDAIRA TAKAHIRO (JP)
Application Number:
PCT/JP2020/030907
Publication Date:
February 25, 2021
Filing Date:
August 14, 2020
Export Citation:
Assignee:
DIRECTOR GENERAL OF NAT INSTITUTE OF INFECTIOUS DISEASES (JP)
FASMAC CO LTD (JP)
FASMAC CO LTD (JP)
International Classes:
C12Q1/686; C12Q1/6869; C12Q1/6876; C12N15/09
Domestic Patent References:
| WO2015111209A1 | 2015-07-30 | |||
| WO2012138549A1 | 2012-10-11 |
Foreign References:
| JP2004275037A | 2004-10-07 | |||
| JP2006166907A | 2006-06-29 | |||
| JP2002502605A | 2002-01-29 | |||
| JP2014511695A | 2014-05-19 | |||
| JP2020005549A | 2020-01-16 |
Other References:
LIU, TAIAN ET AL.: "A tailing genome walking method suitable for genomes with high local GC content", ANAL. BIOCHEM., vol. 441, 2013, pages 101 - 103, XP028711279, DOI: 10.1016/j.ab.2013.06.013
PARUZYNSKI, ANNA ET AL.: "Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing", NAT. PROTOC., vol. 5, 2010, pages 1379 - 1395, XP008135463, DOI: 10.1038/nprot.2010.87
RUDI, KUNT AND T FOSSHEIM, K S JAKOBSEN: "Restriction cutting independent method for cloning genomic DNA segments outside the boundaries of known sequences", BIOTECHNIQUES, vol. 27, no. 6, 1999, pages 1170 - 1172, 1176-1177, XP001247018
SAITO, MASUMICHI ET AL.: "A high-throughput detection method for the clonality of Human T- cell leukemia virus type-1-infected cells in vivo", INT. J. HEMATOL., vol. 112, 29 July 2020 (2020-07-29), pages 300 - 306, XP037230089, DOI: 10.1007/s12185-020-02935-5
YAMAUCHI, SHUNSUKE ET AL.: "O1-17-3: Development of a method in clonality analysis of HTLV-1 (RAIS1) for next-generation", JOURNAL OF THE JAPANESE SOCIETY FOR LABORATORY HEMATOLOGY, vol. 21, no. Suppl., 22 June 2020 (2020-06-22), pages S160, XP009534665, ISSN: 1347-2836
PARUZYNSKI A ET AL., NAT PROTOC., vol. 5, August 2010 (2010-08-01), pages 1379 - 1395
See also references of EP 4019648A4
PARUZYNSKI, ANNA ET AL.: "Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing", NAT. PROTOC., vol. 5, 2010, pages 1379 - 1395, XP008135463, DOI: 10.1038/nprot.2010.87
RUDI, KUNT AND T FOSSHEIM, K S JAKOBSEN: "Restriction cutting independent method for cloning genomic DNA segments outside the boundaries of known sequences", BIOTECHNIQUES, vol. 27, no. 6, 1999, pages 1170 - 1172, 1176-1177, XP001247018
SAITO, MASUMICHI ET AL.: "A high-throughput detection method for the clonality of Human T- cell leukemia virus type-1-infected cells in vivo", INT. J. HEMATOL., vol. 112, 29 July 2020 (2020-07-29), pages 300 - 306, XP037230089, DOI: 10.1007/s12185-020-02935-5
YAMAUCHI, SHUNSUKE ET AL.: "O1-17-3: Development of a method in clonality analysis of HTLV-1 (RAIS1) for next-generation", JOURNAL OF THE JAPANESE SOCIETY FOR LABORATORY HEMATOLOGY, vol. 21, no. Suppl., 22 June 2020 (2020-06-22), pages S160, XP009534665, ISSN: 1347-2836
PARUZYNSKI A ET AL., NAT PROTOC., vol. 5, August 2010 (2010-08-01), pages 1379 - 1395
See also references of EP 4019648A4
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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