Title:
METHOD FOR ANALYZING METABOLITES OF MICROORGANISMS
Document Type and Number:
WIPO Patent Application WO/2022/163417
Kind Code:
A1
Abstract:
Provided is a method by which the time required for analysis can be shortened and various substances produced by microorganisms can be analyzed. A method for analyzing metabolites of microorganisms according to the present invention comprises: a step for supplying a mobile phase containing carbon dioxide in a liquid state, a subcritical state, or a supercritical state to a container in which microorganisms cultured in a medium are housed together with the medium, and thereby moving, to the mobile phase, metabolite components of the microorganisms present in the microorganisms and the medium; a step for introducing, into a column, the mobile phase to which the metabolite components have been moved; and a step for mass-analyzing the metabolite components contained in the mobile phase which has passed through the column.
Inventors:
HORIE SHINNOSUKE (JP)
FUJITO YUKA (JP)
HASUNUMA TOMOHISA (JP)
YOSHIDA TAKANOBU (JP)
URAHATA ERIKA (JP)
FUJITO YUKA (JP)
HASUNUMA TOMOHISA (JP)
YOSHIDA TAKANOBU (JP)
URAHATA ERIKA (JP)
Application Number:
PCT/JP2022/001459
Publication Date:
August 04, 2022
Filing Date:
January 17, 2022
Export Citation:
Assignee:
SHIMADZU CORP (JP)
UNIV KOBE NAT UNIV CORP (JP)
AJINOMOTO KK (JP)
UNIV KOBE NAT UNIV CORP (JP)
AJINOMOTO KK (JP)
International Classes:
C12Q1/02; G01N30/04; G01N30/26
Domestic Patent References:
| WO2006118167A1 | 2006-11-09 | |||
| WO2019163245A1 | 2019-08-29 | |||
| WO2018155272A1 | 2018-08-30 | |||
| WO2016084963A1 | 2016-06-02 |
Foreign References:
| JP2019184502A | 2019-10-24 | |||
| JP2013250186A | 2013-12-12 | |||
| JP2019184502A | 2019-10-24 |
Other References:
ANONYMOUS: "Developing technology to analyze cell metabolites with high speed and high accuracy", NEDO, 17 February 2021 (2021-02-17), XP055953730, Retrieved from the Internet
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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