Title:
METHOD FOR ASSESSING INFLAMMATORY RETINAL DISEASE, INFLAMMATORY RETINAL DISEASE THERAPEUTIC, AND SCREENING METHOD FOR INFLAMMATORY RETINAL DISEASE THERAPEUTICS
Document Type and Number:
WIPO Patent Application WO/2022/113887
Kind Code:
A1
Abstract:
A purpose of the present invention is to provide a novel assessment method that enables early detection, assessment of severity, and assessment of therapeutic effect, etc., of chronic inflammatory retinal diseases including age-related macular degeneration. Another purpose is to provide a novel therapeutic for inflammatory retinal diseases including age-related macular degeneration and to provide a novel screening method for inflammatory retinal disease therapeutics. The present invention is a method for assessing inflammatory retinal diseases associated with tissue inflammation, with extracellular vesicles (EV) produced by the retinal pigment epithelial cells (RPE) of a subject being employed as an indicator. This assessment method is characterized in that the profile of miRNA contained in the extracellular vesicles (EV) (especially miR494-3p and/or miR1246) is employed as an indicator.
Inventors:
HAMURO JUNJI (JP)
SOTOZONO CHIE (JP)
KINOSHITA SHIGERU (JP)
SOTOZONO CHIE (JP)
KINOSHITA SHIGERU (JP)
Application Number:
PCT/JP2021/042479
Publication Date:
June 02, 2022
Filing Date:
November 18, 2021
Export Citation:
Assignee:
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
International Classes:
C12Q1/68; A61K31/7088; A61K39/395; A61K45/00; A61K48/00; A61P27/02; A61P29/00; A61P43/00; C12N15/113; C12Q1/02; G01N33/15
Domestic Patent References:
| WO2014028861A1 | 2014-02-20 |
Foreign References:
| US20140322720A1 | 2014-10-30 | |||
| JP2016513095A | 2016-05-12 | |||
| JP2019521963A | 2019-08-08 |
Other References:
AHN J.Y., DATTA S., BANDEIRA E., CANO M., MALLICK E., RAI U., POWELL B., TIAN J., WITWER K.W., HANDA J.T., PAULAITIS M.E.: "Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria", BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 1865, no. 4, 1 April 2021 (2021-04-01), AMSTERDAM, NL , pages 129598, XP055932654, ISSN: 0304-4165, DOI: 10.1016/j.bbagen.2020.129598
GUM-YONG KANG, JOO YOUNG BANG, AE JIN CHOI, JEEHYUN YOON, WON-CHUL LEE, SOYOUNG CHOI, SOOJIN YOON, HYUNG CHAN KIM, JE-HYUN BAEK, H: "Exosomal Proteins in the Aqueous Humor as Novel Biomarkers in Patients with Neovascular Age-related Macular Degeneration", JOURNAL OF PROTEOME RESEARCH, AMERICAN CHEMICAL SOCIETY, vol. 13, no. 2, 7 February 2014 (2014-02-07), pages 581 - 595, XP055581283, ISSN: 1535-3893, DOI: 10.1021/pr400751k
YAMAWAKI TAKAHIRO, ITO EIKO, MUKAI ATSUSHI, UENO MORIO, YAMADA JUN, SOTOZONO CHIE, KINOSHITA SHIGERU, HAMURO JUNJI: "The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, ASSOCIATION FOR RESEARCH IN VISION AND OPHTHALMOLOGY, US, vol. 57, no. 14, 7 November 2016 (2016-11-07), US , pages 5945, XP055932658, ISSN: 1552-5783, DOI: 10.1167/iovs.16-20604
MUKAI ATSUSHI; OTSUKI YOHEI; ITO EIKO; FUJITA TOMOKO; UENO MORIO; MAEDA TADAO; KINOSHITA SHIGERU; SOTOZONO CHIE; HAMURO JUNJI: "Mitochondrial miRNA494-3p in extracellular vesicles participates in cellular interplay of iPS-Derived human retinal pigment epithelium with macrophages", EXPERIMENTAL EYE RESEARCH, ACADEMIC PRESS LTD., LONDON., vol. 208, 14 May 2021 (2021-05-14), LONDON. , XP086628025, ISSN: 0014-4835, DOI: 10.1016/j.exer.2021.108621
GUM-YONG KANG, JOO YOUNG BANG, AE JIN CHOI, JEEHYUN YOON, WON-CHUL LEE, SOYOUNG CHOI, SOOJIN YOON, HYUNG CHAN KIM, JE-HYUN BAEK, H: "Exosomal Proteins in the Aqueous Humor as Novel Biomarkers in Patients with Neovascular Age-related Macular Degeneration", JOURNAL OF PROTEOME RESEARCH, AMERICAN CHEMICAL SOCIETY, vol. 13, no. 2, 7 February 2014 (2014-02-07), pages 581 - 595, XP055581283, ISSN: 1535-3893, DOI: 10.1021/pr400751k
YAMAWAKI TAKAHIRO, ITO EIKO, MUKAI ATSUSHI, UENO MORIO, YAMADA JUN, SOTOZONO CHIE, KINOSHITA SHIGERU, HAMURO JUNJI: "The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, ASSOCIATION FOR RESEARCH IN VISION AND OPHTHALMOLOGY, US, vol. 57, no. 14, 7 November 2016 (2016-11-07), US , pages 5945, XP055932658, ISSN: 1552-5783, DOI: 10.1167/iovs.16-20604
MUKAI ATSUSHI; OTSUKI YOHEI; ITO EIKO; FUJITA TOMOKO; UENO MORIO; MAEDA TADAO; KINOSHITA SHIGERU; SOTOZONO CHIE; HAMURO JUNJI: "Mitochondrial miRNA494-3p in extracellular vesicles participates in cellular interplay of iPS-Derived human retinal pigment epithelium with macrophages", EXPERIMENTAL EYE RESEARCH, ACADEMIC PRESS LTD., LONDON., vol. 208, 14 May 2021 (2021-05-14), LONDON. , XP086628025, ISSN: 0014-4835, DOI: 10.1016/j.exer.2021.108621
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES (JP)
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