Title:
METHOD FOR CLEAVING DISULFIDE BOND IN PROTEIN AND DEVICE FOR CLEAVING DISULFIDE BOND IN PROTEIN
Document Type and Number:
WIPO Patent Application WO/2021/261509
Kind Code:
A1
Abstract:
A method for cleaving a disulfide bond in a protein, the method comprising: a cleavage step for cleaving a disulfide bond in a protein present in a reaction system by reducing the disulfide bond using a reduced redox protein (step S104); and a reduction step for providing an electron from an electrode connected to an external power supply outside the reaction system to an oxidized redox protein obtained by oxidation of the reduced redox protein in the cleavage step, and thereby reducing the oxidized redox protein to obtain the reduced redox protein (step S103).
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Inventors:
HATSUGAI NORIYUKI
OKUMURA YASUAKI
WAYAMA FUMIYA
OKUMURA YASUAKI
WAYAMA FUMIYA
Application Number:
PCT/JP2021/023750
Publication Date:
December 30, 2021
Filing Date:
June 23, 2021
Export Citation:
Assignee:
PANASONIC IP MAN CO LTD (JP)
International Classes:
A23J3/04; C07K1/00; A23J3/14; A23L33/18
Foreign References:
| JP2009002689A | 2009-01-08 | |||
| CN109652402A | 2019-04-19 | |||
| JP2001520027A | 2001-10-30 | |||
| JPH03228669A | 1991-10-09 | |||
| JP2001520027A |
Other References:
KEISUKE YOSHIDA ET AL.: "Biochemical Journal", vol. 474, 2017, PORTLAND PRESS, article "Distinct electron transfer from ferredoxin-thioredoxin reductase to multiple thioredoxin isoforms in chloroplasts", pages: 1347 - 1360
See also references of EP 4169932A4
See also references of EP 4169932A4
Attorney, Agent or Firm:
NII, Hiromori et al. (JP)
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