Title:
METHOD FOR CONSTRUCTING OENOCOCCUS OENI ENGINEERING BACTERIA AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/103812
Kind Code:
A1
Abstract:
Provided are a method for constructing Oenococcus oeni engineering bacteria and the use thereof. The construction method comprises the following steps: (1) preparing a lipase gene lipase; (2) preparing a homologous arm gene having the lipase gene; (3) preparing a plasmid pMG36e-lipase; (4) preparing a nisl fragment; (5) preparing a linear fragment containing no erythromycin sequence; (6) preparing an expression vector pMG36n-lipase; and (7) transforming the expression vector pMG36n-lipase into Oenococcus oeni competent cells to obtain the Oenococcus oeni engineering bacteria. The Oenococcus oeni engineering bacteria with the function of degrading esters obtained by means of the construction method can be applied to the production of wine, such that adverse effects on the taste and aroma of the wine caused by excessive esters in the wine-making process are avoided in order to reduce the preservation time of the wine and shorten the production cycle of the wine while achieving the same taste.
Inventors:
HE XI (CN)
HAN NING (CN)
HOU DONGDONG (CN)
ZHAO XINJIE (CN)
HAN NING (CN)
HOU DONGDONG (CN)
ZHAO XINJIE (CN)
Application Number:
PCT/CN2020/118773
Publication Date:
June 03, 2021
Filing Date:
September 29, 2020
Export Citation:
Assignee:
UNIV QILU TECHNOLOGY (CN)
International Classes:
C12N15/55; C12G1/022; C12N1/21; C12N15/74; C12R1/01
Foreign References:
| CN110846331A | 2020-02-28 | |||
| CN104845811A | 2015-08-19 | |||
| CN102586314A | 2012-07-18 | |||
| CN108486140A | 2018-09-04 |
Other References:
YIN JIANHONG: "Expression of Candida Antarctica Lipase B in Lactococcus LactisMG1363", CHINESE MASTER’S THESES FULL-TEXT DATABASE, ENGINEERING SCIENCE AND TECHNOLOGY I, 1 June 2012 (2012-06-01), XP055815694
ZHANG YAN, GONG AI-HUA, JIN JIE, SHAO GEN-BAO, PENG WAN-XIN: "Application of homologous recombination in vitro to construct pcDNA3． 1-NGF and pcDNA3． 1-TrkA", JOURNAL OF JIANGSU UNIVERSITY(MEDICINE EDITION), vol. 21, no. 5, 1 September 2011 (2011-09-01), pages 390 - 401, XP055815691, ISSN: 1671-7783, DOI: 10.13312/j.issn.1671-7783.2011.05.002
ZHANG YAN, GONG AI-HUA, JIN JIE, SHAO GEN-BAO, PENG WAN-XIN: "Application of homologous recombination in vitro to construct pcDNA3． 1-NGF and pcDNA3． 1-TrkA", JOURNAL OF JIANGSU UNIVERSITY(MEDICINE EDITION), vol. 21, no. 5, 1 September 2011 (2011-09-01), pages 390 - 401, XP055815691, ISSN: 1671-7783, DOI: 10.13312/j.issn.1671-7783.2011.05.002
Attorney, Agent or Firm:
ZHUSEN INTELLECTUAL PROPERTY AGENCY OFFICE (CN)
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