Title:
METHOD FOR DETECTING ENZYMATIC REACTION PRODUCT
Document Type and Number:
WIPO Patent Application WO/2019/168200
Kind Code:
A1
Abstract:
As a technique applicable to highly sensitive detection of a pathogenic microorganism such as influenza virus, provided is a method which comprises reacting an enzyme with a substance, said substance serving as a substrate in a reaction by the enzyme, in a hydrophilic solvent being in interfacial contact with a hydrophobic solvent and then detecting the reaction product formed and released directly from the substrate, wherein the hydrophilic solvent contains a buffering substance and/or trimethylamine oxide (TMAO).
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Inventors:
TABATA KAZUHITO (JP)
MINAGAWA YOSHIHIRO (JP)
NOJI HIROYUKI (JP)
MINAGAWA YOSHIHIRO (JP)
NOJI HIROYUKI (JP)
Application Number:
PCT/JP2019/008411
Publication Date:
September 06, 2019
Filing Date:
March 04, 2019
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12Q1/00; C12Q1/02; C12Q1/34; C12Q1/70
Domestic Patent References:
| WO2012121310A1 | 2012-09-13 | |||
| WO2018181488A1 | 2018-10-04 | |||
| WO2018043733A1 | 2018-03-08 | |||
| WO2016006208A1 | 2016-01-14 |
Foreign References:
| JPH1075779A | 1998-03-24 | |||
| JP2006271396A | 2006-10-12 | |||
| JP2010524505A | 2010-07-22 | |||
| JP2002508193A | 2002-03-19 | |||
| JPS5676049A | 1981-06-23 | |||
| JP2002506626A | 2002-03-05 | |||
| JP2000509971A | 2000-08-08 | |||
| JP2013101110A | 2013-05-23 | |||
| JP2014514930A | 2014-06-26 |
Attorney, Agent or Firm:
UMEDA Shinsuke (JP)
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