Title:
METHOD FOR DETECTING PROTEIN-PROTEIN INTERACTIONS AND A KIT THEREFOR
Document Type and Number:
WIPO Patent Application WO2000005410
Kind Code:
A3
Abstract:
A method for detecting an interaction between two tester proteins. In a cell incapable of activating a Ras protein, two nucleic acid sequences are expressed. One sequence encodes for a fusion protein comprising a mutant Ras protein incapable of localizing at the cell membrane and not requiring an exchange factor fused to one of the tester proteins. The other sequence encodes for the other tester protein fused to a plasma membrane localization domain. An interaction between the two fusion proteins leads to the expression of a functional Ras protein that is detected as an altered cell phenotype.
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Inventors:
ARONHEIM AMI (IL)
Application Number:
PCT/IL1999/000404
Publication Date:
June 29, 2000
Filing Date:
July 22, 1999
Export Citation:
Assignee:
TECHNION RES & DEV FOUNDATION (IL)
ARONHEIM AMI (IL)
ARONHEIM AMI (IL)
International Classes:
C12Q1/68; (IPC1-7): C12N15/10; C12Q1/68
Foreign References:
| US5776689A | 1998-07-07 |
Other References:
ARONHEIM A. ET AL.,: "isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions", MOL. CELL. BIOLOGY, vol. 17, no. 6, - June 1997 (1997-06-01), pages 3094 - 3102, XP002135900
BHATTACHARYA S. ET AL.,: "Ras membrane targeting is essential for glucose signaling but not for viability in yeast", PROC. NATL. ACAD. SCI. USA, vol. 92, - March 1995 (1995-03-01), pages 2984 - 2988, XP002135901
BRODER Y.C. ET AL.,: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, vol. 8, no. 20, - 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902
BHATTACHARYA S. ET AL.,: "Ras membrane targeting is essential for glucose signaling but not for viability in yeast", PROC. NATL. ACAD. SCI. USA, vol. 92, - March 1995 (1995-03-01), pages 2984 - 2988, XP002135901
BRODER Y.C. ET AL.,: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, vol. 8, no. 20, - 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902
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