METHOD FOR DETECTING SINGLE-STRANDED RNA VIRUS

Title:

METHOD FOR DETECTING SINGLE-STRANDED RNA VIRUS

Document Type and Number:

WIPO Patent Application WO/2021/172370

Kind Code:

A1

Abstract:

According to one embodiment of the present invention, a method for detecting a single-stranded RNA virus in a sample comprises a step for contacting a primer set with the sample and performing a reverse transcription loop-mediated isothermal amplification. This primer set is designed on the basis of the base sequence of a target RNA and the base sequence of a nucleic acid that is complementary to the target RNA and contains the following (i) to (v): (i) an FIP primer; (ii) a BIP primer; (iii) an F3 primer that is an outer primer; (iv) a B3 primer that is an outer primer; and (v) one or more additional outer primers, each additional outer primer having the same base sequence as the base sequence of an arbitrary region located on the 5' terminal side of region B3 or region F3 in the nucleic acid that is complementary to the target RNA. According to the present invention, a single-stranded RNA virus can be detected at a high sensitivity.

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Inventors:

YAMAMOTO MACHIKO (JP)
YOKONO KOTA (JP)
SEMBA SYOUHEI (JP)
MICHIYUKI SATORU (JP)

Application Number:

PCT/JP2021/006907

Publication Date:

September 02, 2021

Filing Date:

February 24, 2021

Export Citation:

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Assignee:

EIKEN CHEMICAL (JP)

International Classes:

C12Q1/6844; C12Q1/6876

Domestic Patent References:

WO2000028082A1	2000-05-18
WO2002024902A1	2002-03-28
WO2018042598A1	2018-03-08

Foreign References:

CN106222298A	2016-12-14
CN103074445A	2013-05-01

Other References:

CHEN LEI, FAN XUE-ZHENG, WANG QIN, XU LU, ZHAO QI-ZU, ZHOU YUAN-CHEN, LIU JUN, TANG BO, ZOU XING-QI: "A Novel RT-LAMP Assay for Rapid and Simple Detection of Classical Swine Fever Virus", VIROLOGICA SINICA, vol. 25, no. 1, 1 February 2010 (2010-02-01), DE, pages 59 - 64, XP055850539, ISSN: 1674-0769, DOI: 10.1007/s12250-010-3043-2
BAZARTSEREN B.S. INOUEA. YAMADA ET AL.: "Rapid Detection of Rabies Virus by Reverse Transcription Loop-Mediated Isothermal Amplification", JPN. J. INFECT. DIS., vol. 62, 2009, pages 187 - 191
See also references of EP 4112743A4

Attorney, Agent or Firm:

HASEGAWA Yoshiki et al. (JP)

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