Title:
METHOD FOR DETERMINING QUANTITY OF BIOLOGICAL MATERIAL IN TISSUE SECTION
Document Type and Number:
WIPO Patent Application WO/2015/045962
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for detecting a specific tissue/cell in a sampled tissue section and accurately specifying an expression position and expression amount of a biological material of interest that is expressed on the specific tissue/cell. This method for determining the quantity of a biological material in a tissue section includes (1) performing immunostaining (first immunostaining) capable of bright field observation, for specifically staining a first biological material in the tissue section, (2) performing immunostaining (second immunostaining) through use of nanoparticles encapsulating a phosphor for specifically staining a second biological material in the tissue section, (3) specifying the expression position of the second biological material in the tissue section by comparing the position of a staining image of the first immunostaining and the position of a staining image of the second immunostaining, and (4) specifying the expression amount of the second biological material by measuring the amount of fluorescence of the staining image of the second immunostaining.
Inventors:
OKADA FUMINORI (JP)
AIMIYA TAKUJI (JP)
GONDA KOHSUKE (JP)
OHUCHI NORIAKI (JP)
WATANABE MIKA (JP)
AIMIYA TAKUJI (JP)
GONDA KOHSUKE (JP)
OHUCHI NORIAKI (JP)
WATANABE MIKA (JP)
Application Number:
PCT/JP2014/074420
Publication Date:
April 02, 2015
Filing Date:
September 16, 2014
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
UNIV TOHOKU (JP)
UNIV TOHOKU (JP)
International Classes:
G01N33/53; G01N21/64; G01N33/48; G01N33/536
Domestic Patent References:
| WO2012029752A1 | 2012-03-08 |
Foreign References:
| JP2013088296A | 2013-05-13 | |||
| JP2008268167A | 2008-11-06 |
Other References:
VILPPU TUOMINEN ET AL.: "ImmunoRatio-F: image analysis of Ki-67 using cytokeratin immunofluorescence correction", 11TH EUROPEAN CONGRESS ON TELEPATHOLOGY AND 5TH INTERNATIONAL CONGRESS ON VIRTUAL MICROSCOPY, XP009182621, Retrieved from the Internet
VILPPU J TUOMINEN ET AL.: "ImmunoRatio: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor(PR), and Ki-67", BREAST CANCER RESEARCH, vol. 12, 2010, pages R56, XP021085368
TUOMINEN, V.; RUOTOISTEUMAKI, S.; VIITANEN, A.; JUMPPANEN, M.; ISOLA, J: "ImmunoRatio-F: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67", BREAST CANCER RESEARCH, vol. 12, 2010, pages R56, XP021085368, DOI: doi:10.1186/bcr2615
VILPPU J TUOMINEN ET AL.: "ImmunoRatio: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor(PR), and Ki-67", BREAST CANCER RESEARCH, vol. 12, 2010, pages R56, XP021085368
TUOMINEN, V.; RUOTOISTEUMAKI, S.; VIITANEN, A.; JUMPPANEN, M.; ISOLA, J: "ImmunoRatio-F: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67", BREAST CANCER RESEARCH, vol. 12, 2010, pages R56, XP021085368, DOI: doi:10.1186/bcr2615
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
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